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5-HT2A Antibody (1055220) [Unconjugated]

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5-HT2A was detected in immersion fixed NS0 mouse myeloma cell line transfected (positive) cells and absent in NS0 mouse myeloma cell line wild type (negative) cells using Mouse Anti-Human 5-HT2A Monoclonal Antibody ...read more
HEK293 cells transfected with Human HT2A and eGFP vs irrelevant were stained with eGFP and Mouse Anti-Human 5-HT2A Monoclonal Antibody (A, B) (Catalog # MAB112501) followed by Allophycocyanin-conjugated Anti-Mouse IgG ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF
Clone
1055220
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

5-HT2A Antibody (1055220) [Unconjugated] Summary

Immunogen
Synthetic peptide corresponding to the amino acids 1-75 in the N-term extracellular domain of the Serotonin Receptor 5-HT2A (HTR2A).
Accession # P28223
Specificity
Detects human HTR2A in direct ELISA.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 5-HT2A Antibody (1055220) [Unconjugated]

  • 5-HT2 receptor
  • 5-HT-2
  • 5HT2A
  • 5-HT2A
  • 5-HT-2A
  • 5-hydroxytryptamine (serotonin) receptor 2A
  • 5-hydroxytryptamine receptor 2A
  • HTR2,5-HT2A
  • HTR2A
  • serotonin 5-HT-2A receptor
  • Serotonin receptor 2A

Background

Human serotonin receptor HTR2A, also known as 5-HT2A or HTR2, is a G-protein coupled receptor (GPCR) for 5-hydroxytryptamine (serotonin). The 5-HT2A receptor is mainly a cell surface receptor with several intracellular locations. Human -5-HT2A is the main excitatory receptor subtype among the GPCRs for serotonin but an inhibitory effect on the visual cortex and the orbitofrontal cortex has also been described. This receptor was first noted for its importance as a target of serotonergic psychedelic drugs such as LSD and psilocybin mushrooms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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