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![Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Analysis in human cell lines A-431 and HEK293 using anti-PTK2B antibody. Corresponding PTK2B RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Western-Blot-NBP1-91228-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Analysis in human cell lines A-431 and HEK293 using anti-PTK2B antibody. Corresponding PTK2B RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Analysis using Anti-PTK2B antibody NBP1-91228 (A) shows similar pattern to independent antibody NBP1-91227 (B).](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Western-Blot-NBP1-91228-img0014.jpg)
![Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody [NBP1-91228] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Immunohistochemistry-Paraffin-NBP1-91228-img0009.jpg)
![Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Human breast cancer cell line MDA-MB-231 was treated with carboplatin for 72 hours and the expression of phospho-PYK2 and total PYK2 were detected by Western blot. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Western-Blot-NBP1-91228-img0013.jpg)
![Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Western-Blot-NBP1-91228-img0012.jpg)
![Western Blot: PYK2/FAK2 Antibody [NBP3-12969] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 antibody (NBP3-12969) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Western-Blot-NBP3-12969-img0005.jpg "Western Blot: PYK2/FAK2 Antibody [NBP3-12969] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 antibody (NBP3-12969) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody [NBP3-12969] - PYK2/FAK2 antibody detects PYK2/FAK2 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PYK2/FAK2 stained by PYK2/FAK2 antibody (NBP3-12969) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12969-img0006.jpg)
![Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody [NBP3-12969] - PYK2/FAK2 antibody detects PYK2/FAK2 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. PYK2/FAK2 antibody (NBP3-12969) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Immunohistochemistry-Paraffin-NBP3-12969-img0004.jpg)
![Western Blot: PYK2/FAK2 Antibody [NBP3-12969] - PYK2/FAK2 antibody detects PYK2/FAK2 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 antibody (NBP3-12969) diluted at a dilution of 1:1000.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Western-Blot-NBP3-12969-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody [NBP3-12969] - PYK2/FAK2 antibody detects PYK2/FAK2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PYK2/FAK2 protein stained by PYK2/FAK2 antibody (NBP3-12969) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12969-img0002.jpg)
![Immunoprecipitation: PYK2/FAK2 Antibody [NBP3-12969] - Immunoprecipitation of PYK2/FAK2 protein from A431 whole cell extracts using 5 ug of PYK2/FAK2 antibody (NBP3-12969). Western blot analysis was performed using PYK2/FAK2 antibody (NBP3-12969) diluted at 1:500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Immunoprecipitation-NBP3-12969-img0003.jpg)
![Western Blot: PYK2/FAK2 Antibody (GT858) [NBP3-13592] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 antibody [GT858] (NBP3-13592) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-GT858-Western-Blot-NBP3-13592-img0001.jpg "Western Blot: PYK2/FAK2 Antibody (GT858) [NBP3-13592] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 antibody [GT858] (NBP3-13592) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (GT858) [NBP3-13592] - PYK2/FAK2 antibody [GT858] detects PYK2/FAK2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. PYK2/FAK2 stained by PYK2/FAK2 antibody [GT858] (NBP3-13592) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-GT858-Immunohistochemistry-Paraffin-NBP3-13592-img0004.jpg)
![Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (GT858) [NBP3-13592] - PYK2/FAK2 antibody [GT858] detects PYK2/FAK2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PYK2/FAK2 stained by PYK2/FAK2 antibody [GT858] (NBP3-13592) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-GT858-Immunohistochemistry-Paraffin-NBP3-13592-img0002.jpg)
![Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (GT858) [NBP3-13592] - PYK2/FAK2 antibody [GT858] detects PYK2/FAK2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat thymus gland. PYK2/FAK2 stained by PYK2/FAK2 antibody [GT858] (NBP3-13592) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-GT858-Immunohistochemistry-Paraffin-NBP3-13592-img0003.jpg)
