Hu,
Mu,
Rt,
Po,
Bt,
Bv,
ChHa,
Ma,
Ma-Mn,
PmSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bt, Bv, ChHa, Ma, Ma-Mn, Pm
WB, Flow, ICC/IF
Applications: WB, Flow, ICC/IF
Host: Rabbit Polyclonal
Alt Catalog# IMG-514
Sample Size Available
Sizes: Sample Size Available
![Western Blot: Lamin A + C Antibody (4C4) [NBP2-25151] - Analysis of different cell lysates using mouse mAb to lamin A/C, NBP2-25151, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293 [4] C6, and [5] NIH-3T3 cell lysates. Two strong bands at 74 and 65kDa correspond to the lamin A and lamin C proteins respectively, detected only in the cells of human origin. NBP2-25151 antibody failed to recognize rat or mouse proteins.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4C4-Western-Blot-NBP2-25151-img0006.jpg "Western Blot: Lamin A + C Antibody (4C4) [NBP2-25151] - Analysis of different cell lysates using mouse mAb to lamin A/C, NBP2-25151, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293 [4] C6, and [5] NIH-3T3 cell lysates. Two strong bands at 74 and 65kDa correspond to the lamin A and lamin C proteins respectively, detected only in the cells of human origin. NBP2-25151 antibody failed to recognize rat or mouse proteins.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151] - Analysis of HeLa cells stained with mouse mAb to lamin A/C, NBP2-25151, dilution 1:2,000 in red, and costained with rabbit pAb to HSP60, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. NBP2-25151 antibody specifically labels the nuclear lamina, while the HSP60 antibody reveals protein expressed in mitochondria.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4C4-Immunocytochemistry-Immunofluorescence-NBP2-25151-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4C4) [NBP2-25151] - HeLa cells staing with NBP2-25151 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25151 reveals strong nuclear lamina staining, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4C4-Immunocytochemistry-Immunofluorescence-NBP2-25151-img0004.jpg)
![Western Blot: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of various amounts of Human fibroblast whole cell lysate.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Western-Blot-NB100-74451-img0017.jpg "Western Blot: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of various amounts of Human fibroblast whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C (green) in Human fibroblast cells. Methanol fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C, at a dilution of 1:200 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0016.jpg)
![Western Blot: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of 10ug nuclear extract from fractionated mouse tissue lysate.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Western-Blot-NB100-74451-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C (red) in HeLa cells at high magnification.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C using Lamin A/C Monoclonal Antibody (mab636) shows staining in Hela Cells. Lamin A/C (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Lamin A/C at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C using Lamin A/C Monoclonal Antibody (mab636) shows staining in U251 Cells. Lamin A/C (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Lamin A/C at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C in HMVEC Cells.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C in A549 Cells.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C (green) in untreated U2-OS cells (A) or HeLa cells (B). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 0.3% BSA for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C, at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0015.jpg)
![Knockout Validated: Lamin A + C Antibody [NBP2-19324] - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody. The HRP-conjugated anti-rabbit IgG (NBP2-19301) antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-19324-img0017.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Lamin A + C Antibody [NBP2-19324] - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody. The HRP-conjugated anti-rabbit IgG (NBP2-19301) antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-19324] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Lamin A + C stained by Lamin A + C antibody diluted at 1:1000. Red: alpha Tubulin, stained by alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19324-img0020.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Mouse stomach. Lamin A + C stained by Lamin A + C antibody diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0022.jpg)
![Western Blot: Lamin A + C Antibody [NBP2-19324] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-19324-img0009.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - C2C12 xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - RT2 xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0005.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Human breast carcinoma. Lamin A + C antibody diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Mahlarvu xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0018.jpg)
