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472 Results for "novus"
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MAB8214

FoxP3 Antibody (1054C) [Unconjugated]

(1 Review)
(19 Publications)
100 μg
MAB8214
Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) stimulated to induce Regulatory T Cells (Tregs) with Recombinant Human TGF‑ beta 1 (Catalog # 240-B) and Recombinant Human IL‑2 (Catalog # 202-IL) for 2 days were stained with Rabbit Anti-Human/Mouse FoxP3 Monoclonal Antibody (Catalog # MAB8214) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Hu, Mu
Species Glossary
Reactivity: Hu, Mu
Flow, IHC, Dual ISH-IHC, ICC/IF
Applications: Flow, IHC, Dual ISH-IHC, ICC/IF
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
         
Validated by:          
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 IC8214U-100UG
Alexa Fluor 405 IC8214V-100UG
Alexa Fluor 488 IC8214G-100
Alexa Fluor 532 FAB8214X
Alexa Fluor 594 IC8214T-100UG
Alexa Fluor 647 IC8214R-100UG
Alexa Fluor 700 IC8214N-100
Alexa Fluor 750 IC8214S-100UG
Allophycocyanin IC8214A-100
Allophycocyanin FPK8214A-025
Allophycocyanin FPK8214A-100
Allophycocyanin/Cy7 FAB8214APCCY7
Biotin FAB8214B
DyLight 350 FAB8214D
DyLight 405 FAB8214E
DyLight 488 FAB8214K
DyLight 550 FAB8214L
DyLight 594 FAB8214M
DyLight 650 FAB8214W
DyLight 680 FAB8214Y
DyLight 755 FAB8214Z
FITC FAB8214F
HRP FAB8214H
Janelia Fluor 549 FAB8214I
Janelia Fluor 646 FAB8214J
PE/Atto594 FAB8214PEATT594
PE/Cy5.5 FAB8214PECY55
PE/Cy7 FAB8214PECY7
PerCP FAB8214C
Phycoerythrin FPK8214P-100
Phycoerythrin FPK8214P-025
Phycoerythrin IC8214P-100
mFluor Violet 450 SE FAB8214MFV450
mFluor Violet 500 SE FAB8214MFV500
mFluor Violet 610 SE FAB8214MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free IC8214P-100
Carrier Free IC8214A-100
Carrier Free IC8214G-100
Carrier Free IC8214N-100
Carrier Free FPK8214A-025
Carrier Free FPK8214A-100
Carrier Free FPK8214P-025
Carrier Free FPK8214P-100
Carrier Free MAB8214-SP
Carrier Free IC8214V-100UG
Carrier Free IC8214U-100UG
Carrier Free IC8214T-100UG
Carrier Free IC8214S-100UG
Carrier Free IC8214R-100UG
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MAB1205

JNK1/2/3 [p Thr183, p Tyr185] Antibody (1006A) [Unconjugated]

(1 Review)
(8 Publications)
100 μg
MAB1205
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and 293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2 ultraviolet light (UV) followed by a 30 minute recovery. PVDF membrane was probed with 1 μg/ml of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Monoclonal Antibody (Catalog # MAB1205), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-JNK (T183/Y185) at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Simple Western, ICC/IF
Applications: WB, Simple Western, ICC/IF
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB1205-SP
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F0102B

Goat anti-Mouse IgG Secondary Antibody [Phycoerythrin]

(12 Publications)
100 Tests
F0102B
Human peripheral blood granulocytes were stained with MouseAnti-Human CD157 Monoclonal Antibody (Catalog # MAB47361,filled histogram) or isotype control antibody (Catalog # MAB002,open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG SecondaryAntibody (Catalog # F0102B) at10 μL/106 cells.
Mu
Species Glossary
Reactivity: Mu
Goat Polyclonal
Host: Goat Polyclonal
Phycoerythrin  
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IC003A

Mouse IgG2a Isotype Control (20102) [Allophycocyanin]

