Novus Biologicals products are now on bio-techne.com

Actin Alpha 1 Cardiac Muscle Antibody (22D3) [Janelia Fluor® 585]

Name: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [Janelia Fluor&#174; 585]
Brand: Novus Biologicals
SKU: NBP1-97725JF585
Price: 549 USD

Submit a Review

Images

There are currently no images for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725JF585).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary

Reactivity	Hu, Mu, Rt, Po, Bv, Gt, Rb, ZeSpecies Glossary
Applications	WB, ELISA, ICC/IF, IHC
Clone	22D3
Clonality	Monoclonal
Host	Mouse
Conjugate	Janelia Fluor 585

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP1-97725AF350

Alexa Fluor 405 NBP1-97725AF405

Alexa Fluor 488 NBP1-97725AF488

Alexa Fluor 532 NBP1-97725AF532

Alexa Fluor 594 NBP1-97725AF594

Alexa Fluor 647 NBP1-97725AF647

Alexa Fluor 700 NBP1-97725AF700

Alexa Fluor 750 NBP1-97725AF750

Biotin NBP1-97725B

CoraFluor 1 NBP1-97725CL1

DyLight 350 NBP1-97725UV

DyLight 405 NBP1-97725V

DyLight 488 NBP1-97725G

DyLight 550 NBP1-97725R

DyLight 594 NBP1-97725DL594

DyLight 650 NBP1-97725C

DyLight 680 NBP1-97725FR

DyLight 755 NBP1-97725IR

FITC NBP1-97725F

HRP NBP1-97725H

Janelia Fluor 525 NBP1-97725JF525

Janelia Fluor 549 NBP1-97725JF549

Janelia Fluor 635 NBP1-97725JF635

Janelia Fluor 646 NBP1-97725JF646

Janelia Fluor 669 NBP1-97725JF669

Unconjugated NBP1-97725

View Available Formulations

Catalog# & Formulation	Size	Price
Standard Formulation NBP1-97725

BSA Free NBP1-97725

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-97725AF350
Alexa Fluor 405	NBP1-97725AF405
Alexa Fluor 488	NBP1-97725AF488
Alexa Fluor 532	NBP1-97725AF532
Alexa Fluor 594	NBP1-97725AF594
Alexa Fluor 647	NBP1-97725AF647
Alexa Fluor 700	NBP1-97725AF700
Alexa Fluor 750	NBP1-97725AF750
Biotin	NBP1-97725B
CoraFluor 1	NBP1-97725CL1
DyLight 350	NBP1-97725UV
DyLight 405	NBP1-97725V
DyLight 488	NBP1-97725G
DyLight 550	NBP1-97725R
DyLight 594	NBP1-97725DL594
DyLight 650	NBP1-97725C
DyLight 680	NBP1-97725FR
DyLight 755	NBP1-97725IR
FITC	NBP1-97725F
HRP	NBP1-97725H
Janelia Fluor 525	NBP1-97725JF525
Janelia Fluor 549	NBP1-97725JF549
Janelia Fluor 635	NBP1-97725JF635
Janelia Fluor 646	NBP1-97725JF646
Janelia Fluor 669	NBP1-97725JF669
Unconjugated	NBP1-97725

Actin Alpha 1 Cardiac Muscle Antibody (22D3) [Janelia Fluor® 585] Summary

Immunogen	Derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of Actin Alpha 1 Cardiac Muscle with an acetylated N-terminus (Ac-DDEETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
Specificity	This antibody is highly specific for actin alpha 1 (cardiac), and does not cross react with other actin isoforms.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	ACTC1
Purity	Protein A or G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions	ELISA Immunocytochemistry/ Immunofluorescence Immunohistochemistry Immunohistochemistry-Frozen Immunohistochemistry-Paraffin Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined..

Reactivity Notes

The epitope recognized by this antibody is highly conserved; the antibody therefore cross-reacts with many species

Bovine reactivity reported in scientific literature (PMID: 30591979). Mouse reactivity reported in scientific literature (PMID: 28624189).

Packaging, Storage & Formulations

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide
Purity	Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Actin Alpha 1 Cardiac Muscle Antibody (22D3) [Janelia Fluor® 585]

ACTC
ACTC1
actin, alpha, cardiac muscle 1
alpha-Cardiac Actin
ASD5
Cardiac alpha-Actin
CMD1R
CMH11
LVNC4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MYH7 Antibody
(940906) [Unconjugate

MAB9096

Western Blot MYH7 Antibody (940906) [Unconjugated]

Species: Hu

Applications: IHC, WB

Troponin I Type 3
(cardiac) Antibody (2 ...

NBP2-35336

Immunohistochemistry-Paraffin Troponin I Type 3 (cardiac) Antibody (2C9) - BSA Free

Species: Hu, Mu

Applications: IHC, IHC-P, WB

Cardiac Troponin T
Antibody (200805) [Un ...

