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ACSL1 Antibody

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Independent Antibodies: Western Blot: ACSL1 Antibody [NBP1-89271] - Analysis using Anti-ACSL1 antibody NBP1-89271 (A) shows similar pattern to independent antibody NBP1-89270 (B).
Immunocytochemistry/ Immunofluorescence: ACSL1 Antibody [NBP1-89271] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Western Blot: ACSL1 Antibody [NBP1-89271] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human kidney, liver, soft tissues and tonsil using Anti-ACSL1 antibody NBP1-89271 (A) shows similar protein ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Analysis in human liver and tonsil tissues. Corresponding ACSL1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human tonsil shows no cytoplasmic positivity in germinal center cells as expected.
Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human soft tissues shows strong cytoplasmic positivity in adipocytes.
Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ACSL1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YATRPKPLKPPCDLSMQSVEVAGSGGARRSALLDSDEPLVYFYDDVTTLYEGFQRGIQVSNNGPCLGSRKPDQPYEWLSYKQVAELSE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACSL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ACSL1 Protein (NBP1-89271PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ACSL1 Antibody

  • ACS1LACS 2
  • Acyl-CoA synthetase 1
  • acyl-CoA synthetase long-chain family member 1
  • EC 6.2.1
  • FACL1EC 6.2.1.3
  • fatty-acid-Coenzyme A ligase, long-chain 1
  • LACS 1
  • LACSlong-chain 2
  • lignoceroyl-CoA synthase
  • Long-chain acyl-CoA synthetase 1
  • Long-chain acyl-CoA synthetase 2
  • Long-chain fatty acid-CoA ligase 2
  • long-chain fatty-acid-coenzyme A ligase 1
  • long-chain-fatty-acid--CoA ligase 1
  • Palmitoyl-CoA ligase 1
  • Palmitoyl-CoA ligase 2
  • paltimoyl-CoA ligase 1

Background

Long-chain-fatty-acid--CoA ligase 1, or ACSL1, is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. These proteins play an important role in lipid biosynthesis and fatty acid degradation. Like other isozymes of this family, ALCS1 converts free long-chain fatty acids into fatty acyl-CoA esters. ACSL1 preferentially uses palmitoleate, oleate and linoleate, and altered expression of ACSL1 is thought to increase accumulation of triglycerides in the liver.

ALCS1 is strongly expressed in the heart, kindey, liver, skeletal muscle, and erythroid cells, and to a lesser extent in lung, brain, placenta and pancreas. This protein is predominantly expressed during the early stages of erythroid development, and expression is weak in reticulocytes and young erythrocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional ACSL1 Products

Array NBP1-89271

Research Areas for ACSL1 Antibody (NBP1-89271)

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Blogs on ACSL1.

Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.

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Bioinformatics

Gene Symbol ACSL1