![Immunoprecipitation: Lamin A + C Antibody [NBP2-19324] - A. 50 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of Lamin A + C protein by 4 ug Lamin A + C antibody (NBP2-19324)7.5 % SDS-PAGE](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunoprecipitation-NBP2-19324-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Lamin A + C Antibody [NBP2-19324] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-19324_rabbit-polyclonal-lamin-a-c-antibody-1610202419414527.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Lamin A + C Antibody [NBP2-19324] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-19324_rabbit-polyclonal-lamin-a-c-antibody-1610202419405760.jpg)
![Western Blot: Lamin A + C Antibody [NB100-56649] - Analysis of human Lamin A + C in 15 ug of HeLa cell lysate using NB100-56649 at 1:1000.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NB100-56649-img0003.jpg "Western Blot: Lamin A + C Antibody [NB100-56649] - Analysis of human Lamin A + C in 15 ug of HeLa cell lysate using NB100-56649 at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NB100-56649] - Lamin A/C antibody was tested in A431 cells at 1:100 against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56649-img0005.jpg)
![Flow (Intracellular): Lamin A + C Antibody [NB100-56649] - Intracellular staining of HeLa cells (1 x 10^6 cells/mL) with Lamin A+C antibody (orange) stained at 1:1000. Detected with a GtxRb DyLight 488 secondary. Shown with the secondary only control (blue).](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Flow-Intracellular-NB100-56649-img0004.jpg)
![Western Blot: Lamin A + C Antibody [NBP2-25153] - Stripe blot of crude HeLa cell extract. Note two strong and clean bands at 74kDa and 65kDa, corresponding to Lamin A and C.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-25153-img0001.jpg "Western Blot: Lamin A + C Antibody [NBP2-25153] - Stripe blot of crude HeLa cell extract. Note two strong and clean bands at 74kDa and 65kDa, corresponding to Lamin A and C.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-25153] - HeLa cells stained with NBP2-25153 (red) counterstained with monoclonal antibody to Hsp27, NBP2-25149 (green) and DNA (blue). NBP2-25153 reveals strong nuclear lamina staining, while NBP2-25149 reveals strong cytoplasmic staining. Since both DNA (blue) and Lamin A + C (red) are associated with the nuclear compartment, this region appears crimson in this image.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25153-img0003.jpg)
![Western Blot: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Western blot analysis of extracts of various cell lines, using Lamin A + C antibody (NBP3-15754) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Western-Blot-NBP3-15754-img0010.jpg "Western Blot: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Western blot analysis of extracts of various cell lines, using Lamin A + C antibody (NBP3-15754) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunofluorescence analysis of U2OS cells using Lamin A + C antibody (NBP3-15754) at dilution of 1:100 - 1:1600. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Immunocytochemistry-Immunofluorescence-NBP3-15754-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Lamin A + C antibody (NBP3-15754) at dilution of 1:400 - 1:3200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Immunohistochemistry-Paraffin-NBP3-15754-img0003.jpg)
![Western Blot: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Western blot analysis of extracts of HeLa cells, using Lamin A + C antibody (NBP3-15754) at 1:10000-1:2560000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Western-Blot-NBP3-15754-img0008.jpg)
![Western Blot: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Western blot analysis of extracts of various cell lines, using Lamin A + C antibody (NBP3-15754) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Western-Blot-NBP3-15754-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunofluorescence analysis of H9C2 cells using Lamin A + C antibody (NBP3-15754) at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Immunocytochemistry-Immunofluorescence-NBP3-15754-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunofluorescence analysis of L929 cells using Lamin A + C antibody (NBP3-15754) at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Immunocytochemistry-Immunofluorescence-NBP3-15754-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Confocal immunofluorescence analysis of HeLa cells using Lamin A + C antibody (NBP3-15754) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Immunocytochemistry-Immunofluorescence-NBP3-15754-img0006.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunohistochemistry of paraffin-embedded Human breast cancer using Lamin A + C antibody (NBP3-15754) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Immunohistochemistry-Paraffin-NBP3-15754-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Western blot analysis of extracts from normal (control) and Lamin A + C knockout (KO) HeLa cells, using Lamin A + C antibody (NBP3-15754) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Knockout-Validated-NBP3-15754-img0001.jpg)
