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![Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - The decrease in protein expressions of c-kit, Choline Acetyltransferase/ChAT, nNOS and Cx43 in the colon of elderly humans. Expressions of c-kit, ChAT, nNOS and Cx43 proteins in colonic muscle layers detached from youth and older adults were examined by western blotting (n=4 per group), and similar reduction was observed. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30530917/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Choline Acetyltransferase-ChAT Antibody-Western Blot-NBP1-30052-img0006.jpg "Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - The decrease in protein expressions of c-kit, Choline Acetyltransferase/ChAT, nNOS and Cx43 in the colon of elderly humans. Expressions of c-kit, ChAT, nNOS and Cx43 proteins in colonic muscle layers detached from youth and older adults were examined by western blotting (n=4 per group), and similar reduction was observed. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30530917/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Representative images of lumbar spinal cord sections at 130 days immunostained with Choline Acetyltransferase/ChAT. Scale bar, 200 um. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201808888), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30052-img0005.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brainstem showing specific labeling using the anti-ChAT antibody. Image courtesy of Dr. Robert Sloviter, University of Arizona.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Paraffin-NBP1-30052-img0003.jpg)
![Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brain lysate showing specific immunolabeling of the ~70k ChAT.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Western-Blot-NBP1-30052-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Immunostaining of anterior horn of lumbar spinal cord for Choline Acetyltransferase/ChAT (in green) and p62 (in red) at 130 days. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201808888), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30052-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - MATR3 loss in alpha -motor neurons & interneurons in the thoracic spinal cord of Matr3S85C/S85C mice. (A) Representative images of 10 & 30 weeks thoracic spinal cord staining for alpha -motor neurons (ChAT+, NeuN+, denoted by white arrows) & gamma -motor neurons (Chat+, NeuN−, denoted by yellow arrows). Scale bar for the spinal cord image denotes 500 μm & the zoomed-in image denotes 50 µm. Quantification of the percentage of motor neurons with reduced MATR3 staining in (B) alpha -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C) & (C) gamma -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). Quantification of the number of (D) alpha -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C) & (E) gamma -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). (F) Representative images of 10 & 30 weeks PVALB+ interneurons in the thoracic spinal cord. Interneurons with reduced MATR3 staining are denoted by a white asterisk. Scale bar denotes 50 µm. (G) Quantification of the percentage of PVALB+ interneurons with reduced MATR3 staining (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). (H) Quantification of the number of PVALB+ interneurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). Bar graph heights depict mean ± SEM, with each datapoint representing an animal. * p < 0.05, *** p < 0.001, **** p < 0.0001, ns = not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35205163), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30052_goat-polyclonal-choline-acetyltransferase-chat-antibody-310202416173537.jpg)
![Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - MATR3 loss in alpha -motor neurons & interneurons in the cervical spinal cord of Matr3S85C/S85C mice. (A) Representative images of 10 & 30 weeks cervical spinal cord staining for alpha -motor neurons (ChAT+, NeuN+, denoted by white arrows) & gamma -motor neurons (ChAT+, NeuN−, denoted by yellow arrows). Scale bar for the spinal cord image denotes 500 μm & the zoomed-in image denotes 50 µm. Quantification of the percentage of motor neurons with reduced MATR3 staining in (B) alpha -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C) & (C) gamma -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). Quantification of the number of (D) alpha -motor neurons (10 weeks: n = 6 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C) & (E) gamma -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). (F) Representative images of 10 & 30 weeks PVALB+ interneurons in the cervical spinal cord. Interneurons with reduced MATR3 staining are denoted by a white asterisk. Scale bar denotes 50 µm. (G) Quantification of the percentage of PVALB+ interneurons with reduced MATR3 staining (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). (H) Quantification of the number of PVALB+ interneurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). Bar graph heights depict mean ± SEM, with each datapoint representing an animal. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35205163), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30052_goat-polyclonal-choline-acetyltransferase-chat-antibody-310202416165640.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining in human placenta and liver tissues. Corresponding CHAT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3173-Immunohistochemistry-Paraffin-NBP2-46620-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining in human placenta and liver tissues. Corresponding CHAT RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining of SK-MEL-30 cells using the Anti-CHAT monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3173-Immunocytochemistry-Immunofluorescence-NBP2-46620-img0021.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3173-Immunohistochemistry-Paraffin-NBP2-46620-img0022.jpg)
![Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining of mouse brain shows strong positivity in acetylcholine neurons in the basal forebrain.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3173-Immunohistochemistry-NBP2-46620-img0010.jpg)
![Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining of mouse basal forebrain shows strong positivity in acetylcholine neurons in the caudate putamen.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3173-Immunohistochemistry-NBP2-46620-img0011.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining of human cerebral cortex shows moderate positivity in cholinergic neural fibers.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3173-Immunohistochemistry-Paraffin-NBP2-46620-img0012.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining of human placenta shows strong cytoplasmic positivity in a subset of cells in chorionic villi.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3173-Immunohistochemistry-Paraffin-NBP2-46620-img0013.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3173-Immunohistochemistry-Paraffin-NBP2-46620-img0014.jpg)
![Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining of rat brain shows strong positivity in acetylcholine neurons in the basal forebrain.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3173-Immunohistochemistry-NBP2-46620-img0016.jpg)
![Western Blot: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - Effect of denaturing temperature on Western blot on brain lysates using Rabbit antibody to ChAT.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Western-Blot-NB110-89724-img0018.jpg "Western Blot: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - Effect of denaturing temperature on Western blot on brain lysates using Rabbit antibody to ChAT.")
![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. DAPI counter stained.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunocytochemistry-Immunofluorescence-NB110-89724-img0020.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on paraffin sections of rat DRG using Rabbit antibody to ChAT.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Paraffin-NB110-89724-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. DAPI counter stained.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunocytochemistry-Immunofluorescence-NB110-89724-img0019.jpg)
![Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord (free floating sections) at 1:1000 dilution using Rabbit antibody to c-terminal region of ChAT (choline O-acetyltransferase): whole serum (NB110-89724).](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Free-Floating-NB110-89724-img0013.jpg)
![Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Free-Floating-NB110-89724-img0012.jpg)
![Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Free-Floating-NB110-89724-img0014.jpg)
![Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Free-Floating-NB110-89724-img0015.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on paraffin sections of rat DRG using Rabbit antibody to ChAT.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Paraffin-NB110-89724-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Staining Choline Acetyltransferase in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-JA67-11-Immunocytochemistry-Immunofluorescence-NBP2-66779-img0004.jpg "Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Staining Choline Acetyltransferase in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Analysis of paraffin-embedded rat stomach tissue using anti- Choline Acetyltransferase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-JA67-11-Immunohistochemistry-Paraffin-NBP2-66779-img0006.jpg)
![Flow Cytometry: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Analysis of N2A cells with Choline Acetyltransferase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-JA67-11-Flow-Cytometry-NBP2-66779-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Staining ChoLine Acetyltransferase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-JA67-11-Immunocytochemistry-Immunofluorescence-NBP2-66779-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Staining Choline Acetyltransferase in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-JA67-11-Immunocytochemistry-Immunofluorescence-NBP2-66779-img0003.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (JA67-11) [NBP2-66779] - Analysis of paraffin-embedded mouse brain tissue using anti- Choline Acetyltransferase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-JA67-11-Immunohistochemistry-Paraffin-NBP2-66779-img0002.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Choline Acetyltransferase/ChAT Antibody (3169) [NBP2-46650] - Staining in human placenta and liver tissues. Corresponding CHAT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3169-Immunohistochemistry-Paraffin-NBP2-46650-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Choline Acetyltransferase/ChAT Antibody (3169) [NBP2-46650] - Staining in human placenta and liver tissues. Corresponding CHAT RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of rat brain shows strong cytoplasmic positivity in acetylcholine neurons in the basal forebrain.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3169-Immunohistochemistry-NBP2-46650-img0017.jpg)
![Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of rat brain shows strong cytoplasmic positivity in acetylcholine neurons in the medial septum.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3169-Immunohistochemistry-NBP2-46650-img0018.jpg)
![Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of mouse brain shows strong cytoplasmic positivity in acetylcholine neurons in the basal forebrain.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3169-Immunohistochemistry-Paraffin-NBP2-46650-img0010.jpg)
![Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of mouse basal forebrain shows strong positivity in acetylcholine neurons in the caudate putamen.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3169-Immunohistochemistry-Paraffin-NBP2-46650-img0011.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of human cerebral cortex shows positivity in cholinergic neural fibers.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3169-Immunohistochemistry-Paraffin-NBP2-46650-img0012.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of human placenta shows strong cytoplasmic positivity in a subset of cells in chorionic villi.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3169-Immunohistochemistry-Paraffin-NBP2-46650-img0013.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3169-Immunohistochemistry-Paraffin-NBP2-46650-img0014.jpg)
![Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (8E7) [NBP3-26659] - Image of Choline Acetyltransferase/ChAT Antibody (8E7) diluted at 1:50 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.22% DAB.](http://images.novusbio.com/fullsize/nbp3-26659_rabbit-choline-acetyltransferase-chat-mab-8e7-26220241547564.jpg "Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (8E7) [NBP3-26659] - Image of Choline Acetyltransferase/ChAT Antibody (8E7) diluted at 1:50 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.22% DAB.")
