![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-46140] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp1-46140_goat-polyclonal-acat1-antibody-imgenex-img-31254-16520239183319.jpg "Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-46140] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
| Reactivity | Hu, Mu, Rt, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-DEEYKRVDFSKVPK corresponding to internal region according to NP_000010.1. |
| Predicted Species | Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | ACAT1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 40kDa band observed in lysates of cell lines CAC02, HEK293, HepG2, MCF7, NIH3T3 and KNRK and approx. 38-40kDa in Human, Mouse and Rat Liver lysates (calculated MW of 45.2kDa according to Human NP_000010.1, 44.81kDa according to Mouse NP_659033.1 and 44.7kDa according to Rat NP_058771.2 ). Primary incubation 1 hour at room temperature. |
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| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NBP1-46140 | Applications | Species |
|---|---|---|
| Hongo S, Watanabe T, Arita S et al. Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. Am J Physiol Cell Physiol 2009-08-01 [PMID: 19625677] |
Secondary Antibodies |
Isotype Controls |
Research Areas for ACAT1 Antibody (NBP1-46140)Find related products by research area.
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![Western Blot: ACAT1 Antibody [NBP1-46140] - (0.01ug/ml) staining of Human (A), and (0.1ug/ml) Mouse (B) and Rat (C), Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp1-46140_goat-polyclonal-acat1-antibody-imgenex-img-31254-16520239183320.jpg "Western Blot: ACAT1 Antibody [NBP1-46140] - (0.01ug/ml) staining of Human (A), and (0.1ug/ml) Mouse (B) and Rat (C), Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: ACAT1 Antibody [NBP1-46140] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp1-46140_goat-polyclonal-acat1-antibody-imgenex-img-31254-16520239183336.jpg "Flow Cytometry: ACAT1 Antibody [NBP1-46140] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: ACAT1 Antibody [NBP1-46140] - (0.5ug/ml) staining of KNRK (A) and MCF7 (B), and (0.1ug/ml) HepG2 (C), Caco-2 (D), HEK293 (E), and NIH3T3 (F) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp1-46140_goat-polyclonal-acat1-antibody-imgenex-img-31254-16520239183337.jpg "Western Blot: ACAT1 Antibody [NBP1-46140] - (0.5ug/ml) staining of KNRK (A) and MCF7 (B), and (0.1ug/ml) HepG2 (C), Caco-2 (D), HEK293 (E), and NIH3T3 (F) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
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