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PYK2/FAK2 [p Tyr402] Antibody (M269)

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Western Blot: PYK2/FAK2 [p Tyr402] Antibody (M269) [NBP3-23222] - Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-PYK2/FAK2 (C-terminal region) antibody (lanes 1 & 2) or anti-PYK2/FAK2 (Tyr-402) (lanes 3 & 4).
Western Blot: PYK2/FAK2 [p Tyr402] Antibody (M269) [NBP3-23222] - Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
M269
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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PYK2/FAK2 [p Tyr402] Antibody (M269) Summary

Description
Stable for at least 1 year at -20°C.
Immunogen
Clone M269 was generated from a phosphopeptide containing amino acids surrounding tyrosine 402 in human PYK2/FAK2. This sequence is conserved in rat and mouse PYK2/FAK2, and has significant homology to the conserved site in FAK. [UniProt# Q14289]
Modification
p Tyr402
Specificity
The antibody detects a 116 kDa band corresponding to PYK2/FAK2 on SDS-PAGE immunoblots of human A431, Jurkat and K562 cells treated with pervanadate, but is not observed in control, untreated cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PTK2B
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Theoretical MW
116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C.
Buffer
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for PYK2/FAK2 [p Tyr402] Antibody (M269)

  • CADTK
  • CADTKCAK-beta
  • CAK beta
  • CAKB
  • Calcium-dependent tyrosine kinase
  • Cell adhesion kinase beta
  • EC 2.7.10
  • FADK2
  • FAK2
  • FAK2EC 2.7.10.2
  • Focal adhesion kinase 2
  • FRNK
  • PKB
  • ppyk2
  • Proline-rich tyrosine kinase 2
  • protein kinase B
  • protein tyrosine kinase 2 beta
  • protein-tyrosine kinase 2-beta
  • PTK
  • PTK2B protein tyrosine kinase 2 beta
  • PTK2B
  • PYK2
  • PYK2Related adhesion focal tyrosine kinase
  • RAFTK
  • RAFTKFADK 2

Background

Proline rich protein tyrosine kinase (Pyk2) is a focal adhesion kinase (FAK2) related protein kinase protein also known as cell adhesion kinase (CAK ), related adhesion focal tyrosine kinase (RAFTK), or calcium-dependent PTK (CADTK) (3.4). Highly expre ed in the nervous system, protein tyrosine kinase (Pyk2) is phosphorylated on the tyrosine residue by a variety of G-protein-coupled receptors & extracellular signals that increase the level of Ca+ intracellulary (1,2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PTK2B