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Myosin Phosphatase Antibody

Images

 
Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-75097] - (4ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunocytochemistry/ Immunofluorescence: Myosin Phosphatase Antibody [NBP2-75097] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) ...read more
Flow Cytometry: Myosin Phosphatase Antibody [NBP2-75097] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa ...read more
Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-75097] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.
Immunocytochemistry/ Immunofluorescence: Myosin Phosphatase Antibody [NBP2-75097] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Myosin Phosphatase Antibody Summary

Immunogen
Peptide with sequence C-REDEYKQKYSRTYD, from the C Terminus of the protein sequence according to NP_002471.1, NP_001231919.1, NP_001231921 .1, NP_001137358.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PPP1R12A
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 4 - 5 ug/ml
  • Peptide ELISA 1:16000
Theoretical MW
115 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
TBS (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Antigen Affinity-purified

Alternate Names for Myosin Phosphatase Antibody

  • MBSmyosin phosphatase, target subunit 1
  • MGC133042
  • Myosin phosphatase target subunit 1
  • Myosin phosphatase-targeting subunit 1
  • MYPT1protein phosphatase 1 regulatory subunit 12A
  • protein phosphatase 1, regulatory (inhibitor) subunit 12A
  • Protein phosphatase myosin-binding subunit

Background

The major protein phosphatase-1 (PP1) is composed of 3 subunits with apparent molecular mass of 110-130, 37 & 20 kDa. The 110-130 kDa component act as a regulatory subunit known as myosine binding (MBS) or myosine phosphatae targeting subunit (MYPT). The N-terminal portion of MYPT binds to both PP1c-delta and phosphorylated myosin, thereby increasing myosin phosphatase activity. Two isoform of MYPT have been identified (MYPT1 & MYPT2) and share 61% sequence identity. While MYPT1 is widely distributed in human tissues, MYPT2 is mostly detected in brain and heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PPP1R12A