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Choline Acetyltransferase/ChAT Antibody

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Western Blot: Choline Acetyltransferase Antibody [NB300-910] - Analysis of Choline Acetyltransferase in Human Placenta lysate using this antibody at 0.03 ug/ml.

Product Details

Summary
Reactivity Hu, Po, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Choline Acetyltransferase/ChAT Antibody Summary

Immunogen
Peptide with sequence CKEKATRPSQGHQP corresponding to C-Terminus according to NP_066264.3, NP_065574.3, NP_001136405.1.
Marker
Cholinergic Neuronal Marker
Specificity
This antibody is expected to recognise isoform 1 (NP_066264.3), isoform 2 (NP_065574.3) and isoform 3 (NP_001136405.1). Reported variants represent identical protein (NP_066264.3; NP_066265.3; NP_066266.3; NP_001136406.1; NP_001136401.1).
Predicted Species
Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CHAT
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.03 - 0.3 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in human placenta lysates (calculated MW of 70.5 kDa band according to NP_065574 and NP_066264).
Publications
Read Publication using NB300-910.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Choline Acetyltransferase/ChAT Antibody

  • acetyl CoA:choline O-acetyltransferase
  • CHAT
  • CHOACTase
  • Choline acetylase
  • Choline Acetyltransferase
  • choline O-acetyltransferase
  • CMS1A
  • CMS1A2
  • EC 2.3.1
  • EC 2.3.1.6

Background

Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Choline Acetyltransferase/ChAT Antibody (NB300-910)(1)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Choline Acetyltransferase/ChAT.

Choline Acetyltransferase (ChAT) – a useful Immunohistochemical marker for morphological studies of neurons
Choline Acetyltransferase (ChAT) is the enzyme that is responsible for biosynthesis of the neurotransmitter acetylcholine. The majority of acetylcholine is synthesized locally at nerve terminals where ChAT catalyzes the transfer of an acetyl group ...  Read full blog post.

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Bioinformatics

Gene Symbol CHAT
Entrez
Uniprot