MYF-5 Antibody (OTI2G5) Summary
| Immunogen |
Full length human recombinant protein of human MYF5 (NP_005584) produced in E.coli. |
| Isotype |
IgG1 |
| Clonality |
Monoclonal |
| Host |
Mouse |
| Gene |
MYF5 |
| Purity |
Immunogen affinity purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Immunohistochemistry 1:150
- Immunohistochemistry-Paraffin 1:150
- Western Blot 1:2000
|
| Theoretical MW |
28.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Control |
|
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles. |
| Buffer |
PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative |
0.02% Sodium Azide |
| Concentration |
1 mg/ml |
| Purity |
Immunogen affinity purified |
Alternate Names for MYF-5 Antibody (OTI2G5)
Background
nvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for MYF-5 Antibody (NBP2-44245) (0)
![Western Blot: MYF-5 Antibody (OTI2G5) [NBP2-44245] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYF5 , (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYF5](http://images.novusbio.com/fullsize/MYF-5-Antibody-OTI2G5-Western-Blot-NBP2-44245-img0001.jpg "Western Blot: MYF-5 Antibody (OTI2G5) [NBP2-44245] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYF5 , (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYF5")
![Immunohistochemistry-Paraffin: MYF-5 Antibody (OTI2G5) [NBP2-44245] - Carcinoma of Human prostate tissue using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MYF-5-Antibody-OTI2G5-Immunohistochemistry-Paraffin-NBP2-44245-img0012.jpg "Immunohistochemistry-Paraffin: MYF-5 Antibody (OTI2G5) [NBP2-44245] - Carcinoma of Human prostate tissue using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry-Paraffin: MYF-5 Antibody (OTI2G5) [NBP2-44245] - Human breast tissue within the normal limits using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MYF-5-Antibody-OTI2G5-Immunohistochemistry-Paraffin-NBP2-44245-img0004.jpg "Immunohistochemistry-Paraffin: MYF-5 Antibody (OTI2G5) [NBP2-44245] - Human breast tissue within the normal limits using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry-Paraffin: MYF-5 Antibody (OTI2G5) [NBP2-44245] - Carcinoma of Human kidney tissue using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MYF-5-Antibody-OTI2G5-Immunohistochemistry-Paraffin-NBP2-44245-img0005.jpg "Immunohistochemistry-Paraffin: MYF-5 Antibody (OTI2G5) [NBP2-44245] - Carcinoma of Human kidney tissue using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry-Paraffin: MYF-5 Antibody (OTI2G5) [NBP2-44245] - Human Ovary tissue within the normal limits using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MYF-5-Antibody-OTI2G5-Immunohistochemistry-Paraffin-NBP2-44245-img0007.jpg "Immunohistochemistry-Paraffin: MYF-5 Antibody (OTI2G5) [NBP2-44245] - Human Ovary tissue within the normal limits using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry-Paraffin: MYF-5 Antibody (OTI2G5) [NBP2-44245] - Human pancreas tissue within the normal limits using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MYF-5-Antibody-OTI2G5-Immunohistochemistry-Paraffin-NBP2-44245-img0008.jpg "Immunohistochemistry-Paraffin: MYF-5 Antibody (OTI2G5) [NBP2-44245] - Human pancreas tissue within the normal limits using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry-Paraffin: MYF-5 Antibody (OTI2G5) [NBP2-44245] - Carcinoma of Human thyroid tissue using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MYF-5-Antibody-OTI2G5-Immunohistochemistry-Paraffin-NBP2-44245-img0010.jpg "Immunohistochemistry-Paraffin: MYF-5 Antibody (OTI2G5) [NBP2-44245] - Carcinoma of Human thyroid tissue using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")