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Aconitase 2 Antibody (OTI3G8)

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Western Blot: Aconitase 2 Antibody (3G8) [NBP2-02241] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI3G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.9 mg/ml

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Reviews & Publications
Protocols & FAQs
Support & Research

Aconitase 2 Antibody (OTI3G8) Summary

Immunogen
Full length human recombinant protein of human ACO2 (NP_001089) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACO2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
Theoretical MW
85.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-02241 in the following applications:

Publications
Read Publication using NBP2-02241.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.9 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aconitase 2 Antibody (OTI3G8)

  • aconitase 2, mitochondrial
  • Aconitase
  • aconitate hydratase, mitochondrial
  • ACONM
  • Citrate hydro-lyase
  • EC 4.2.1
  • EC 4.2.1.3
  • MGC20605
  • MGC33908

Background

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Verified Customer
12/29/2014
Application: WB
Species: Rat

Bioinformatics

Gene Symbol ACO2
Entrez