Myosin

Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two isoforms that are highly and ubiquitously expressed in the cell.  For this reason, measuring beta-actin levels has served as a useful control in research experiments in order to have a baseline of protein expression to compare cell manipulations to.  However, beta actin has other implications in scientific research aside from acting

Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to validate experiments in the Sordaria macrospora.  In addition, the growth and morphogenesis of filamentous fungi is dependent on actin organization.  Actin is a very abundant protein across biological species and can transition between monomeric and filamentous states.  Actin has an alpha and beta isoform, both of which sha

The Myosin superfamily is a large and diverse protein family, and its members are grouped into many classes, are involved in a number of cellular pathways (1). Myosins contain actin- and ATP-binding sites in their conserved catalytic head domain and localize to a number of intracellular compartments and participate in many trafficking and anchoring events.

Myosin is a super family of actin based molecular motors that hydrolyze ATP and generate physical force to move specific molecules inside the cell. This super family, divided into at least twenty four classes based on head domain sequence similarity and domain organization^. The processivity of myosins along an actin filament and transport of intracellular ‘cargo’ are achieved by generating physical force from chemical energy of ATP followed by appropriate conformational changes (1).

Myosin is a well-known, hexameric molecular motor that is a key cytoskeletal component. It consists of a pair of myosin heavy chain subunits (MHC), a pair of essential myosin light chain subunits (MLC), and a pair of regulatory light chain subunits (RLCs).