MAB1874

Immunohistochemistry Cardiac Troponin T Antibody (200805) [Unconjugated] - (Cardiac)

(17 Publications)

Species: Hu

Applications: ICC, IHC

MyBPC3 Antibody

AF7439

Species: Hu

Applications: IHC, WB

Tropomyosin-1
Antibody

NBP1-33716

Immunohistochemistry-Paraffin Tropomyosin-1 Antibody

Species: Hu, Mu, Po, Rt

Applications: ICC/IF, IHC, IHC-P, WB

Myosin light chain
3 Antibody

NBP1-88068

Species: Hu

Applications: IHC, IHC-P

Myosin Light Chain
2 Antibody

NBP1-85541

(1 Review)

(2 Publications)

Species: Hu, Mu

Applications: IHC, IHC-Fr, IHC-P, WB

Titin Antibody

NBP1-88071

(4 Publications)

Species: Hu, Rt

Applications: ICC/IF, IHC, IHC-P

Troponin C
(cardiac) Antibody

NBP2-37958

Species: Hu

Applications: IHC, IHC-P

CSRP3 Antibody

NBP1-06983

Immunohistochemistry-Paraffin CSRP3 Antibody

(2 Publications)

Species: Hu, Mu, Rt

Applications: ICC/IF, IHC, IHC-P, IP, PEP-ELISA, WB

Lamin A + C
Antibody (4C4)

NBP2-25151

(2 Publications)

Species: Hu, Pm, Mu, Rt

Applications: ICC/IF, WB

Myosin Heavy Chain
Antibody (MF20) [Unco ...

MAB4470

Immunocytochemistry Myosin Heavy Chain Antibody (MF20) [Unconjugated]

(1 Review)

(165 Publications)

Species: All-Multi

Applications: ICC, IHC, WB

Desmin Antibody
[Unconjugated]

AF3844

Immunocytochemistry/ Immunofluorescence Desmin Antibody [Unconjugated]

(2 Reviews)

(34 Publications)

Species: Hu, Mu

Applications: IHC

LDB3 Antibody (3C8)

H00011155-M06

Immunocytochemistry/ Immunofluorescence LDB3 Antibody (3C8)

(5 Publications)

Species: Hu, Mu, Rt

Applications: ELISA, ICC/IF, IP, KD, WB

MYH6 Antibody
(3-48) - Azide and BS ...

NB300-284

Immunohistochemistry-Paraffin MYH6 Antibody (3-48) - Azide and BSA Free

(2 Reviews)

(12 Publications)

Species: Ca, Hu, Mu, Rt

Applications: ICC/IF, IHC, IHC-P, WB

Phospholamban
Antibody

NBP2-19807

Immunohistochemistry-Paraffin Phospholamban Antibody

(3 Publications)

Species: Hu, Mu, Rt

Applications: ICC/IF, IHC, IHC-P, WB

TNNI3K Antibody

H00051086-D01P

Species: Hu, Mu

Applications: ICC/IF, WB

Actin Alpha 1
Cardiac Muscle Antibo ...

NBP1-97725JF585

Species: Hu, Mu, Rt, Po, Bv, Gt, Rb, Ze

Applications: WB, ELISA, ICC/IF, IHC

Publications for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725JF585) (0)

There are no publications for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725JF585).

By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725JF585) (0)

There are no reviews for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725JF585). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.

Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

ICC/IF Video Protocol

FAQs for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725JF585) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

Isotype Controls

Additional Actin Alpha 1 Cardiac Muscle Products

Research Areas for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725JF585)

Find related products by research area.

Blogs on Actin Alpha 1 Cardiac Muscle

There are no specific blogs for Actin Alpha 1 Cardiac Muscle, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Myosin Heavy Chain was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Myosin Heavy Chain Antibody (MF20) [Unconjugated]
MAB4470

Desmin was detected in immersion fixed frozen sections of mouse skeletal muscle using Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to z-lines. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Desmin Antibody [Unconjugated]
AF3844

Contact Information

Product PDFs

SDS

PDF Datasheet

Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

Volume

Mass

Concentration

Review this Product

Be the first to review our Actin Alpha 1 Cardiac Muscle Antibody (22D3) [Janelia Fluor® 585] and receive a gift card or discount.

Bioinformatics

Gene Symbol

ACTC1

Popular Products	Company Information	Support	Stay Connected
Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

Visit our other sites

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Actin Alpha 1 Cardiac Muscle Antibody (22D3) [Janelia Fluor® 585]

Species Glossary

Images

Product Details

Order Details

Actin Alpha 1 Cardiac Muscle Antibody (22D3) [Janelia Fluor® 585] Summary

Applications/Dilutions

Reactivity Notes

Packaging, Storage & Formulations

Notes

Alternate Names for Actin Alpha 1 Cardiac Muscle Antibody (22D3) [Janelia Fluor® 585]

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725JF585) (0)

Reviews for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725JF585) (0)

Product General Protocols

Video Protocols

FAQs for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725JF585) (0)

Secondary Antibodies

Isotype Controls

Additional Actin Alpha 1 Cardiac Muscle Products

Research Areas for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725JF585)

Blogs on Actin Alpha 1 Cardiac Muscle

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics