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14-3-3 tau/theta Antibody

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Western Blot: 14-3-3 tau/theta Antibody [NBP1-06008] - (0.03ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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14-3-3 tau/theta Antibody Summary

Immunogen
Peptide with sequence C-DDRKQTIDNSQ corresponding to internal region according to NP_006817.1.
Epitope
C-DDRKQTIDNSQ
Predicted Species
Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
YWHAQ
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 28 kDa band observed in human brain (hippocampus) lysates (calculated MW of 27.8 kDa band according to NP_006817.1).
Control
14-3-3 tau/theta Overexpression Lysate
Publications
Read Publication using NBP1-06008.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 14-3-3 tau/theta Antibody

  • 14-3-3 protein tau
  • 14-3-3 protein T-cell
  • 14-3-3 protein theta
  • 1433 theta
  • 14-3-3 theta
  • 14-3-3,1C5
  • HS1 protein
  • HS1
  • Protein HS1
  • protein tau
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, thetapolypeptide
  • YWHAQ

Background

14-3-3 theta belongs to the 14-3-3 family of proteins that mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and it is 99% identical to the mouse and rat orthologs. 14-3-3 theta is upregulated in patients with amyotrophic lateral sclerosis (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 14-3-3 tau/theta Antibody (NBP1-06008)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for 14-3-3 tau/theta Antibody (NBP1-06008) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol YWHAQ
Entrez
Uniprot