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Erythropoietin R Antibody - BSA Free

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Western Blot: Erythropoietin R Antibody [NBP1-19388] - Western blot analysis of K562 (left) and SH-SY5Y (right) cell lysate using EPO receptor antibody at a concentration of 2 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

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Reviews & Publications
Protocols & FAQs
Support & Research

Erythropoietin R Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal portion of the human EPO receptor protein (between residues 300-400) [UniProt P19235]
Localization
Membrane
Specificity
EpoR (A364) detects endogenous levels of EpoR protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EPOR
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Simple Western 1:10
  • Western Blot 2.0 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-19388 in the following applications:

Publications
Read Publications using
NBP1-19388 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34552120).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Erythropoietin R Antibody - BSA Free

  • EpoR
  • EPO-R
  • Erythropoietin R
  • erythropoietin receptor
  • MGC138358

Background

The erythropoietin receptor (EPOR) is a cytokine receptor family member. There are three known isoforms of the receptor, EPOR-F (full length), EPOR-S (soluble form), and EPOR-T (truncated form). When bound to erythropoietin (EPO), EPOR is phosphorylated by Jak2 tyrosine kinase. Phosphorylated EPOR serves as the docking site for a number of different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. EPOR-Stat5 signaling appears to play a role in erythroid cell survival. Animal studies have shown that truncated EPOR that can still be bound by Jak2 are viable, suggesting that basal Jak2 activity is sufficient for erythropoiesis Stat5 binding is important, as a mutated Tyr343 on EPOR results in anemia and poor response to hypoxic stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Verified Customer
10/05/2022
Application: WB
Species: Human

zhang meng
11/17/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol EPOR
Entrez
Uniprot