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Actin Alpha 1 Cardiac Muscle Antibody (22D3) [DyLight 550]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Gt, Rb, ZeSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
22D3
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 550

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Actin Alpha 1 Cardiac Muscle Antibody (22D3) [DyLight 550] Summary

Immunogen
Derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of Actin Alpha 1 Cardiac Muscle with an acetylated N-terminus (Ac-DDEETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
Specificity
This antibody is highly specific for actin alpha 1 (cardiac), and does not cross react with other actin isoforms.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACTC1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined..

Reactivity Notes

The epitope recognized by this antibody is highly conserved; the antibody therefore cross-reacts with many species

Bovine reactivity reported in scientific literature (PMID: 30591979). Mouse reactivity reported in scientific literature (PMID: 28624189).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Actin Alpha 1 Cardiac Muscle Antibody (22D3) [DyLight 550]

  • ACTC
  • ACTC1
  • actin, alpha, cardiac muscle 1
  • alpha-Cardiac Actin
  • ASD5
  • Cardiac alpha-Actin
  • CMD1R
  • CMH11
  • LVNC4

Background

Actin, alpha cardiac muscle 1 (ACTC1) is a 377 aa cytoskeletal filament in cardiac muscle which interacts with the 50-KDa domain of the myosin motor domain in cardiac muscle contraction. Actin is highly conserved across species, and differs from skeletal muscle actin (ACTA1) by only four amino acids. Mutations in the ACTC1 gene are linked to familial hypertrophy cardiomyopathy, atrial septal defects, arrhythmia, and chronic inflammatory cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725R) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725R) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACTC1