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Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence)

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ELISA: Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence) [NBP2-66370] - Samples were spiked with high concentrations of Human 5'-Nucleotidase/CD73 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence) [NBP2-66370] - Samples were spiked with high concentrations of Human 5'-Nucleotidase/CD73 and diluted with Reference Standard & Sample Diluent to produce ...read more
ELISA: Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence) [NBP2-66370] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
62.50-4000 pg/mL
Sensitivity
37.50 pg/mL

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Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Human 5-NT in samples. No significant cross-reactivity or interference between Human 5-NT and analogues was observed.
Standard Curve Range
62.50-4000 pg/mL
Sensitivity
37.50 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 9.13%
Intra-Assay
CV% < 9.1%
Spike Recovery
85-107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
NT5E

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence)

  • 5' nucleotidase (CD73)
  • 5'-NT
  • 5-NT
  • 5'-Nucleotidase
  • 5'-nucleotidase, ecto (CD73)
  • CD73 antigen
  • CD73
  • E5NT
  • EC 3.1.3.5
  • ecto-5'-nucleotidase
  • eN
  • eNT
  • NT
  • NT55'-nucleotidase
  • NT5E
  • NTE
  • Purine 5-Prime-Nucleotidase

Background

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for 5'-Nucleotidase/CD73 ELISA Kit (NBP2-66370). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

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Product PDFs

  Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol NT5E