Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712

Name: Delta Np73 Antibody (38C674.2)
Brand: Novus Biologicals
SKU: NBP2-24873
Price: 399 USD

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Accepted Answer

These antibodies are all purified from tissue culture supernatant.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, ICC/IF, IHC, IP, ChIP
Clone	38C674.2
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	1.0 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP2-24873AF350

Alexa Fluor 405 NBP2-24873AF405

Alexa Fluor 488 NBP2-24873AF488

Alexa Fluor 532 NBP2-24873AF532

Alexa Fluor 594 NBP2-24873AF594

Alexa Fluor 647 NBP2-24873AF647

Alexa Fluor 700 NBP2-24873AF700

Alexa Fluor 750 NBP2-24873AF750

Biotin NBP2-24873B

DyLight 350 NBP2-24873UV

DyLight 405 NBP2-24873V

DyLight 488 NBP2-24873G

DyLight 550 NBP2-24873R

DyLight 594 NBP2-24873DL594

DyLight 650 NBP2-24873C

DyLight 680 NBP2-24873FR

DyLight 755 NBP2-24873IR

FITC NBP2-24873F

HRP NBP2-24873H

Janelia Fluor 549 NBP2-33262JF549

Janelia Fluor 646 NBP2-33262JF646

Catalog# & Formulation	Size	Price
Azide and BSA Free NBP2-33262

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24873AF350
Alexa Fluor 405	NBP2-24873AF405
Alexa Fluor 488	NBP2-24873AF488
Alexa Fluor 532	NBP2-24873AF532
Alexa Fluor 594	NBP2-24873AF594
Alexa Fluor 647	NBP2-24873AF647
Alexa Fluor 700	NBP2-24873AF700
Alexa Fluor 750	NBP2-24873AF750
Biotin	NBP2-24873B
DyLight 350	NBP2-24873UV
DyLight 405	NBP2-24873V
DyLight 488	NBP2-24873G
DyLight 550	NBP2-24873R
DyLight 594	NBP2-24873DL594
DyLight 650	NBP2-24873C
DyLight 680	NBP2-24873FR
DyLight 755	NBP2-24873IR
FITC	NBP2-24873F
HRP	NBP2-24873H
Janelia Fluor 549	NBP2-33262JF549
Janelia Fluor 646	NBP2-33262JF646

Delta Np73 Antibody (38C674.2)

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Publications for Delta Np73 Antibody (NBP2-24873)(41)

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Immunogen	This antibody was developed against a peptide corresponding to a portion of amino acid residues 1-50 of human dNp73. Mouse and human sequences are more than 95 % identical at these amino acid residues. In mouse, alanine at position 8 is replaced by methionine.
Isotype	IgG1 Kappa
Clonality	Monoclonal
Host	Mouse
Gene	TP73
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.05% Sodium Azide
Concentration	1.0 mg/ml
Purity	Protein G purified