![Western Blot: Choline Acetyltransferase/ChAT Antibody (8E7) [NBP3-26659] - Positive Western Blot detected in: Mouse Brain tissue lysate. All lanes: Choline Acetyltransferase/ChAT Antibody at 1: 500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 70 kDa Observed band size: 70 kDa](http://images.novusbio.com/fullsize/nbp3-26659_rabbit-choline-acetyltransferase-chat-mab-8e7-282202411442610.jpg)
![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (8E7) [NBP3-26659] - Staining of SH-SY5Y with Choline Acetyltransferase/ChAT Antibody (8E7) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 498-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26659_rabbit-choline-acetyltransferase-chat-mab-8e7-28220241323623.jpg)
![Flow Cytometry: Choline Acetyltransferase/ChAT Antibody (8E7) [NBP3-26659] - Overlay Peak curve showing SH-SY5Y cells stained with Choline Acetyltransferase/ChAT Antibody (8E7) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26659_rabbit-choline-acetyltransferase-chat-mab-8e7-282202414183675.jpg)
![Western Blot: Choline Acetyltransferase/ChAT Antibody (1I4V0) [NBP3-15621] - Western blot analysis of extracts of SH-SY5Y cells, using Choline Acetyltransferase/ChAT antibody (NBP3-15621) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-1I4V0-Western-Blot-NBP3-15621-img0002.jpg "Western Blot: Choline Acetyltransferase/ChAT Antibody (1I4V0) [NBP3-15621] - Western blot analysis of extracts of SH-SY5Y cells, using Choline Acetyltransferase/ChAT antibody (NBP3-15621) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Parafin-Choline Acetyltransferase/ChAT Antibody (1I4V0) [NBP3-15621] - Mouse brain using CHAT Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15621_rabbit-choline-acetyltransferase-chat-mab-1i4v0-immunohistochemistry-paraffin-2510202310736..jpg)
![Immunohistochemistry-Paraffin-Choline Acetyltransferase/ChAT Antibody (1I4V0) [NBP3-15621] - Rat brain using CHAT Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15621_rabbit-choline-acetyltransferase-chat-mab-1i4v0-immunohistochemistry-paraffin-251020231095..jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP2-33530] - Staining of human lymph node shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Paraffin-NBP2-33530-img0009.jpg "Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP2-33530] - Staining of human lymph node shows no positivity in lymphoid cells as expected.")
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP2-33530] - Staining of mouse basal forebrain shows strong cytoplasmic positivity in neuronal cells and fibers.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Paraffin-NBP2-33530-img0006.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP2-33530] - Staining of mouse cerebellum shows positivity in a subset of neural fiber endings.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Paraffin-NBP2-33530-img0007.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP2-33530] - Staining of human caudate nucleus shows strong cytoplasmic positivity in a large neurons.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Paraffin-NBP2-33530-img0008.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP2-33530] - Staining of human small intestine shows strong cytoplasmic positivity in neuroendocrine cells.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Paraffin-NBP2-33530-img0010.jpg)
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