![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Choline Acetyltransferase/ChAT Antibody (3169) [NBP2-46650] - Staining in human placenta and liver tissues. Corresponding CHAT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3169-Immunohistochemistry-Paraffin-NBP2-46650-img0015.jpg "Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Choline Acetyltransferase/ChAT Antibody (3169) [NBP2-46650] - Staining in human placenta and liver tissues. Corresponding CHAT RNA-seq data are presented for the same tissues.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | IHC |
| Clone | CL3169 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence GLFSSYRLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAEARTVLVKDSTN |
| Epitope | GLFSSYRLPGHTQDT |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CHAT |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Choline Acetyltransferase/ChAT Antibody (NBP2-46650)Find related products by research area.
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Choline Acetyltransferase (ChAT) – a useful Immunohistochemical marker for morphological studies of neurons Choline Acetyltransferase (ChAT) is the enzyme that is responsible for biosynthesis of the neurotransmitter acetylcholine. The majority of acetylcholine is synthesized locally at nerve terminals where ChAT catalyzes the transfer of an acetyl group ... Read full blog post. |
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![Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of rat brain shows strong cytoplasmic positivity in acetylcholine neurons in the basal forebrain.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3169-Immunohistochemistry-NBP2-46650-img0017.jpg "Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of rat brain shows strong cytoplasmic positivity in acetylcholine neurons in the basal forebrain.")
![Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of rat brain shows strong cytoplasmic positivity in acetylcholine neurons in the medial septum.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3169-Immunohistochemistry-NBP2-46650-img0018.jpg "Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of rat brain shows strong cytoplasmic positivity in acetylcholine neurons in the medial septum.")
![Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of mouse brain shows strong cytoplasmic positivity in acetylcholine neurons in the basal forebrain.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3169-Immunohistochemistry-Paraffin-NBP2-46650-img0010.jpg "Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of mouse brain shows strong cytoplasmic positivity in acetylcholine neurons in the basal forebrain.")
![Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of mouse basal forebrain shows strong positivity in acetylcholine neurons in the caudate putamen.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3169-Immunohistochemistry-Paraffin-NBP2-46650-img0011.jpg "Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of mouse basal forebrain shows strong positivity in acetylcholine neurons in the caudate putamen.")
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of human cerebral cortex shows positivity in cholinergic neural fibers.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3169-Immunohistochemistry-Paraffin-NBP2-46650-img0012.jpg "Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of human cerebral cortex shows positivity in cholinergic neural fibers.")
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of human placenta shows strong cytoplasmic positivity in a subset of cells in chorionic villi.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3169-Immunohistochemistry-Paraffin-NBP2-46650-img0013.jpg "Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of human placenta shows strong cytoplasmic positivity in a subset of cells in chorionic villi.")
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-CL3169-Immunohistochemistry-Paraffin-NBP2-46650-img0014.jpg "Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Staining of human liver shows no positivity in hepatocytes as expected.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")