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![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0009.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ACAT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar pattern to independent antibody NBP1-89285 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-89284] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89284-img0007.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum shows moderate positivity in mitochondria in Purkinje cells.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0014.jpg)
![Western Blot: ACAT1 Antibody [NBP1-89284] - ACAT1 porcine cerebral cortex. Image from verified customer review.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0008.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human testis shows strong positivity in mitochondria in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0011.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human kidney shows strong positivity in mitochondria in cells in tubules.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0012.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human liver shows strong positivity in mitochondria in hepatocytes.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-46140] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp1-46140_goat-polyclonal-acat1-antibody-imgenex-img-31254-16520239183319.jpg "Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-46140] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Western Blot: ACAT1 Antibody [NBP1-46140] - (0.01ug/ml) staining of Human (A), and (0.1ug/ml) Mouse (B) and Rat (C), Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp1-46140_goat-polyclonal-acat1-antibody-imgenex-img-31254-16520239183320.jpg)
![Flow Cytometry: ACAT1 Antibody [NBP1-46140] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp1-46140_goat-polyclonal-acat1-antibody-imgenex-img-31254-16520239183336.jpg)
![Western Blot: ACAT1 Antibody [NBP1-46140] - (0.5ug/ml) staining of KNRK (A) and MCF7 (B), and (0.1ug/ml) HepG2 (C), Caco-2 (D), HEK293 (E), and NIH3T3 (F) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp1-46140_goat-polyclonal-acat1-antibody-imgenex-img-31254-16520239183337.jpg)
![Western Blot: ACAT1 Antibody [NBP1-51994] - Analysis of Mouse (A) and Rat (B) kidney lysates, and Mouse (C) and Rat (D) Liver lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-51994-img0003.jpg "Western Blot: ACAT1 Antibody [NBP1-51994] - Analysis of Mouse (A) and Rat (B) kidney lysates, and Mouse (C) and Rat (D) Liver lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: ACAT1 Antibody [NBP1-51994] - (0.01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-51994-img0002.jpg)
![Western Blot: ACAT1 Antibody (15E5) [NBP2-22565] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hep3B cell lysate Lane 2. : 293T cell lysate Lane 3. : A431 cell lysate.](http://images.novusbio.com/fullsize/ACAT1-Antibody-15E5-Western-Blot-NBP2-22565-img0003.jpg "Western Blot: ACAT1 Antibody (15E5) [NBP2-22565] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hep3B cell lysate Lane 2. : 293T cell lysate Lane 3. : A431 cell lysate.")
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody (15E5) [NBP2-22565] - Analysis of ACAT1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACAT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/ACAT1-Antibody-15E5-Immunocytochemistry-Immunofluorescence-NBP2-22565-img0002.jpg)
![Western Blot: ACAT1 Antibody (15E5) [NBP2-22565] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/ACAT1-Antibody-15E5-Western-Blot-NBP2-22565-img0001.jpg)
![Western Blot: ACAT1 Antibody (S02-5G8) [NBP3-19555] - Western blot detection of ACAT1 in K562, Hela cell lysates using NBP3-19555 (1:1000 diluted). Predicted band size: 45kDa. Observed band size: 45kDa.](http://images.novusbio.com/fullsize/ACAT1-Antibody-S02-5G8-Western-Blot-NBP3-19555-img0001.jpg "Western Blot: ACAT1 Antibody (S02-5G8) [NBP3-19555] - Western blot detection of ACAT1 in K562, Hela cell lysates using NBP3-19555 (1:1000 diluted). Predicted band size: 45kDa. Observed band size: 45kDa.")
![SDS-Page: Recombinant Human ACAT1 Protein [NBP2-22664]](http://images.novusbio.com/fullsize/Recombinant-Human-ACAT1-Protein-SDS-Page-NBP2-22664-img0002.jpg "SDS-Page: Recombinant Human ACAT1 Protein [NBP2-22664]")
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89285 (A) shows similar protein distribution across tissues to independent antibody NBP1-89284 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89285-img0011.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89285 (A) shows similar protein distribution across tissues to independent antibody NBP1-89284 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: ACAT1 Antibody [NBP1-89285] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-ACAT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89285-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ACAT1 Antibody [NBP1-89285] - Analysis using Anti-ACAT1 antibody NBP1-89285 (A) shows similar pattern to independent antibody NBP1-89284 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89285-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-89285] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89285-img0009.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human testis shows strong positivity in mitochondria in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89285-img0016.jpg)
![Western Blot: ACAT1 Antibody [NBP1-89285] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89285-img0008.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human cerebellum shows strong positivity in mitochondria in purkinje cells.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89285-img0013.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human liver shows strong positivity in mitochondria in hepatocytes.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89285-img0014.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human kidney shows strong positivity in mitochondria in cells in tubules.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89285-img0015.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: ACAT1 Antibody [NBP1-89285] - ACAT1 protein expression in wild-type and acat-1-/- macrophages. Cell lysates were immunoblotted and ACAT1 protein levels were compared with GAPDH as loading control. CRISPR/Cas-9 used to knockout acat-1. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29390006/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Knockout-Validated-NBP1-89285-img0017.jpg)
![Western Blot: ACAT1 Antibody [NBP1-89285] - Blocking LD formation increases C. burnetii growth.Wild-type C. burnetii growth in infected MH-S cells treated with different inhibitors was measured at 2 & 4 days post-infection by FFU assay. A-D) Representative images for wild-type MH-S macrophages treated with inhibitors, fixed, stained for PLIN2 (LDs; green) & C. burnetii (red) & imaged day 4 post-treatment at 100X. Scale bar = 10 µm. E) Growth while inhibiting LD formation with triacsin C (10 µM) in wild-type MH-S macrophages. Error bars represent the mean of 4 independent experiments +/- SEM. ** = p <0.01 compared to vehicle-treated cells as determined by two-way ANOVA with Bonferroni post-hoc test. F) ACAT1 protein expression in wild-type & acat-1-/- macrophages. Cell lysates were immunoblotted & ACAT1 protein levels were compared with GAPDH as loading control. G) C. burnetii growth in vehicle-treated wild-type & acat-1-/- MH-S macrophages & (H) T863-treated acat-1-/- MH-S macrophages. Error bars represent the mean of at least 3 independent experiments +/- SEM., * = p<0.05, *** = p <0.001 as determined by two-way ANOVA with Bonferroni post-hoc test. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0192215), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89285_rabbit-polyclonal-acat1-antibody-310202416121928.jpg)