Publications using NBP2-24873	Applications	Species
Zhao L, Hsiao T, Stonesifer C et al. The Robust Tumoricidal Effects of Combined BET/HDAC Inhibition in CTCL Can Be Reproduced by delta Np73 Depletion The Journal of investigative dermatology 2022-07-01 [PMID: 35787399] (Human)		Human
Malik N, Yan H, Yang HH et al. CBFB cooperates with p53 to maintain TAp73 expression and suppress breast cancer PLoS genetics 2021-05-01 [PMID: 33945523] (WB, Human)	WB	Human
Guo J, Jia R Overexpression of splicing factor poly (rC)-binding assets.researchsquare.com 2020-01-01 [PMID: 30132844] (WB, Human)	WB	Human
Veselska R, Neradil J, Nekulova M et al. Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies. Histol Histopathol. 2013-07-01 [PMID: 23338942]
Pham T Fuzzy Recurrence Plots and Networks with Applications in Biomedicine Fuzzy Recurrence Plots and Networks with Applications in Biomedicine 2020-01-08 (IF/IHC)	IF/IHC
Pham TD, Fan C, Pfeifer D et al. Image-Based Network Analysis of DNp73 Expression by Immunohistochemistry in Rectal Cancer Patients Front Physiol 2019-01-08 [PMID: 31969833] (IF/IHC, Human)	IF/IHC	Human
Gonzalez R, De la Rosa AJ, Rufini A et al. Role of p63 and p73 isoforms on the cell death in patients with hepatocellular carcinoma submitted to orthotopic liver transplantation. PLoS ONE. 2017-03-28 [PMID: 28350813] (ICC/IF, Human)	ICC/IF	Human
Morandi EM, Verstappen R, Zwierzina ME et al. ITGAV and ITGA5 diversely regulate proliferation and adipogenic differentiation of human adipose derived stem cells Sci Rep 2016-07-01 [PMID: 27363302] (WB, Human)	WB	Human
Prabhu VV, Hong B, Allen JE et al. Small molecule prodigiosin restores p53 tumor suppressor activity in chemoresistant colorectal cancer stem cells via c-Jun-mediated deltaNp73 inhibition and p73 activation. Cancer Res. 2016-01-12 [PMID: 26759239] (WB, Human)	WB	Human
Hassan HM, Dave BJ, Singh RK et al. TP73, an under-appreciated player in non-Hodkin lymphoma pathogenesis and managmenet. Curr. Mol. Med. 2014-05-01 [PMID: 24730526] (IHC-P, Human) Details: Follicular hyperplasia and follicular lymphoma, Fig 3. Delta Np73 expression was higher in follicular lymphoma than follicular hyperplasia. The Delta Np73 mAb was used at 0.5 ug/ml for IHC-P.	IHC-P	Human
Hassan HM, Varney ML, Jain S et al. Disruption of chromosomal locus 1p36 differentially modulates TAp73 and Delta Np73 expression in follicular lymphoma. Leuk. Lymphoma 2014-03-25 [PMID: 24660851] (IHC-P, Human) Details: Follicular ymphoma: the expression of delta Np73 isoforms was observed in activated and germinal center B cells.	IHC-P	Human
Shin Aesun, Joo Jungnam, Bak Jeongin et al. Site-specific risk factors for colorectal cancer in a Korean population. PLoS One. 2011-01-01 [PMID: 21853085] (ICC/IF, Human)	ICC/IF	Human
Rastogi S, Rizwani W, Joshi B et al. TNF-alpha response of vascular endothelial and vascular smooth muscle cells involve differential utilization of ASK1 kinase and p73. Cell Death Differ. 2012-02-01 [PMID: 21738216] (WB, Human) Details: See Figure 4 where this antibody, Delta Np73 Antibody (38C674.2), was used in WB with HAEC	WB	Human
Leonard M K, Kommagani R, Payal V et al. DeltaNp63alpha regulates keratinocyte proliferation by controlling PTEN expression and localization. Cell Death Differ. 2011-12-01 [PMID: 21637289]
Accardi R, Scalise M, Gheit T et al. IkappaB kinase beta promotes cell survival by antagonizing p53 functions through DeltaNp73alpha phosphorylation and stabilization. Mol Cell Biol. 2011-06-01 [PMID: 21482671]
Nekulova M, Zitterbart K, Sterba J, Veselska R. Analysis of the intracellular localization of p73 N-terminal protein isoforms TAp73 and deltaNp73 in medulloblastoma cell lines. J Mol Histol. 2010-10-01 [PMID: 20803057]
