Aconitase 2 Antibodies
We offer Aconitase 2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each Aconitase 2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Aconitase 2 Antibodies can be used in a variety of model species: Chicken, Human, Mouse, Porcine, Rat. Use the list below to choose the Aconitase 2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Aconitase 2 polyclonal antibodies and browse our Aconitase 2 monoclonal antibody catalog.
Alternate Names for Aconitase 2 Antibodies
anti-Aconitase 2 antibody, anti-ACO2 antibody, anti-aconitase 2, mitochondrial antibody, anti-Aconitase antibody, anti-aconitate hydratase, mitochondrial antibody, anti-ACONM antibody, anti-Citrate hydro-lyase antibody, anti-EC 4.2.1 antibody, anti-EC 4.2.1.3 antibody, anti-MGC20605 antibody, anti-MGC33908 antibody
12 Results for "aconitase-2" in Primary Antibodies
![Western Blot: Aconitase 2 Antibody [NBP1-32781] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [C1C3] diluted at 1:10000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-32781-img0007.jpg "Western Blot: Aconitase 2 Antibody [NBP1-32781] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [C1C3] diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody [NBP1-32781] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aconitase 2 protein stained by Aconitase 2 antibody [C1C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32781-img0009.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-32781] - Paraffin-embedded mouse duodenum. Aconitase 2 antibody [C1C3] dilution: 1:500.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32781-img0006.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-32781] - Sample (20 ug of whole cell lysate) A: mouse brain 7. 5% SDS PAGE, antibody diluted at 1:20000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-32781-img0004.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-32781] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE, antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-32781-img0005.jpg)
![Immunoprecipitation: Aconitase 2 Antibody [NBP1-32781] - HeLa whole cell extracts using 5 ug of Aconitase 2 antibody [C1C3]. Western blot analysis was performed using Aconitase 2 antibody [C1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunoprecipitation-NBP1-32781-img0011.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Staining in human heart muscle and liver tissues using NBP1-90264 antibody. Corresponding ACO2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90264-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Staining in human heart muscle and liver tissues using NBP1-90264 antibody. Corresponding ACO2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: Aconitase 2 Antibody [NBP1-90264] - STED super-resolution microscopy of mitochondria in the rectal Muscularis externa demonstrates high structural preservation of the stored paraffin-embedded tissue. STED recordings were performed on 2 um thick dewaxed sections cut along the longitudinal axis of the rectum. STED images of tissue sections decorated with antisera against aconitase. In each panel the confocal (left) and the corresponding STED image (right) is displayed. Magnification of the STED image as indicated by a dashed square. Scale bar: 200 nm. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0101563), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-NBP1-90264-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody [NBP1-90264] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90264-img0008.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-90264] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-90264-img0009.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-90264] - Analysis in human cell line HL-60.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-90264-img0006.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Staining of human duodenum shows strong strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90264-img0014.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90264-img0015.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Staining of human kidney shows strong strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90264-img0016.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-90264] - Staining of human liver shows very weak positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90264-img0017.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-90264] - The regulation mechanism of LOX1 on alveolar epithelial cell apoptosis & fibrosis. (a) Cells were transfected with a LOX1 overexpression plasmid & control vector. ROS levels were determined by DCFH-DA assay in MLE-12 cells treated by TGF-beta 1, TGF-beta 1 plus exosome, TGF-beta 1 plus exosome & vector, & TGF-beta 1 plus exosome & LOX1 overexpression plasmid (n = 3). (b) Detection of cellular mtDNA/18sRNA in each of the above cell groups (n = 3). (c) The expression of LOX1, caspase 3, mtDNA damage markers SIRT3 & ACO2, & NLRP3 was measured by western blotting in each of the above cell groups. (d) The expression of the same signal cascades including LOX1, caspase 3, SIRT3, ACO2, & NLRP3 was confirmed in an animal model with pulmonary fibrosis by western blot assay. Data is shown as the means ± SD, n ≥ 3. ∗∗p < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31949877), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-90264_rabbit-polyclonal-aconitase-2-antibody-310202416113522.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-90264] - The regulation mechanism of LOX1 on alveolar epithelial cell apoptosis & fibrosis. (a) Cells were transfected with a LOX1 overexpression plasmid & control vector. ROS levels were determined by DCFH-DA assay in MLE-12 cells treated by TGF-beta 1, TGF-beta 1 plus exosome, TGF-beta 1 plus exosome & vector, & TGF-beta 1 plus exosome & LOX1 overexpression plasmid (n = 3). (b) Detection of cellular mtDNA/18sRNA in each of the above cell groups (n = 3). (c) The expression of LOX1, caspase 3, mtDNA damage markers SIRT3 & ACO2, & NLRP3 was measured by western blotting in each of the above cell groups. (d) The expression of the same signal cascades including LOX1, caspase 3, SIRT3, ACO2, & NLRP3 was confirmed in an animal model with pulmonary fibrosis by western blot assay. Data is shown as the means ± SD, n ≥ 3. ∗∗p < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31949877), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-90264_rabbit-polyclonal-aconitase-2-antibody-310202416121946.