![Immunoprecipitation: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunoprecipitation analysis of 200ug extracts of K-562 cells using 3ug Lamin A + C antibody (NBP3-15754). Western blot was performed from the immunoprecipitate using Lamin A + C antibody (NBP3-15754) at a dilition of 1:20000.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-4L8Q0-Immunoprecipitation-NBP3-15754-img0011.jpg)
![Western Blot: Lamin A + C Antibody [NBP1-85550] - Lamin A Antibody [NBP1-85550] - Analysis in human cell lines PC-3 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/Lamin-A-Antibody-Western-Blot-NBP1-85550-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Lamin A + C Antibody [NBP1-85550] - Lamin A Antibody [NBP1-85550] - Analysis in human cell lines PC-3 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Lamin A Antibody [NBP1-85550] - Staining of human fallopian tube shows moderate positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/Lamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-85550-img0013.jpg)
![Immunohistochemistry-Paraffin: Lamin A Antibody [NBP1-85550] - Staining of human prostate shows strong positivity in nuclear membrane in smooth muscle cells and glandular cells.](http://images.novusbio.com/fullsize/Lamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-85550-img0011.jpg)
![Immunohistochemistry-Paraffin: Lamin A Antibody [NBP1-85550] - Staining of human stomach shows strong positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/Lamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-85550-img0012.jpg)
![Immunohistochemistry-Paraffin: Lamin A Antibody [NBP1-85550] - Staining of human skin shows strong positivity in nuclear membrane in squamous epithelial cells.](http://images.novusbio.com/fullsize/Lamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-85550-img0014.jpg)
![Western Blot: Rabbit Polyclonal Lamin A + C Antibody [NBP1-85550] - Western blot showing over-expression of lamin A/C tagged with mKate, as well as endogenous lamin A/C, in zebrafish embryos. Actin used as loading control. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-85550_rabbit-polyclonal-lamin-a-c-antibody-western-blot-722024124819..jpg)
![Knockout Validated: Lamin A + C Antibody [NBP2-19323] - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-19323-img0004.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Lamin A + C Antibody [NBP2-19323] - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Western Blot: Lamin A + C Antibody [NBP2-19323] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-19323-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-19323] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using Lamin A/C antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19323-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded human breast carcinoma. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0012.jpg)
![Western Blot: Lamin A + C Antibody [NBP2-19323] - A. 30 ug C2C12 whole cell lysate/extract 7.5 % SDS-PAGE Lamin A + C antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-19323-img0005.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Lamin A/C antibody detects LMNA protein at membrane on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Lamin A/C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0002.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse prostate. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse fore brain. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0008.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded mouse skin. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0009.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19323] - Paraffin-embedded human endometrial carcinoma. Lamin A + C antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19323-img0011.jpg)
![Immunoprecipitation: Lamin A + C Antibody [NBP2-19323] - Immunoprecipitation of Lamin A + C protein from HeLa whole cell extracts using 5 ug of Lamin A + C antibody. Western blot analysis was performed using Lamin A + C antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunoprecipitation-NBP2-19323-img0010.jpg)
![Western Blot: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunoblotting result of NBP1-97687(131C3) recognizing nuclear lamins A and C in human fibroblasts.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-131C3-Western-Blot-NBP1-97687-img0003.jpg "Western Blot: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunoblotting result of NBP1-97687(131C3) recognizing nuclear lamins A and C in human fibroblasts.")
