ACE-2 Antibody (SN0754)

Angiotensin I Converting Enzyme 2 (ACE2), also known as ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family (theoretical molecular weight 92 kDa). The predicted mouse ACE2 protein sequence consists of 798 amino acids, including an N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. Mouse ACE2 is 40% homologous in amino acid identity to the N- and C-terminal domains of mouse somatic ACE. Enzymatically, ACE2 converts the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts Ang I to Ang II.

Genetic data from Drosophila, mice and rats show that the ACE2 protein is an essential regulator of heart function in vivo. ACE2 mRNA is found at high levels in testis, kidney and heart with moderate levels in colon, small intestine, and ovary. The ACE2 protein contributes to organ function through the renin-angiotensin system, playing a central role in vascular, renal, and myocardial physiology.

Virology research has demonstrated that the severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein binds to its functional receptor, ACE2 (1). Vimentin is a type III intermediate filament protein expressed in mesenchymal cells that helps comprise the cytoskeleton in all animal cells. Studies have demonstrated that vimentin allows cell binding that allows the uptake of SARS-CoV spike protein into a host (2). This direct interaction of SARS-CoV with vimentin has been identified as an entry mechanism for the virus into a host, mediated by the SARS-CoV receptor ACE2 (1,2). It has been shown that ACE2 is the SARS-CoV-2 receptor required for cell entry and plays a physiological role in the replication of SARS-CoV in an infected host (1). Studies using human ACE2 antibody demonstrated a blockade of SARS-CoV-2 and ACE2 interaction, indicating an important physiological component of viral transmission and potential anti-viral therapeutic strategies (3).

References

1. Li, W., Moore, M. J., Vasilieva, N., Sui, J., Wong, S. K., Berne, M. A., . . . Farzan, M. (2003). Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature, 426(6965), 450-454. doi:10.1038/nature02145

2. Yu YT, Chien SC, Chen IY, Lai CT, Tsay YG, Chang SC, Chang MF. (2016) Surface vimentin is critical for the cell entry of SARS-CoV. J Biomed Sci. doi: 10.1186/s12929-016-0234-7

3. Hoffmann, M., Kleine-Weber, H., Schroeder, S., Kruger, N., Herrler, T., Erichsen, S., . . . Pohlmann, S. (2020). SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. doi:10.1016/j.cell.2020.02.052

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Monkey.

We have publications tested in 7 applications: ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IP, WB.

Showing Publications 1 - 10 of 21. Show All 21 Publications. Collapse Publications.