Vilgelm AE, Washington MK, Wei J et al. Interactions of the p53 protein family in cellular stress response in gastrointestinal tumors Mol Cancer Ther 2010-03-04 [PMID: 20197393] Details: Citation using the Non-Recombinant Monoclonal and Biotin format of this antibody.
Wilhelm MT, Rufini A, Wetzel MK et al. Isoform-specific p73 knockout mice reveal a novel role for delta Np73 in the DNA damage response pathway. Genes Dev. 2010-03-15 [PMID: 20194434]
Lefkimmiatis Konstantinos, Caratozzolo Mariano Francesco, Merlo Paola et al. p73 and p63 sustain cellular growth by transcriptional activation of cell cycle progression genes. Cancer Res. 2009-11-15 [PMID: 19861536] (WB, Human)	WB	Human
Rosenbluth JM, Johnson K, Tang L et al. Evaluation of p63 and p73 antibodies for cross-reactivity. Cell Cycle. 2009-11-15 [PMID: 19855172]
Sari Youssef, Smith Kathryn D, Ali Pir K, Rebec George V. Upregulation of GLT1 attenuates cue-induced reinstatement of cocaine-seeking behavior in rats. J Neurosci. 2009 Jul 22 [PMID: 19625514] (IP)	IP
Marques-Garcia F, Ferrandiz N, Fernandez-Alonso R et al. p73 plays a role in erythroid differentiation through GATA1 induction. J Biol Chem. 2009-08-07 [PMID: 19509292] (Chemotaxis) Details: ChIP in k562 cells (Figs 4C, 6) and fetal liver extracts from mouse embryos (Fig 10): 1. p73 (IMG-246) 2. p73 [deltaNp73 (IMG-313)]	Chemotaxis
Tomasini R, Tsuchihara K, Wilhelm M et al. TAp73 knockout shows genomic instability with infertility and tumor suppressor functions. Genes Dev. 2008-10-01 [PMID: 18805989] (WB, Mouse) Details: WB (mouse embryonic fibroblasts), Fig. 1D.	WB	Mouse
Rosenbluth Jennifer M, Mays Deborah J, Pino Maria F et al. A gene signature-based approach identifies mTOR as a regulator of p73. Mol Cell Biol. 2008-10-01 [PMID: 18678646] (Human)		Human
Lunghi P, Giuliani N, Mazzera L et al. Targeting MEK/MAPK signal transduction module potentiates ATO-induced apoptosis in multiple myeloma cells through multiple signaling pathways. Blood. 2008-09-15 [PMID: 18583568] (WB, Human) Details: 1. p73 (IMG-246): WB (human myeloma cell lines (HMCLs) XG-6 and XG-1), Fig. 4Ci. 2. p73 (deltaNp73) [IMG-313A]: WB (HMCLs), Fig. 4Ai. 3. DcR2 (IMG-121): WB (HMCLs), Fig. 5A.	WB	Human
Mor I, Bruck T, Greenberg D et al. Alternate AChE-R variants facilitate cellular metabolic activity and resistance to genotoxic stress through enolase and RACK1 interactions. Chem Biol Interact. 2008-09-25 [PMID: 18572152] Details: deltaNp73 (IMG-313A), tankyrase (IMG-146). WB: GFP expressing CHO cell line, Fig 3E.
Cancino GI, Toledo EM, Leal NR et al. STI571 prevents apoptosis, tau phosphorylation and behavioural impairments induced by Alzheimer's beta-amyloid deposits. Brain. 2008-09-01 [PMID: 18559370] (WB, Rat) Details: Antibodies cited [WB (rat hippocampal cells)]: 1. IMG-246 (p73): Fig. 5A. 2. IMG-313 (delta-Np73): Fig. 5B.	WB	Rat
Righetti SC, Perego P, Carenini N, Zunino F. Cooperation between p53 and p73 in cisplatin-induced apoptosis in ovarian carcinoma cells. Cancer Lett. 2008-05-08 [PMID: 18261847] (WB, Human) Details: WB (human ovarian cell lines A2780 and A2780/BBR3464), Fig. 3.	WB	Human
Mor I, Sklan EH, Podoly E et al. Acetylcholinesterase-R increases germ cell apoptosis but enhances sperm motility. J Cell Mol Med. 2008-04-01 [PMID: 18194455] Details: Antibodies cited [WB (S cerevisiae)]: 1. IKKa [IMG-136A], Figs. 1a, 2b, 3a, 3d (TNF-stimulated HeLa cells), Fig. 3a IP (S cerevisiae), Figs. 3a, 3c, 3d IP (TNF-stimulated HeLa cells), Figs. 3a, 3c 2. IKKb [IMG-129A], Figs. 1a, 1c, 2b, 3a, 3c, 3e, 3f, 3g,