![Immunohistochemistry-Frozen: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry on frozen tissue section of human skin showing nuclear lamina staining in epithelial and connective tissue cells.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-131C3-Immunohistochemistry-Frozen-NBP1-97687-img0004.jpg)
![Immunohistochemistry: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry of on frozen sections of swine skin showing strong positive staining in nuclei of the epidermal cells and to a lesser extent in the connective tissue.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-131C3-Immunohistochemistry-NBP1-97687-img0001.jpg)
![Immunohistochemistry-Frozen: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry of NBP1-97687(131C3) on frozen sections of human colon showing strong positive staining in nuclei of the epithelial cells and to a lesser extent in the connective tissue.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-131C3-Immunohistochemistry-Frozen-NBP1-97687-img0002.jpg)
![Western Blot: Lamin A + C Antibody [NBP2-25152] - Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken pAb to lamin A/C, NBP2-25152, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 74 and 65kDa correspond to lamin A and lamin C proteins respectively, detected exclusively in the nuclear fractions. The same blot was simultaneously probed with mouse mAb to GAPDH, in red. The single band at 37kDa represents GAPDH protein which is expressed predominantly in the cytosolic fractions.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NBP2-25152-img0002.jpg "Western Blot: Lamin A + C Antibody [NBP2-25152] - Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken pAb to lamin A/C, NBP2-25152, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 74 and 65kDa correspond to lamin A and lamin C proteins respectively, detected exclusively in the nuclear fractions. The same blot was simultaneously probed with mouse mAb to GAPDH, in red. The single band at 37kDa represents GAPDH protein which is expressed predominantly in the cytosolic fractions.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-25152] - Analysis of HeLa cells stained with chicken pAb to lamin A/C, NBP2-25152, dilution 1:2,000 in red, and costained with mouse mAb to actin, dilution 1:500, in green. The blue is Hoechst staining of nuclear DNA. The CPCA-LaminAC antibody specifically labels the nuclear lamina, while the actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunofluorescence-NBP2-25152-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (133A2) [NBP1-97688] - Staining of fiboblasts showing nuclear lamina](http://images.novusbio.com/fullsize/Lamin-A-Antibody-133A2-Immunocytochemistry-Immunofluorescence-NBP1-97688-img0001.jpg "Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (133A2) [NBP1-97688] - Staining of fiboblasts showing nuclear lamina")
![Immunohistochemistry-Paraffin: Lamin A + C Antibody (133A2) [NBP1-97688] - Analysis of human colon.](http://images.novusbio.com/fullsize/Lamin-A-Antibody-133A2-Immunohistochemistry-Paraffin-NBP1-97688-img0004.jpg)
![Immunohistochemistry-Frozen: Lamin A + C Antibody (133A2) [NBP1-97688] - Frozen sections of human colon showing nuclear lamina staining in epithelial and connective tissue cells](http://images.novusbio.com/fullsize/Lamin-A-Antibody-133A2-Immunohistochemistry-Frozen-NBP1-97688-img0005.jpg)
![Western Blot: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Lamin A + C (WL4G10) [NBP2-59933] - Whole cell lysates from HeLa and 293T cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and stained with WL4G10 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. As expected, 293T cells express less of the lamin A isoform.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Western-Blot-NBP2-59933-img0006.jpg "Western Blot: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Lamin A + C (WL4G10) [NBP2-59933] - Whole cell lysates from HeLa and 293T cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and stained with WL4G10 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. As expected, 293T cells express less of the lamin A isoform.")
![Immunohistochemistry-Paraffin: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Analysis of formalin-fixed paraffin-embedded human gut. Following heat induced antigen retrieval, the sections were stained with mouse monoclonal anti-lamin A+C clone WL4G10 antibody (green). Nuclei were visualized with DAPI.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Confocal images of methanol fixed Hela cells that were immunofluorescently stained with mouse monoclonal WL4G10 antibody. The antibody works as well on cells fixed with 4% PFA.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (WL4G10) [NBP2-59933] - The mouse monoclonal WL4G10 antibody recognizes human lamins A and C. Immortalized mouse adult LMNA null fibroblast stably expressing: (A) mEos2-lamin A or (B) mEos2- lamin C fused were fixed in 4% PFA and stained with WL4G10 antibody (magenta). Expression of the respective LMNA isoform is shown in green. The nuclei were counter-stained with Hoechst. Of note, the WL4G10 antibody does not recognize mouse lamins A and C.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0003.jpg)