I need to know about TNF-alpha antibody kits for mice.

We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.

I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.

I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.

I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?

The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.

Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?

I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

TNF-alpha Antibody (1825) [Unconjugated] (MAB210): Novus Biologicals

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	B/N
Clone	1825
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Formulation	Size	Price
Carrier Free MAB210-SP

Carrier Free MAB210-500

Immunogen	E. coli-derived recombinant human TNF-alpha Gly57-Leu233 Accession # P01375
Specificity	Detects human TNF-alpha in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant rhesus macaque TNF-alpha is observed and no cross-reactivity with recombinant cotton rat, mouse, rat, or porcine TNF-alpha is observed. It shows no cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhEDA‑A2, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhOX40 Ligand, rhRANK Ligand, rhTNF-beta , rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI.
Source	N/A
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	TNF
Purity Statement	Protein A or G purified from ascites
Endotoxin Note	<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.5 mg/mL in sterile PBS.

TNF-alpha Antibody (1825) [Unconjugated]

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TNF-alpha Antibody (1825) [Unconjugated] Summary

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Publications for TNF-alpha Antibody (MAB210)(30)

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FAQs for TNF-alpha Antibody (MAB210). (Showing 1 - 4 of 4 FAQs).

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Bioinformatics

Publications using MAB210	Applications	Species
Bhuyan S, Pal B, Pathak L, Saikia P, Mitra S, Gayan S, Mokhtari R, Li H, Ramana C, Baishya D, Das B Targeting hypoxia-induced tumor stemness by activating pathogen-induced stem cell niche defense. Front Immunol, 2022-09-29;13(0):933329. 2022-09-29 [PMID: 36248858] (Neutralization, Complex Species Category)	Neutralization	Complex Species Category
Hoai-Nga Thi Nguyen, Marie Kawahara, Cat-Khanh Vuong, Mizuho Fukushige, Toshiharu Yamashita, Osamu Ohneda SARS-CoV-2 M Protein Facilitates Malignant Transformation of Breast Cancer Cells Frontiers in Oncology 2022-06-07 [PMID: 35747796]
Chao Gu, Katherine Upchurch, Joshua Horton, Mathew Wiest, Sandra Zurawski, Mark Millard et al. Dectin-1 Controls TSLP-Induced Th2 Response by Regulating STAT3, STAT6, and p50-RelB Activities in Dendritic Cells Frontiers in Immunology 7/7/2021 [PMID: 34305908]
Jungnam Lee, Yuanqing Lu, Regina Oshins, Jesse West, Craig G. Moneypenny, Kyudong Han et al. Alpha 1 Antitrypsin-Deficient Macrophages Have Impaired Efferocytosis of Apoptotic Neutrophils Frontiers in Immunology 2020-11-20 [PMID: 33329539]
JH Kim, JY Kim, M Park, S Kim, T Kim, J Kim, S Choi, W Park, JY Hwang, J Choe, KS Ha, MH Won, S Ryoo, YG Kwon, YM Kim NF-kappaB-dependent miR-31/155 biogenesis is essential for TNF-alpha-induced impairment of endothelial progenitor cell function Exp. Mol. Med., 2020-08-07;0(0):. 2020-08-07 [PMID: 32770080] (Neutralization, Human)	Neutralization	Human
J Sellau, M Groneberg, H Fehling, T Thye, S Hoenow, C Marggraff, M Weskamm, C Hansen, S Stanelle-B, S Kuehl, J Noll, V Wolf, NG Metwally, SH Hagen, C Dorn, J Wernecke, H Ittrich, E Tannich, T Jacobs, I Bruchhaus, M Altfeld, H Lotter Androgens predispose males to monocyte-mediated immunopathology by inducing the expression of leukocyte recruitment factor CXCL1 Nat Commun, 2020-07-10;11(1):3459. 2020-07-10 [PMID: 32651360] (Neutralization, Human)	Neutralization	Human
Małgorzata Pawlikowska, Tomasz Jędrzejewski, Anna A Brożyna, Sylwia Wrotek Protein-Bound Polysaccharides from Coriolus Versicolor Induce RIPK1/RIPK3/MLKL-Mediated Necroptosis in ER-Positive Breast Cancer and Amelanotic Melanoma Cells Cellular Physiology and Biochemistry 2020-06-13 [PMID: 32531147]
Rhee HJ, Shaib AH, Rehbach K et al. Pro-Inflammatory Activation of A New Immortalized Human Microglia Cell Line Brain Sci 5/15/2019 [PMID: 31096716]
Cassetta L, Fragkogianni S, Sims AH et al. Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets. Cancer Cell. 4/15/2019 [PMID: 30930117]
Hongxiao Song, Guangyun Tan, Yang Yang, An Cui, Haijun Li, Tianyang Li et al. Hepatitis B Virus–Induced Imbalance of Inflammatory and Antiviral Signaling by Differential Phosphorylation of STAT1 in Human Monocytes The Journal of Immunology 2019-04-15 [PMID: 30842274]
