Reactivity | Hu, Mu, RtSpecies Glossary |
Applications | WB, IHC, Flow, ICC/IF |
Clone | 2086C |
Clonality | Monoclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Additional Information | Recombinant Monoclonal Antibody. |
Immunogen | E. coli-derived recombinant human Ezrin Lys438-Arg562 Accession # P15311 |
Specificity | Detects human Ezrin in direct ELISAs and detects human, mouse, and rat Ezrin in Western blots. |
Source | N/A |
Isotype | IgG |
Clonality | Monoclonal |
Host | Rabbit |
Purity Statement | Protein A or G purified from cell culture supernatant |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Reviewed Applications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative | Sodium Azide |
Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. |
EZRIN (also Cytovillin, Villin2 and p81) is a founding member of the ERM family, Band 4.1 Superfamily of proteins. Although its predicted MW is 69 kDa, it runs anomalously at 77-82 kDa in SDS-PAGE. ERZIN is expressed by epithelial cells where it serves as a linker between the cell membrane and the actin cytoskeleton. Its presence is particularly strong in microvilli where it helps organize this structure. In addition, ERZIN also organizes microtubules in lymphocytes at or near the immunological synapse by interacting with Glg1. Human EZRIN is 585 amino acids (aa) in length. It contains a band 4.1 homology/FERM domain that binds CD44, ICAM-1, EBP50 and ERM family members (aa 1-295), a central alpha -helical region (aa 296-352), and a C-terminal ERM and actin-binding/FERM C domain (aa 353-586). EZRIN exists as either a monomer, or a homo/heterodimer. EZRIN is not constitutively active, but must be phosphorylated and unfolded to bind to cytoplasmic proteins. Over aa 438-562, human EZRIN shares 96% aa identity with mouse EZRIN.
Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Louise Speed |
WB | Human and Pig | 08/05/2019 |
Summary
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Secondary Antibodies |
Isotype Controls |
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