Are the FLAG Tag antibodies validated in Simple Western?

Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.

What the theoretical molecular weight for FLAG Tag antibodies?

The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.

Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.

If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.

Will FLAG Tag antibodies recognize FLAG at the C-terminal end?

Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.

Do FLAG Tag antibodies comes in lyophilized form?

The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.

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DYKDDDDK Epitope Tag Antibody (1042E) [Alexa Fluor® 700]

Name: DYKDDDDK Epitope Tag Antibody (1042E) [Alexa Fluor&#174; 700]
Brand: Novus Biologicals
SKU: IC8529N
Price: 359 USD

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Product Details

Summary

Applications	Flow
Clone	1042E
Clonality	Monoclonal
Host	Rabbit
Conjugate	Alexa Fluor 700

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 IC8529U-100UG

Alexa Fluor 405 IC8529V-100UG

Alexa Fluor 488 IC8529G-025

Alexa Fluor 488 IC8529G-100

Alexa Fluor 594 IC8529T-100UG

Alexa Fluor 647 IC8529R-025

Alexa Fluor 647 IC8529R-100

Alexa Fluor 750 IC8529S-100UG

Biotin BAM8529

HRP HAM85291

Unconjugated MAB8529

View Available Formulations

Catalog# & Formulation	Size	Price
Standard Formulation MAB8529

Carrier Free IC8529G-025

Carrier Free IC8529R-025

Carrier Free MAB8529

Carrier Free HAM85291

Carrier Free IC8529V-100UG

Carrier Free IC8529T-100UG

Carrier Free IC8529U-100UG

Carrier Free IC8529S-100UG

Carrier Free IC8529R-100

Carrier Free IC8529G-100

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Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC8529U-100UG
Alexa Fluor 405	IC8529V-100UG
Alexa Fluor 488	IC8529G-025
Alexa Fluor 488	IC8529G-100
Alexa Fluor 594	IC8529T-100UG
Alexa Fluor 647	IC8529R-025
Alexa Fluor 647	IC8529R-100
Alexa Fluor 750	IC8529S-100UG
Biotin	BAM8529
HRP	HAM85291
Unconjugated	MAB8529

DYKDDDDK Epitope Tag Antibody (1042E) [Alexa Fluor® 700] Summary

Additional Information	Recombinant Monoclonal Antibody.
Immunogen	DYKDDDDK epitope tag
Specificity	Detects N-terminal and C-terminal DYKDDDDK (FLAG^®) epitope tagged proteins in Western blot.
Isotype	IgG
Clonality	Monoclonal
Host	Rabbit
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Packaging, Storage & Formulations

Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer	Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for DYKDDDDK Epitope Tag Antibody (IC8529N). (Showing 1 - 5 of 5 FAQ).

Are the FLAG Tag antibodies validated in Simple Western?
- Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.
What the theoretical molecular weight for FLAG Tag antibodies?
- The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.
Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.
- If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.
Will FLAG Tag antibodies recognize FLAG at the C-terminal end?
- Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.
Do FLAG Tag antibodies comes in lyophilized form?
- The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.

Additional DYKDDDDK Epitope Tag Products

DYKDDDDK Epitope Tag Antibodies

Blogs on DYKDDDDK Epitope Tag.

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By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto... Read full blog post.

Hoist the DYKDDDDK Epitope Tag
The DYKDDDDK Epitope Tag antibody was raised against a short, artificial sequence that is widely and commonly used as a protein fusion tag. It is pervasively present in a vast amount of scientific studies, and is a very powerful investigative research... Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

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