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5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

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5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric) [NBP3-24452] - Standard Reference Curve
5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric) [NBP3-24452] - Standard Reference Curve
5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric) [NBP3-24452] - Assay Example

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Func
Suitable Sample Type
Serum, plasma and animal tissue
Standard Curve Range
28.0 - 581 U/L
Sensitivity
28.0 U/L

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5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric) Summary

Description
Detection instrument: Microplate reader (550 nm)
Assay time: 30 min
Standard Curve Range
28.0 - 581 U/L
Sensitivity
28.0 U/L
Inter-Assay
%CV < 6
Intra-Assay
%CV < 3.3
Spike Recovery
Average recovery rate: 105%
Kit Type
Activity Assay Kit (Colorimetric)
Gene
NT5E

Applications/Dilutions

Dilutions
  • Functional

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Microplate
  2. Plate Sealer
  3. Reagent 1: Working Solution
  4. Reagent 2: Chromogenic Agent
  5. Reagent 3: 0.6 mmol/L Inosine Standard Solution

Alternate Names for 5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

  • 5' nucleotidase (CD73)
  • 5'-NT
  • 5-NT
  • 5'-Nucleotidase
  • 5'-nucleotidase, ecto (CD73)
  • CD73 antigen
  • CD73
  • E5NT
  • EC 3.1.3.5
  • ecto-5'-nucleotidase
  • eN
  • eNT
  • NT
  • NT55'-nucleotidase
  • NT5E
  • NTE
  • Purine 5-Prime-Nucleotidase

Background

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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FAQs for 5'-Nucleotidase/CD73 Kit (NBP3-24452). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

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  Manual - 5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)
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Bioinformatics

Gene Symbol NT5E