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PYK2/FAK2 Antibody (M229)

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Western Blot: PYK2/FAK2 Antibody (M229) [NBP3-23221] - Western blot analysis of mouse brain. The blot was probed with anti-PYK2/FAK2 (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3), and 1:2000 (lane 4).
Western Blot: PYK2/FAK2 Antibody (M229) [NBP3-23221] - Western blot analysis of mouse brain. The blot was probed with anti-PYK2/FAK2 (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3), and ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
M229
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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PYK2/FAK2 Antibody (M229) Summary

Description
Stable for at least 1 year at -20°C.
Immunogen
Clone M229 was generated from a recombinant human PYK2 protein that included amino acids residues in the C-terminal region. This sequence is conserved in rat and mouse PYK2/FAK2, and has low homology to other related kinases. [UniProt# Q14289]
Specificity
The antibody detects a 116 kDa band corresponding to PYK2/FAK2 on SDS-PAGE immunoblots of mouse brain tissue.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PTK2B
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
Theoretical MW
116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C.
Buffer
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for PYK2/FAK2 Antibody (M229)

  • CADTK
  • CADTKCAK-beta
  • CAK beta
  • CAKB
  • Calcium-dependent tyrosine kinase
  • Cell adhesion kinase beta
  • EC 2.7.10
  • FADK2
  • FAK2
  • FAK2EC 2.7.10.2
  • Focal adhesion kinase 2
  • FRNK
  • PKB
  • ppyk2
  • Proline-rich tyrosine kinase 2
  • protein kinase B
  • protein tyrosine kinase 2 beta
  • protein-tyrosine kinase 2-beta
  • PTK
  • PTK2B protein tyrosine kinase 2 beta
  • PTK2B
  • PYK2
  • PYK2Related adhesion focal tyrosine kinase
  • RAFTK
  • RAFTKFADK 2

Background

Proline rich protein tyrosine kinase (Pyk2) is a focal adhesion kinase (FAK2) related protein kinase protein also known as cell adhesion kinase (CAK ), related adhesion focal tyrosine kinase (RAFTK), or calcium-dependent PTK (CADTK) (3.4). Highly expre ed in the nervous system, protein tyrosine kinase (Pyk2) is phosphorylated on the tyrosine residue by a variety of G-protein-coupled receptors & extracellular signals that increase the level of Ca+ intracellulary (1,2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PTK2B