Novus Biologicals products are now on bio-techne.com

Actin Alpha 1 Cardiac Muscle Antibody (959615) [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 2 ...read more
alpha -Cardiac Actin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat alpha -Cardiac Actin Monoclonal Antibody (Catalog # MAB93081) at 1.7 µg/mL for ...read more
Simple Western lane view shows lysates of Human Heart, loaded at 0.2 mg/mL. A specific band was detected for alpha -Cardiac Actin at approximately 49 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clone
959615
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Actin Alpha 1 Cardiac Muscle Antibody (959615) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human alpha -Cardiac Actin
Asp3-Phe377
Accession # P68032
Specificity
Detects human alpha -Cardiac Actin in direct ELISAs. Detects human,mouse, and rat alpha -Cardiac Actin in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1.7-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using MAB93081.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Actin Alpha 1 Cardiac Muscle Antibody (959615) [Unconjugated]

  • ACTC
  • ACTC1
  • actin, alpha, cardiac muscle 1
  • alpha-Cardiac Actin
  • ASD5
  • Cardiac alpha-Actin
  • CMD1R
  • CMH11
  • LVNC4

Background

Actin, alpha cardiac muscle 1 (ACTC1) is a 377 aa cytoskeletal filament in cardiac muscle which interacts with the 50-KDa domain of the myosin motor domain in cardiac muscle contraction. Actin is highly conserved across species, and differs from skeletal muscle actin (ACTA1) by only four amino acids. Mutations in the ACTC1 gene are linked to familial hypertrophy cardiomyopathy, atrial septal defects, arrhythmia, and chronic inflammatory cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB9096
Species: Hu
Applications: IHC, WB
NBP2-35336
Species: Hu, Mu
Applications: IHC, IHC-P, WB
MAB1874
Species: Hu
Applications: ICC, IHC
AF7439
Species: Hu
Applications: IHC, WB
NBP1-33716
Species: Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-88068
Species: Hu
Applications: IHC, IHC-P
NBP1-85541
Species: Hu, Mu
Applications: IHC, IHC-Fr, IHC-P, WB
NBP1-88071
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P
NBP2-37958
Species: Hu
Applications: IHC, IHC-P
NBP1-06983
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, PEP-ELISA, WB
NBP2-25151
Species: Hu, Pm, Mu, Rt
Applications: ICC/IF, WB
MAB4470
Species: All-Multi
Applications: ICC, IHC, WB
AF3844
Species: Hu, Mu
Applications: IHC
H00011155-M06
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IP, KD, WB
NB300-284
Species: Ca, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-19807
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00051086-D01P
Species: Hu, Mu
Applications: ICC/IF, WB
MAB93081
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC

Publications for Actin Alpha 1 Cardiac Muscle Antibody (MAB93081)(2)

Reviews for Actin Alpha 1 Cardiac Muscle Antibody (MAB93081) (0)

There are no reviews for Actin Alpha 1 Cardiac Muscle Antibody (MAB93081). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Actin Alpha 1 Cardiac Muscle Antibody (MAB93081) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Actin Alpha 1 Cardiac Muscle Antibody (959615) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Uniprot