Xiulei Mo, Cong Tang, Qiankun Niu, Tingxuan Ma, Yuhong Du, Haian Fu HTiP: High-Throughput Immunomodulator Phenotypic Screening Platform to Reveal IAP Antagonists as Anti-cancer Immune Enhancers Cell Chemical Biology 3/1/2019 [PMID: 30639259]
AK Reuschl, MR Edwards, R Parker, DW Connell, L Hoang, A Halliday, H Jarvis, N Siddiqui, C Wright, S Bremang, SM Newton, P Beverley, RJ Shattock, OM Kon, A Lalvani Innate activation of human primary epithelial cells broadens the host response to Mycobacterium tuberculosis in the airways PLoS Pathog., 2017-09-01;13(9):e1006577. 2017-09-01 [PMID: 28863187] (Neutralization, Human)	Neutralization	Human
Lijie Zhao, Tingli Sun, Lina Wang Chitosan oligosaccharide improves the therapeutic efficacy of sitagliptin for the therapy of Chinese elderly patients with type 2 diabetes mellitus Therapeutics and Clinical Risk Management 7/20/2017 [PMID: 28721055]
J Uebele, C Stein, MT Nguyen, A Schneider, F Kleinert, O Tichá, G Bierbaum, F Götz, I Bekeredjia Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus PLoS Pathog., 2017-05-25;13(5):e1006387. 2017-05-25 [PMID: 28542586] (Neutralization, Human)	Neutralization	Human
Killian Hurley, Noreen Lacey, Ciara A. O’Dwyer, David A. Bergin, Oliver J. McElvaney, M. Emmet O’Brien et al. Alpha-1 Antitrypsin Augmentation Therapy Corrects Accelerated Neutrophil Apoptosis in Deficient Individuals The Journal of Immunology 2014-10-15 [PMID: 25217166]
Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells. J Immunol, 2014-04-30;192(11):5332-42. 2014-04-30 [PMID: 24790151] (Neutralization, Human)	Neutralization	Human
Seeger P, Bosisio D, Parolini S, Badolato R, Gismondi A, Santoni A, Sozzani S Activin A as a mediator of NK-dendritic cell functional interactions. J Immunol, 2014-01-06;192(3):1241-8. 2014-01-06 [PMID: 24395917] (Neutralization, Human)	Neutralization	Human
Whilding LM, Archibald KM, Kulbe H et al. Vaccinia virus induces programmed necrosis in ovarian cancer cells. Mol Ther 11/1/2013 [PMID: 23985697]
Chih-Sheng Chu, Yu-Chen Wang, Long-Sheng Lu, Brian Walton, H. Ramazan Yilmaz, Roger Y. Huang et al. Electronegative Low-Density Lipoprotein Increases C-Reactive Protein Expression in Vascular Endothelial Cells through the LOX-1 Receptor PLoS ONE 2013-08-08 [PMID: 23950953]
Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation. PLoS Biol, 2012-09-25;10(9):e1001395. 2012-09-25 [PMID: 23055831] (Neutralization, Human)	Neutralization	Human
Nicolaidou V, Wong MM, Redpath AN Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation. PLoS ONE, 2012-07-03;7(7):e39871. 2012-07-03 [PMID: 22802946] (Neutralization, Human)	Neutralization	Human
Stephen H.-F. Macdonald, Elliott Woodward, Michelle M. Coleman, Emma R. Dorris, Parthiban Nadarajan, Wui-Mei Chew et al. Networked T Cell Death following Macrophage Infection by Mycobacterium tuberculosis PLoS ONE 2012-06-04 [PMID: 22675566]
Niklas Hagberg, Olof Berggren, Dag Leonard, Gert Weber, Yenan T. Bryceson, Gunnar V. Alm et al. IFN-alpha Production by Plasmacytoid Dendritic Cells Stimulated with RNA-Containing Immune Complexes Is Promoted by NK Cells via MIP-1 beta and LFA-1 The Journal of Immunology 2011-05-01 [PMID: 21430220]
Navenot JM, Fujii N, Peiper SC KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis. Mol. Pharmacol., 2009-02-06;75(5):1074-83. 2009-02-06 [PMID: 19201817] (Neutralization, Human)	Neutralization	Human
Arnold R, Neumann M, Konig W Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells. Immunology, 2007-05-01;121(1):71-81. 2007-05-01 [PMID: 17425601] (Neutralization, Human)	Neutralization	Human
van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers. Dig. Dis. Sci., 2007-03-30;52(10):2775-83. 2007-03-30 [PMID: 17394066] (Neutralization, Human)	Neutralization	Human
Pontikoglou C, Liapakis G, Pyrovolaki K, Papadakis M, Bux J, Eliopoulos GD, Papadaki HA Evidence for downregulation of erythropoietin receptor in bone marrow erythroid cells of patients with chronic idiopathic neutropenia. Exp. Hematol., 2006-10-01;34(10):1312-22. 2006-10-01 [PMID: 16982324] (Neutralization, Human)	Neutralization	Human
Ebert EC, Mehta V Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Cell. Immunol., 2006-08-17;241(1):7-13. 2006-08-17 [PMID: 16914126] (Neutralization, Human)	Neutralization	Human
Tay SS, McCormack A, Rose ML Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production. Transplantation, 2004-10-15;78(7):987-94. 2004-10-15 [PMID: 15480163] (Neutralization, Human)	Neutralization	Human
Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases. J. Leukoc. Biol., 2001-10-01;70(4):617-23. 2001-10-01 [PMID: 11590199] (Neutralization, Human)	Neutralization	Human
Show All 30 Publications. Collapse Publications.

Gene Symbol	TNF
Uniprot	P01375()