![Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) - Azide and BSA Free [NBP2-79944] - Western Blot Analysis of HEK293, PC-3 and A431 cell lysates using 14-3-3E Mouse Monoclonal Antibody (CPTC-YWHAE-1).](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-CPTC-YWHAE-1-Azide-and-BSA-Free-Western-Blot-NBP2-79944-img0002.jpg "Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) - Azide and BSA Free [NBP2-79944] - Western Blot Analysis of HEK293, PC-3 and A431 cell lysates using 14-3-3E Mouse Monoclonal Antibody (CPTC-YWHAE-1).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, MA |
| Clone | CPTC-YWHAE-1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79810). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human full-length 14-3-3 epsilon protein (Uniprot: P62268) |
| Localization | Cytoplasm, Melanosome |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | YWHAE |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 29.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for 14-3-3 epsilon Antibody (NBP2-79944)Find related products by research area.
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| Gene Symbol | YWHAE |
![Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) - Azide and BSA Free [NBP2-79944] - Formalin-fixed, paraffin-embedded human Testis stained with 14-3-3E Mouse Monoclonal Antibody (CPTC-YWHAE-1).](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-CPTC-YWHAE-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79944-img0001.jpg "Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) - Azide and BSA Free [NBP2-79944] - Formalin-fixed, paraffin-embedded human Testis stained with 14-3-3E Mouse Monoclonal Antibody (CPTC-YWHAE-1).")
![Protein Array: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) - Azide and BSA Free [NBP2-79944] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-CPTC-YWHAE-1-Azide-and-BSA-Free-Protein-Array-NBP2-79944-img0003.jpg "Protein Array: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) - Azide and BSA Free [NBP2-79944] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.")
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