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Images
Western Blot: Aconitase 2 Antibody (3G8) [NBP2-02241] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Western Blot: Aconitase 2 Antibody (3G8) [NBP2-02241] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunocytochemistry/ Immunofluorescence: Aconitase 2 Antibody (3G8) [NBP2-02241] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Aconitase 2.
Immunohistochemistry-Paraffin: Aconitase 2 Antibody (3G8) [NBP2-02241] - Staining of paraffin-embedded Ovary tissue using anti-Aconitase 2 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Aconitase 2 Antibody (3G8) [NBP2-02241] - Immunofluorescent staining of HepG2 cells using anti-Aconitase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Aconitase 2 Antibody (3G8) [NBP2-02241] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Aconitase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Aconitase 2 Antibody (3G8) [NBP2-02241] - Staining of paraffin-embedded Kidney tissue using anti-Aconitase 2 mouse monoclonal antibody.
85.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4 using NBP2-02241 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.9 mg/ml
Purity
Immunogen affinity purified
Alternate Names for Aconitase 2 Antibody (OTI3G8)
aconitase 2, mitochondrial
Aconitase
aconitate hydratase, mitochondrial
ACONM
Citrate hydro-lyase
EC 4.2.1
EC 4.2.1.3
MGC20605
MGC33908
Background
The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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