(15 Publications)
200 Tests
Mu
Species Glossary
Reactivity: Mu
Ctrl
Applications: Ctrl
Mouse Monoclonal
Host: Mouse Monoclonal
Allophycocyanin  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 405 IC003V
Alexa Fluor 488 IC003G
Alexa Fluor 594 IC003T
Alexa Fluor 647 IC003R
Alexa Fluor 700 IC003N
Alexa Fluor 750 IC003S
Biotin IC003B
Fluorescein IC003F
PerCP (Peridinin-chlorophyll Protein Complex) IC003C
Phycoerythrin IC003P
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free IC003B
Carrier Free IC003C
Carrier Free IC003F
Carrier Free IC003G
Carrier Free IC003N
Carrier Free IC003P
Carrier Free MAB003
Carrier Free IC003S
Carrier Free IC003T
Carrier Free IC003R
Carrier Free IC003V
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MAB25382

DDR1/DDR2 [p Tyr796, p Tyr740, p Tyr740] Antibody (1119D) [Unconjugated]

(13 Publications)
100 μg
MAB25382
Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human DDR2 untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) for 10 minutes. PVDF membrane was probed with 0.1 μg/mL of Rabbit Anti-Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Monoclonal Antibody (Catalog # MAB25382) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-DDR2 (Y740) at approximately 120-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western
Applications: WB, Simple Western
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB25382-SP
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AF892

RSK1/RSK2 [p Ser221, p Ser227] Antibody

(8 Publications)
100 μg
AF892
RSK1/RSK2 was detected in perfusion fixed frozen sections of mouse brain (Purkinje cells in cerebellum) using 15 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF892) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, IHC
Applications: WB, IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF892-SP
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MAB8529

DYKDDDDK Epitope Tag Antibody (1042E) [Unconjugated]

(2 Reviews)
(4 Publications)
50 μg
MAB8529
Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1, N-terminal DYKDDDDK-tagged UCHL5, and N-terminal DYKDDDDK-tagged RNF20. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-DYKDDDDK Epitope Tag Monoclonal Antibody (Catalog # MAB8529) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific bands were detected for DYKDDDDK-tagged proteins at approximately 55 kDa, 40 kDa, 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
All-Multi
Species Glossary
Reactivity: All-Multi
WB, Simple Western, IP, Flow
Applications: WB, Simple Western, IP, Flow
Rabbit Monoclonal
Host: Rabbit Monoclonal
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 IC8529U-100UG
Alexa Fluor 405 IC8529V-100UG
Alexa Fluor 488 IC8529G-025
Alexa Fluor 488 IC8529G-100
Alexa Fluor 594 IC8529T-100UG
Alexa Fluor 647 IC8529R-025
Alexa Fluor 647 IC8529R-100
Alexa Fluor 700 IC8529N-100UG
Alexa Fluor 750 IC8529S-100UG
Biotin BAM8529
HRP HAM85291
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free IC8529G-025
Carrier Free IC8529R-025
Carrier Free HAM85291
Carrier Free IC8529V-100UG
Carrier Free IC8529T-100UG
Carrier Free IC8529U-100UG
Carrier Free IC8529S-100UG
Carrier Free IC8529N-100UG
Carrier Free IC8529R-100
Carrier Free IC8529G-100
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MAB72391

Ezrin Antibody (2086C) [Unconjugated]

(1 Review)
100 μg
MAB72391
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, OVCAR‑3 human ovarian carcinoma cell line, human lung tissue, mouse lung tissue, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat Ezrin Monoclonal Antibody (Catalog # MAB72391) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Ezrin at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, IHC, Flow, ICC/IF
Applications: WB, IHC, Flow, ICC/IF
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB72391U
Alexa Fluor 350 IC72391U-100UG
Alexa Fluor 405 FAB72391V
Alexa Fluor 405 IC72391V-100UG
Alexa Fluor 488 IC72391G-100UG
Alexa Fluor 488 FAB72391G
Alexa Fluor 532 FAB72391X
Alexa Fluor 594 FAB72391T
Alexa Fluor 594 IC72391T-100UG
Alexa Fluor 647 FAB72391R
Alexa Fluor 647 IC72391R-100UG
Alexa Fluor 700 IC72391N-100UG
Alexa Fluor 700 FAB72391N
Alexa Fluor 750 FAB72391S
Alexa Fluor 750 IC72391S-100UG
Allophycocyanin FAB72391A
Biotin FAB72391B
DyLight 350 FAB72391D
DyLight 405 FAB72391E
DyLight 488 FAB72391K
DyLight 550 FAB72391L
DyLight 594 FAB72391M
DyLight 650 FAB72391W
DyLight 680 FAB72391Y
DyLight 755 FAB72391Z
FITC FAB72391F
HRP FAB72391H
Janelia Fluor 549 FAB72391I
Janelia Fluor 646 FAB72391J
PE FAB72391P
PerCP FAB72391C
mFluor Violet 450 SE FAB72391MFV450
mFluor Violet 500 SE FAB72391MFV500
mFluor Violet 610 SE FAB72391MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB72391-SP
Carrier Free IC72391V-100UG
Carrier Free IC72391U-100UG
Carrier Free IC72391T-100UG
Carrier Free IC72391S-100UG
Carrier Free IC72391R-100UG
Carrier Free IC72391N-100UG
Carrier Free IC72391G-100UG
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MAB8935

