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ACAT1 Antibody (2C5) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
2C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700

Order Details

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ACAT1 Antibody (2C5) [Alexa Fluor® 700] Summary

Immunogen
Recombinant human ACAT1 (34-427aa) purified from E. coli
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACAT1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ACAT1 Antibody (2C5) [Alexa Fluor® 700]

  • ACAT
  • ACAT1
  • acetoacetyl Coenzyme A thiolase
  • Acetoacetyl-CoA thiolase
  • acetyl-CoA acetyltransferase 1
  • acetyl-CoA acetyltransferase, mitochondrial
  • acetyl-Coenzyme A acetyltransferase 1
  • EC 2.3.1
  • EC 2.3.1.9
  • MAT
  • mitochondrial acetoacetyl-CoA thiolase
  • T2
  • THIL

Background

Sterol O-acyltransferase 1 (SOAT1), also known as ACAT1, catalyzes the formation of intracellular cholesterol esters in various tissues, such as adult human liver, adrenal glands, macrophages, and kidneys. Specifically, ACAT-1 catalyzes the reversible reaction forming acetoacetyl-CoA from two molecules of acetyl-CoA. Additionaly, the ACAT 1 protein may have a role in lipoprotein assembly and dietary cholesterol absorption, and may also act as a ligase.

Mutations in this gene have been associated with 3-ketothiolase deficiency, an inborn defect of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACAT1