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![Immunocytochemistry/Immunofluorescence: Lyp/PTPN22 Antibody [NBP2-69989] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69989-img0001.jpg "Immunocytochemistry/Immunofluorescence: Lyp/PTPN22 Antibody [NBP2-69989] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989] - (3.75ug/ml) Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Immunohistochemistry-Paraffin-NBP2-69989-img0005.jpg)
![Flow Cytometry: Lyp/PTPN22 Antibody [NBP2-69989] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Flow-Cytometry-NBP2-69989-img0002.jpg)
![Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989] - (3.75ug/ml) Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Immunohistochemistry-Paraffin-NBP2-69989-img0003.jpg)
![Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989] - (3.75ug/ml) Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Immunohistochemistry-Paraffin-NBP2-69989-img0004.jpg)
![Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01] - Analysis of PTPN22 expression in transfected 293T cell line by PTPN22 monoclonal antibody (M01), clone 4F6.Lane 1: PTPN22 transfected lysate(85.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-4F6-Western-Blot-H00026191-M01-img0006.jpg "Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01] - Analysis of PTPN22 expression in transfected 293T cell line by PTPN22 monoclonal antibody (M01), clone 4F6.Lane 1: PTPN22 transfected lysate(85.1 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01] - Analysis of PTPN22 over-expressed 293 cell line, cotransfected with PTPN22 Validated Chimera RNAi ( Cat # H00026191-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PTPN22 monoclonal antibody (M01), clone 4F6 (Cat # H00026191-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-4F6-Western-Blot-H00026191-M01-img0004.jpg)
![ELISA: Lyp/PTPN22 Antibody (4F6) [H00026191-M01] - Detection limit for recombinant GST tagged PTPN22 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-4F6-ELISA-H00026191-M01-img0007.jpg)
![SDS-Page: Recombinant Human Lyp/PTPN22 Protein [H00026191-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Lyp-PTPN22-Protein-SDS-Page-H00026191-P01-img0002.jpg "SDS-Page: Recombinant Human Lyp/PTPN22 Protein [H00026191-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human Lyp/PTPN22 Protein [H00026191-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00026191-Q01-1.jpg "SDS-Page: Recombinant Human Lyp/PTPN22 Protein [H00026191-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: Lyp/PTPN22 Antibody (1A6) [H00026191-M02] - Analysis of monoclonal antibody to PTPN22 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-1A6-Immunocytochemistry-Immunofluorescence-H00026191-M02-img0002.jpg "Immunocytochemistry/Immunofluorescence: Lyp/PTPN22 Antibody (1A6) [H00026191-M02] - Analysis of monoclonal antibody to PTPN22 on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP1-81277] - Staining of human lymph node shows stark cytoplasmic positivity in a subset of germinal center cells and non-germinal center cells.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Immunohistochemistry-Paraffin-NBP1-81277-img0005.jpg "Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP1-81277] - Staining of human lymph node shows stark cytoplasmic positivity in a subset of germinal center cells and non-germinal center cells.")
![Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP1-81276] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Immunohistochemistry-Paraffin-NBP1-81276-img0005.jpg "Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP1-81276] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.")
![Western Blot: PTPN22 Overexpression Lysate (Adult Normal) [NBL1-14973] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PTPN22.](http://images.novusbio.com/fullsize/PTPN22-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14973-img0002.jpg "Western Blot: PTPN22 Overexpression Lysate (Adult Normal) [NBL1-14973] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PTPN22.")