Smad2/3 [p Ser465, p Ser467] Antibody (1074A) [Unconjugated]

(11 Publications)
100 μg
MAB8935
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human TGF‑ beta 1 (Catalog # 240-B) for 30 minutes. PVDF membrane was probed with 2 μg/mL of Rabbit Anti-Human Phospho-Smad2/3 (S465/S467) Monoclonal Antibody (Catalog # MAB8935) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Smad2/3 (S465/S467) at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Hu
Species Glossary
Reactivity: Hu
WB
Applications: WB
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB8935-SP
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MAB8930

c-jun [p Ser63] Antibody (1018Y) [Unconjugated]

(1 Review)
(3 Publications)
100 μg
MAB8930
Western blot shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2 ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.5 μg/mL of Rabbit Anti-Human Phospho-c‑Jun (S63) Monoclonal Antibody (Catalog # MAB8930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-c‑Jun (S63) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western, ICC/IF
Applications: WB, Simple Western, ICC/IF
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB8930-SP
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MAB83771

HHEX Antibody (2018B) [Unconjugated]

(6 Publications)
100 μg
MAB83771
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, BaF3 mouse pro-B cell line, and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat HHEX Monoclonal Antibody (Catalog # MAB83771) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HHEX at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Flow, ICC/IF
Applications: WB, Flow, ICC/IF
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB83771U-100UG
Alexa Fluor 405 FAB83771V-100UG
Alexa Fluor 488 FAB83771G-100UG
Alexa Fluor 532 FAB83771X
Alexa Fluor 594 FAB83771T-100UG
Alexa Fluor 647 FAB83771R-100UG
Alexa Fluor 700 FAB83771N-100UG
Alexa Fluor 750 FAB83771S-100UG
Allophycocyanin FAB83771A
Allophycocyanin/Cy7 FAB83771APCCY7
Biotin FAB83771B
DyLight 350 FAB83771D
DyLight 405 FAB83771E
DyLight 488 FAB83771K
DyLight 550 FAB83771L
DyLight 594 FAB83771M
DyLight 650 FAB83771W
DyLight 680 FAB83771Y
DyLight 755 FAB83771Z
FITC FAB83771F
HRP FAB83771H
Janelia Fluor 549 FAB83771I
Janelia Fluor 646 FAB83771J
PE FAB83771P
PE/Atto594 FAB83771PEATT594
PE/Cy5.5 FAB83771PECY55
PE/Cy7 FAB83771PECY7
PerCP FAB83771C
mFluor Violet 450 SE FAB83771MFV450
mFluor Violet 500 SE FAB83771MFV500
mFluor Violet 610 SE FAB83771MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB83771-SP
Carrier Free FAB83771V-100UG
Carrier Free FAB83771U-100UG
Carrier Free FAB83771T-100UG
Carrier Free FAB83771S-100UG
Carrier Free FAB83771R-100UG
Carrier Free FAB83771N-100UG
Carrier Free FAB83771G-100UG
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AF8520

PSAP Antibody [Unconjugated]

(1 Review)
(1 Publication)
100 uL
AF8520
Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human PSAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8520) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for PSAP at approximately 60-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western, ICC/IF
Applications: WB, Simple Western, ICC/IF
Rabbit Polyclonal
Host: Rabbit Polyclonal
Unconjugated  
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