Publications using NBP2-67692	Applications	Species
Subramaniyan B, Gurung S, Bodas M et al. The Isolation and In Vitro Differentiation of Primary Fetal Baboon Tracheal Epithelial Cells for the Study of SARS-CoV-2 Host-Virus Interactions Viruses 2023-03-28 [PMID: 37112842] (WB, Monkey) Details: 1:1000 WB dilution	WB	Monkey
Clark J, Renrice-Randal R, Sharma P et al. Sequential infection with influenza A virus followed by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) leads to more severe disease and encephalitis in a mouse model of COVID-19 bioRXiv 2020-10-13 (IHC-P, Mouse)	IHC-P	Mouse
Helms L Utility of kidney organoids for disease modeling and therapeutic development Thesis 2022-01-01 (IHC-P, Mouse)	IHC-P	Mouse
Tiwari S, Rana T Generation of 3D lung organoids from human induced pluripotent stem cells for modeling of lung development and viral infection Heliyon 2023-09-01 (IHC, WB, Human)	IHC, WB	Human
Lindqvist R, Benz C, Sereikaite V et al. A Syntenin Inhibitor Blocks Endosomal Entry of SARS-CoV-2 and a Panel of RNA Viruses Viruses 2022-10-06 [PMID: 36298757] (ICC/IF, Human) Details: Dilution used in ICC 1:500	ICC/IF	Human
Ireland RE, Davies CD, Keyser E et al. Histopathological and Immunological Findings in the Common Marmoset Following Exposure to Aerosolized SARS-CoV-2 Viruses 2022-07-21 [PMID: 35891560] (IHC-Fr)	IHC-Fr
Xue Y, Yang D, Vogel P et al. Cardiopulmonary Injury in the Syrian Hamster Model of COVID-19 Viruses 2022-06-27 [PMID: 35891384]
Lee RK, Li TN, Chang SY et al. Identification of Entry Inhibitors against Delta and Omicron Variants of SARS-CoV-2 International journal of molecular sciences 2022-04-06 [PMID: 35409412] (ICC/IF, Human)	ICC/IF	Human
Ramal-Sanchez M, Castellini C, Cimini C et al. ACE2 Receptor and Its Isoform Short-ACE2 Are Expressed on Human Spermatozoa International journal of molecular sciences 2022-03-28 [PMID: 35409054] (WB, IF/IHC, Human)	WB, IF/IHC	Human
Zeng FM, Li YW, Deng ZH et al. SARS-CoV-2 spike spurs intestinal inflammation via VEGF production in enterocytes EMBO molecular medicine 2022-03-31 [PMID: 35362189] (WB, Mouse)	WB	Mouse
T Yamaguchi, M Hoshizaki, T Minato, S Nirasawa, MN Asaka, M Niiyama, M Imai, A Uda, JF Chan, S Takahashi, J An, A Saku, R Nukiwa, D Utsumi, M Kiso, A Yasuhara, VK Poon, CC Chan, Y Fujino, S Motoyama, S Nagata, JM Penninger, H Kamada, KY Yuen, W Kamitani, K Maeda, Y Kawaoka, Y Yasutomi, Y Imai, K Kuba ACE2-like carboxypeptidase B38-CAP protects from SARS-CoV-2-induced lung injury Nature Communications, 2021-11-23;12(1):6791. 2021-11-23 [PMID: 34815389]
Tu WJ, McCuaig RD, Melino M et al. Targeting novel LSD1-dependent ACE2 demethylation domains inhibits SARS-CoV-2 replication Cell discovery 2021-05-24 [PMID: 34031383] (IP, Human)	IP	Human
Blume C, Jacskson C, Spalluto C et al. A novel isoform of ACE2 is expressed in human nasal and bronchial respiratory epithelia and is upregulated in response to RNA respiratory virus infection bioRXiv Jul 31 2020 (ICC/IF, Human)	ICC/IF	Human
Forsyth CB, Zhang L, Bhushan A et al. The SARS-CoV-2 S1 Spike Protein Promotes MAPK and NF-kB Activation in Human Lung Cells and Inflammatory Cytokine Production in Human Lung and Intestinal Epithelial Cells Microorganisms 2022-10-10 [PMID: 36296272] (WB, Human)	WB	Human
Stanic B, Maddox S, Arlindo de Souza AM et al. Male bias in ACE2 basic science research: Missed opportunity for discovery in the time of COVID-19 American journal of physiology. Regulatory, integrative and comparative physiology 2021-04-13 [PMID: 33848207] (WB, Mouse)	WB	Mouse
Joyraj Bhattacharjee M, Lin JJ, Chang CY et al. Identifying primate ACE2 variants that confer resistance to SARS-CoV-2 Molecular biology and evolution 2021-03-01 [PMID: 33674876] (WB, Human)	WB	Human
Suresh V, Parida D, Minz A, Senapati S Tissue distribution of ACE2 protein in Syrian golden hamster (Mesocricetus auratus) and its possible implications in SARS-CoV-2 related studies Front Pharmacol 2021-02-11 [PMID: 33568991]
Blume C, Jackson CL, Spalluto CM, et al. A novel ACE2 isoform is expressed in human respiratory epithelia and is upregulated in response to interferons and RNA respiratory virus infection Nature genetics 2021-01-11 [PMID: 33432184] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Lee IT, Nakayama T, Wu CT et al ACE2 localizes to the respiratory cilia and is not increased by ACE inhibitors or ARBs Nat Commun 2020-10-29 [PMID: 33116139] (IHC-P, IHC-P, Human) Details: Citation using the Azide and BSA Free version of this antibody.	IHC-P, IHC-P	Human
Kalidhindi RSR, Borkar NA, Ambhore NS et al. Sex steroids skew ACE2 expression in human airway: a contributing factor to sex differences in COVID-19? Am J Physiol Lung Cell Mol Physiol 2020-11-01 [PMID: 32996784] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Lee IT, Nakayama T, Wu CT et al. Robust ACE2 protein expression localizes to the motile cilia of the respiratory tract epithelia and is not increased by ACE inhibitors or angiotensin receptor blockers medRxiv 2020-01-01 [PMID: 32511516] (IHC-P, Human) Details: Citation using the Azide and BSA Free format of this antibody.	IHC-P	Human
Show All 21 Publications. Collapse Publications.

Array NBP2-67692

• ACE-2 Antibodies
• ACE-2 Antibody Pair
• ACE-2 ELISA Kits
• ACE-2 Kit
• ACE-2 Proteins
• ACE-2 Proteins and Enzymes
• ACE-2 ELISAs