![Western Blot: TRIM3/BERP Antibody [NB100-2494] - Staining of Human Cerebellum (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TRIM3-BERP-Antibody-Western-Blot-NB100-2494-img0003.jpg "Western Blot: TRIM3/BERP Antibody [NB100-2494] - Staining of Human Cerebellum (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Bv, CaSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence AKREDSPGPEVQP-C corresponding to N-Terminus according to NP_150594.2. |
| Specificity | Both variants (NP_006449.2; NP_150594.2;) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein. |
| Predicted Species | Canine (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | TRIM3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Please note the HEK cell experiements were performed using a previous batch (different goat). WB: Approx. 85 kDa band observed in mouse brain and human brain (cerebellum) lysate (predicted MW of approx. 80.8 kDa band according to NP_006449.2). |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TRIM3 |
| Entrez | |
| Uniprot |
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![Western Blot: TRIM3/BERP Antibody [NB100-2494] - HEK293 overexpressing TRIM3 and probed with (mock transfection in first lane).](http://images.novusbio.com/fullsize/TRIM3-BERP-Antibody-Western-Blot-NB100-2494-img0001.jpg "Western Blot: TRIM3/BERP Antibody [NB100-2494] - HEK293 overexpressing TRIM3 and probed with (mock transfection in first lane).")
![Western Blot: TRIM3/BERP Antibody [NB100-2494] - Western blot analysis of TRIM3/BERP in Mouse Brain lysate (RIPA buffer, 30ug total protein per lane) using NB100-2494 at 0.05ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/TRIM3-BERP-Antibody-Western-Blot-NB100-2494-img0002.jpg "Western Blot: TRIM3/BERP Antibody [NB100-2494] - Western blot analysis of TRIM3/BERP in Mouse Brain lysate (RIPA buffer, 30ug total protein per lane) using NB100-2494 at 0.05ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
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