Zitterbart K, Zavrelova I, Kadlecova J et al. p73 expression in medulloblastoma: TAp73/DeltaNp73 transcript detection and possible association of p73alpha/DeltaNp73 immunoreactivity with survival. Acta Neuropathol. 2007-12-01 [PMID: 17912537]
Castellino Robert C, De Bortoli Massimiliano, Lin Linda L et al. Overexpressed TP73 induces apoptosis in medulloblastoma. BMC Cancer. 2007-01-01 [PMID: 17626635] (Human)		Human
Toscano F, Parmentier B, Fajoui ZE et al. p53 dependent and independent sensitivity to oxaliplatin of colon cancer cells. Biochem Pharmacol. 2007-08-01 [PMID: 17559811] (WB) Details: Antibodies cited: deltaNp73 (IMG-313A) [WB, Fig 9 (oxaliplatin treated HCT116, Isreco1, and V9P cell lines)].	WB
Bozzetti C, Nizzoli R, Musolino A et al. p73 and p53 pathway in human breast cancers. J Clin Oncol. 2007-04-10 [PMID: 17416871]
Cabrera-Socorro A, Pueyo Morlans M, Suarez Sola ML et al. Multiple isoforms of the tumor protein p73 are expressed in the adult human telencephalon and choroid plexus and present in the cerebrospinal fluid. Eur J Neurosci. 2006-04-01 [PMID: 16630058]
Dominguez G, Garcia JM, Pena C et al. DeltaTAp73 upregulation correlates with poor prognosis in human tumors: putative in vivo network involving p73 isoforms, p53, and E2F-1. J Clin Oncol. 2006-02-10 [PMID: 16380414] (IHC-Fr, Human) Details: Antibodies cited [IHC (F) in human colon and breast tumors], Fig 2: 1. p73 (IMG-313A) 2. p73 (IMG-246).	IHC-Fr	Human
Saifudeen Z, Diavolitsis V, Stefkova J et al. Spatiotemporal switch from DeltaNp73 to TAp73 isoforms during nephrogenesis: impact on differentiation gene expression. J Biol Chem. 2005-06-17 [PMID: 15805112] (WB, IHC-P, Rat) Details: deltaNp73 (IM-313A) [IHC (paraffin), Fig 5 (adult rat kindey)], [WB, Fig 5 (adult rat kidney)].	WB, IHC-P	Rat
Sayan AE, Paradisi A, Vojtesek B et al. New antibodies recognizing p73: comparison with commercial antibodies. Biochem Biophys Res Commun. 2005-04-29 [PMID: 15781249] (ICC/IF, WB, Human) Details: Antibodies cited (p73 transfected cells, SK-N-SH human neuroblastoma cells, HaCaT human keratinocyte cells): 1. p73 (IMG-246): IP, Fig 3B (p73 transfected cells); WB, Figs 1 and 2 (p73 transfected cells). 2. p73 (IMG-259A): WB, Figs 1 and 2 (p73 transfect	ICC/IF, WB	Human
Papoutsaki M, Lanza M, Marinari B et al. The p73 gene is an anti-tumoral target of the RARbeta/gamma-selective retinoid tazarotene. J Invest Dermatol. 2004-12-01 [PMID: 15610529] (WB) Details: Monoclonal antibodies cited for IHC (Bouin's) in human breast cancer, Fig 1: 1. IL3-Ra/CD123, clone 107D2.08: Cat no DDX0300 2. CD207/Langerin, clone 808E10.01: Cat no DDX0361 3. CD208/DC-LAMP, clone 101E1.01: Cat no DDX0191 4. CD303/BCDA-2, clone 104C12.08: Cat no DDX0041.	WB
Tomkova K, Belkhiri A, El-Rifai W, Zaika AI. p73 isoforms can induce T-cell factor-dependent transcription in gastrointestinal cells. Cancer Res. 2004-09-15 [PMID: 15374945] (WB) Details: Antibodies cited: deltaNp73 (IMG-313A) [WB, no data present (AGS gastric cell line)].	WB
Lunghi P, Costanzo A, Levrero M, Bonati A. Treatment with arsenic trioxide (ATO) and MEK1 inhibitor activates the p73-p53AIP1 apoptotic pathway in leukemia cells. Blood. 2004-07-15 [PMID: 15031205] (WB) Details: 1. p73 (IMG-259A) [WB, Fig 1CD, Fig 2B (NB4 acute promyelocytic and k562 erythroleukemia cells)]. 2. deltaNp73 (IMG-313A) [WB, Fig 1CD, Fig 2B (NB4 and k562 cells)].	WB
Vossio S, Palescandolo E, Pediconi N et al. DN-p73 is activated after DNA damage in a p53-dependent manner to regulate p53-induced cell cycle arrest. Oncogene. 2002-05-23 [PMID: 12032848] (WB, Human) Details: deltaNp73(IMG-313A) [WB, Fig 2B, Fig 4B (human osteosarcoma U2OS cells)].	WB	Human
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Gene Symbol	TP73
Uniprot	O15350(Human)