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-90264] - The regulation mechanism of LOX1 on alveolar epithelial cell apoptosis & fibrosis. (a) Cells were transfected with a LOX1 overexpression plasmid & control vector. ROS levels were determined by DCFH-DA assay in MLE-12 cells treated by TGF-beta 1, TGF-beta 1 plus exosome, TGF-beta 1 plus exosome & vector, & TGF-beta 1 plus exosome & LOX1 overexpression plasmid (n = 3). (b) Detection of cellular mtDNA/18sRNA in each of the above cell groups (n = 3). (c) The expression of LOX1, caspase 3, mtDNA damage markers SIRT3 & ACO2, & NLRP3 was measured by western blotting in each of the above cell groups. (d) The expression of the same signal cascades including LOX1, caspase 3, SIRT3, ACO2, & NLRP3 was confirmed in an animal model with pulmonary fibrosis by western blot assay. Data is shown as the means ± SD, n ≥ 3. ∗∗p < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31949877), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-90264_rabbit-polyclonal-aconitase-2-antibody-31020241612192.jpg)
![Western Blot: Aconitase 2 Antibody (3G8) [NBP2-02241] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aconitase 2.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-3G8-Western-Blot-NBP2-02241-img0001.jpg "Western Blot: Aconitase 2 Antibody (3G8) [NBP2-02241] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aconitase 2.")
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody (3G8) [NBP2-02241] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Aconitase 2.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-3G8-Immunocytochemistry-Immunofluorescence-NBP2-02241-img0006.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody (3G8) [NBP2-02241] - Staining of paraffin-embedded Ovary tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-02241-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody (3G8) [NBP2-02241] - Immunofluorescent staining of HepG2 cells using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-3G8-Immunocytochemistry-Immunofluorescence-NBP2-02241-img0002.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody (3G8) [NBP2-02241] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-02241-img0003.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody (3G8) [NBP2-02241] - Staining of paraffin-embedded Kidney tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-02241-img0004.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [N1N3] diluted at 1:10000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP2-15245-img0012.jpg "Western Blot: Aconitase 2 Antibody [NBP2-15245] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [N1N3] diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody [NBP2-15245] - HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: Aconitase 2 protein stained by Aconitase 2 antibody [N1N3] diluted at 1:1000. Red: MitoTrackerR Red CMXRos, a mitochondria tracker. Blue: Hoechst 33342 staining.Scale bar = 10um](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15245-img0009.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse intestine. Aconitase 2 antibody [N1N3] dilution: 1:500.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15245-img0011.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP2-15245-img0003.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE Aconitase 2 antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP2-15245-img0004.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP2-15245-img0005.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse kidney. Aconitase 2 antibody [N1N3] dilution: 1:500.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15245-img0007.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse urinary bladder. Aconitase 2 antibody [N1N3] ) dilution: 1:500.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15245-img0008.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse ovary. Aconitase 2 antibody [N1N3] dilution: 1:500](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15245-img0010.jpg)
![Immunoprecipitation: Aconitase 2 Antibody [NBP2-15245] - Immunoprecipitation of Aconitase 2 protein from HeLa whole cell extracts using 5 ug of Aconitase 2 antibody [N1N3] . Western blot analysis was performed using Aconitase 2 antibody [N1N3] .EasyBlot anti-Rabbit IgG was used as a secondary reagent](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunoprecipitation-NBP2-15245-img0006.jpg)
![Immunohistochemistry: Aconitase 2 Antibody [NBP2-15245] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Aconitase 2 antibody [N1N3] (NBP2-15245) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-15245_rabbit-polyclonal-aconitase-2-antibody-910202420134052.jpg)
![Western Blot: Aconitase 2 Antibody [NBP2-15245] - Sample (30 ug of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE NBP2-15245 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-15245_rabbit-polyclonal-aconitase-2-antibody-9102024205635.jpg)
![Western Blot: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aconitase](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Western-Blot-NBP2-70074-img0001.jpg "Western Blot: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aconitase")
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Aconitase 2.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70074-img0003.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Kidney tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70074-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of HepG2 cells using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70074-img0002.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70074-img0004.jpg)
