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Recombinant Human FGF-4 Protein

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SDS-Page: Human FGF-4 Protein [NBP2-34864]
SDS-Page: Human FGF-4 Protein [NBP2-34864]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier-Free
NBP2-34864-1mg
Carrier-Free
NBP2-34864-100ug
Carrier-Free
NBP2-34864-500ug
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Recombinant Human FGF-4 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 182 amino acids corresponding to FGF-4 Source: E. coli

Uniprot ID: P08620

Amino Acid Sequence: GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL
Details of Functionality
Fibroblast Growth Factor 4 protein is fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
FGF4
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of FGF-4 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
19.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-34864 in the following applications:
Publications
Read Publication using NBP2-34864.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, 350 mM NaCl, 0.02% Tween-20, pH 7.0.
Preservative
5% Trehalose
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Alternate Names for Recombinant Human FGF-4 Protein

  • FGF4
  • FGF-4
  • fibroblast growth factor 4
  • HBGF-4
  • HBGF-4Transforming protein KS3
  • heparin secretory transforming protein 1
  • Heparin secretory-transforming protein 1
  • Heparin-binding growth factor 4
  • HST-1
  • HST-1HSTF-1
  • HSTF1fibroblast growth factor 4 splice isoform
  • HSTFGF-4
  • human stomach cancer, transforming factor from FGF-related oncogene
  • kaposi sarcoma oncogene
  • KFGF
  • K-FGF
  • KS3
  • oncogene HST

Background

FGF4, also named FGFK and KFGF, belongs to the fibroblast growth factor (FGF) family. By signaling through the FGF R1c, 2c, 3c and 4 receptors, FGF4 has functions that maintain a population of progenitor cells in the epiblast that generates mesoderm, and contribute to the stem cell population that is incorporated in the tailbud. It is also required for axial elongation of the mouse embryo after gastrulation. Mature human FGF4 (71-206 a.a.) shares 91 %, 82 %, 94 % and 91 % a.a. identity with murine, rat, canine and bovine FGF4. Additionally, FGF4 shares about 30 % sequence identity with the prototypical members of the FGF family.)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Human
FGF-4 Protein
NBP2-34864
Recombinant Human FGF-4 Protein
Species: Hu
Applications: PAGE, Bioactivity

Publications for FGF-4 Recombinant Protein (NBP2-34864)(1)


Review for FGF-4 Recombinant Protein (NBP2-34864) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBP2-34864:
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SDS-PAGE Control
(1)
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Images Ratings Applications Species Date Details
SDS-PAGE Control FGF-4 NBP2-34864
Enlarge 		5
reviewed by:
Verified Customer
 SDS-PAGE Control 01/04/2017
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Summary

ApplicationSDS-PAGE Control
CommentsI ranked this protein with 5 stars because the cell differentiation was successfully performed.

Comments

CommentsSDS PAGE gel with Coomassie blue staining showing detection of Human FGF-4 Protein (NBP2-34864-25 ug) (3 ug).

FAQs for FGF-4 Recombinant Protein (NBP2-34864) (0)

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Recent Reviews

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SDS-PAGE Control FGF-4 NBP2-34864
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 Verified Customer
01/04/2017
Application: SDS-PAGE Control
Species:
read this review

Bioinformatics

Gene Symbol FGF4
Uniprot