![Western Blot: PYK2/FAK2 Antibody (1F9) [H00002185-M01] - Analysis of PTK2B expression in transfected 293T cell line by PTK2B monoclonal antibody (M01), clone 1F9.Lane 1: PTK2B transfected lysate(115.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-1F9-Western-Blot-H00002185-M01-img0008.jpg "Western Blot: PYK2/FAK2 Antibody (1F9) [H00002185-M01] - Analysis of PTK2B expression in transfected 293T cell line by PTK2B monoclonal antibody (M01), clone 1F9.Lane 1: PTK2B transfected lysate(115.9 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: PYK2/FAK2 Antibody (1F9) [H00002185-M01] - PTK2B monoclonal antibody (M01), clone 1F9 Analysis of PTK2B expression in K-562.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-1F9-Western-Blot-H00002185-M01-img0007.jpg)
![Immunoprecipitation: PYK2/FAK2 Antibody (1F9) [H00002185-M01] - Analysis of PTK2B transfected lysate using anti-PTK2B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PTK2B MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-1F9-Immunoprecipitation-H00002185-M01-img0005.jpg)
![ELISA: PYK2/FAK2 Antibody (1F9) [H00002185-M01] - Detection limit for recombinant GST tagged PTK2B is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-1F9-ELISA-H00002185-M01-img0009.jpg)
![Western Blot: PYK2/FAK2 [p Tyr402] Antibody [NBP3-13314] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 (phospho Tyr402) antibody (NBP3-13314) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PYK2-FAK2-[p-Tyr402]-Antibody-Western-Blot-NBP3-13314-img0003.jpg "Western Blot: PYK2/FAK2 [p Tyr402] Antibody [NBP3-13314] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 (phospho Tyr402) antibody (NBP3-13314) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: PYK2/FAK2 [p Tyr402] Antibody [NBP3-13314] - Untreated (-) and treated (+) HCT116 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 (phospho Tyr402) antibody (NBP3-13314) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PYK2-FAK2-[p-Tyr402]-Antibody-Western-Blot-NBP3-13314-img0001.jpg)
![Western Blot: PYK2/FAK2 [p Tyr402] Antibody [NBP3-13314] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 (phospho Tyr402) antibody (NBP3-13314) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PYK2-FAK2-[p-Tyr402]-Antibody-Western-Blot-NBP3-13314-img0002.jpg)
![Western Blot: PYK2/FAK2 Antibody (GT1132) [NBP3-13573] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 antibody [GT1132] (NBP3-13573) diluted at 1:5000.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-GT1132-Western-Blot-NBP3-13573-img0001.jpg "Western Blot: PYK2/FAK2 Antibody (GT1132) [NBP3-13573] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 antibody [GT1132] (NBP3-13573) diluted at 1:5000.")
![Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (GT1132) [NBP3-13573] - PYK2/FAK2 antibody [GT1132] detects PYK2/FAK2 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PYK2/FAK2 antibody [GT1132] (NBP3-13573) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-GT1132-Immunohistochemistry-Paraffin-NBP3-13573-img0002.jpg)
![Western Blot: PYK2/FAK2 Antibody [NBP1-91227] - Analysis using Anti-PTK2B antibody NBP1-91227 (A) shows similar pattern to independent antibody NBP1-91228 (B).](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Western-Blot-NBP1-91227-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PYK2/FAK2 Antibody [NBP1-91227] - Analysis using Anti-PTK2B antibody NBP1-91227 (A) shows similar pattern to independent antibody NBP1-91228 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody [NBP1-91227] - Staining of human cerebral cortex shows moderate positivity in neuronal and glial cells and in neuropil.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Immunohistochemistry-Paraffin-NBP1-91227-img0006.jpg)
![Western Blot: PYK2/FAK2 Antibody (M229) [NBP3-23221] - Western blot analysis of mouse brain. The blot was probed with anti-PYK2/FAK2 (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3), and 1:2000 (lane 4).](http://images.novusbio.com/fullsize/nbp3-23221_mouse-pyk2-fak2-mab-m229-199202312403720.jpg "Western Blot: PYK2/FAK2 Antibody (M229) [NBP3-23221] - Western blot analysis of mouse brain. The blot was probed with anti-PYK2/FAK2 (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3), and 1:2000 (lane 4).")
![Western Blot: PYK2/FAK2 [p Tyr402] Antibody (M269) [NBP3-23222] - Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-PYK2/FAK2 (C-terminal region) antibody (lanes 1 & 2) or anti-PYK2/FAK2 (Tyr-402) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23222_mouse-pyk2-fak2-p-tyr402-mab-m269-19920231992817.jpg "Western Blot: PYK2/FAK2 [p Tyr402] Antibody (M269) [NBP3-23222] - Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-PYK2/FAK2 (C-terminal region) antibody (lanes 1 & 2) or anti-PYK2/FAK2 (Tyr-402) (lanes 3 & 4).")