![Immunohistochemistry: Aconitase 2 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70074] - Staining of paraffin-embedded Ovary tissue using anti-Aconitase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70074-img0006.jpg)
![Western Blot: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Western blot analysis of extracts of various cell lines, using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-0P3H3-Western-Blot-NBP3-16426-img0005.jpg "Western Blot: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Western blot analysis of extracts of various cell lines, using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunofluorescence analysis of NIH-3T3 cells using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-0P3H3-Immunocytochemistry-Immunofluorescence-NBP3-16426-img0004.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunohistochemistry of paraffin-embedded mouse kidney using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-0P3H3-Immunohistochemistry-Paraffin-NBP3-16426-img0003.jpg)
![Western Blot: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Western blot analysis of extracts of various cell lines, using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-0P3H3-Western-Blot-NBP3-16426-img0006.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunohistochemistry of paraffin-embedded rat kidney using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-0P3H3-Immunohistochemistry-Paraffin-NBP3-16426-img0001.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody (0P3H3) [NBP3-16426] - Immunohistochemistry of paraffin-embedded human colon using Aconitase 2 (ACO2) Rabbit mAb (NBP3-16426) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-0P3H3-Immunohistochemistry-Paraffin-NBP3-16426-img0002.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-52015] - Analysis of Mouse (A), Rat (B) and Pig (C) Skeletal Muscle lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-52015-img0005.jpg "Western Blot: Aconitase 2 Antibody [NBP1-52015] - Analysis of Mouse (A), Rat (B) and Pig (C) Skeletal Muscle lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-52015] - (3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-52015-img0002.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-52015] - Analysis of Aconitase 2 (aa541-555) in Heart lysate (35ug protein in RIPA buffer) using this antibody at 0.01ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-52015-img0003.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-52015] - Analysis of Human (A), Mouse (B) and Rat (C) Adipose lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-52015-img0004.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-52014] - (0.01ug/ml) staining of Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-52014-img0002.jpg "Western Blot: Aconitase 2 Antibody [NBP1-52014] - (0.01ug/ml) staining of Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-52014] - (3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-52014-img0003.jpg)
![Western Blot: Aconitase 2 Antibody (S07-7A1) [NBP3-19829] - Western blot detection of Aconitase 2 in Hela, A549, C6 using NBP3-19829 (1:1000 diluted)](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-S07-7A1-Western-Blot-NBP3-19829-img0002.jpg "Western Blot: Aconitase 2 Antibody (S07-7A1) [NBP3-19829] - Western blot detection of Aconitase 2 in Hela, A549, C6 using NBP3-19829 (1:1000 diluted)")
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody (S07-7A1) [NBP3-19829] - Immunohistochemical of Aconitase 2 in Human breast cancer tissue using NBP3-19829 at dilution 1/50](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-S07-7A1-Immunohistochemistry-Paraffin-NBP3-19829-img0001.jpg)
![Immunoprecipitation:Aconitase 2 AntibodyNBP3-29844] -Detection of human ACO2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACO2 antibody NBP3-29844 (lot NBP3-29844-1) used for IP at 6 µg per reaction. ACO2 was also immunoprecipitated by rabbit anti-ACO2.. For blotting immunoprecipitated ACO2, NBP3-29844 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29844_rabbit-aconitase-2-pab-301220241556519.jpg "Immunoprecipitation:Aconitase 2 AntibodyNBP3-29844] -Detection of human ACO2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACO2 antibody NBP3-29844 (lot NBP3-29844-1) used for IP at 6 µg per reaction. ACO2 was also immunoprecipitated by rabbit anti-ACO2.. For blotting immunoprecipitated ACO2, NBP3-29844 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation:Aconitase 2 AntibodyNBP3-29659] -Detection of human ACO2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACO2 antibody NBP3-29659 (lot NBP3-29659-1) used for IP at 6 µg per reaction. ACO2 was also immunoprecipitated by rabbit anti-ACO2.. For blotting immunoprecipitated ACO2, NBP3-29659 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-29659_rabbit-aconitase-2-pab-301220241321393.jpg "Immunoprecipitation:Aconitase 2 AntibodyNBP3-29659] -Detection of human ACO2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACO2 antibody NBP3-29659 (lot NBP3-29659-1) used for IP at 6 µg per reaction. ACO2 was also immunoprecipitated by rabbit anti-ACO2.. For blotting immunoprecipitated ACO2, NBP3-29659 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunohistochemistry: Aconitase 2 Antibody (8H4) [NBP3-26528] - Image of Aconitase 2 Antibody (8H4) diluted at 1:100 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26528_rabbit-aconitase-2-mab-8h4-26220241447159.jpg "Immunohistochemistry: Aconitase 2 Antibody (8H4) [NBP3-26528] - Image of Aconitase 2 Antibody (8H4) diluted at 1:100 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Aconitase 2 Antibody (8H4) [NBP3-26528] - Image of Aconitase 2 Antibody (8H4) diluted at 1:100 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26528_rabbit-aconitase-2-mab-8h4-26220241522427.jpg)