| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 594 | NB100-544AF594 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73935 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5937-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6654-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14167 | |||
![Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 used at 0.1 ug/ml. Lower panel: Rabbit anti-RPA32 antibody NB100-332. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Western-Blot-NBP1-23017-img0047.jpg "Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 used at 0.1 ug/ml. Lower panel: Rabbit anti-RPA32 antibody NB100-332. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Immunocytochemistry/Immunofluorescence: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and EPE treated cells (right). Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) used at a dilution of 1:1,000 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100. Counterstain: Alexa Fluor (R) 488 phalloidin (green).](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-23017-img0046.jpg)
![Immunohistochemistry-Paraffin: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Section of mouse gut, MOCK treatment (left) and CIP treatment (right).Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunohistochemistry-Paraffin-NBP1-23017-img0050.jpg)
![Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Samples: Whole cell lysate (50 ug for WB in A; 1 mg for IP, 20% of IP loaded in B) from HeLa Cells that had been treated with EPE (+) or mock treated (-). In B, immunoprecipitated RPA32 was either treated with phosphatase (+) or mock treated (-). Antibodies: Phosphorylated RPA32 was detected using rabbit anti-phospho RPA32 Ser4/Ser8 antibody NBP1-23017 lot 2 at 1 ug/ml. Total RPA32 was detected using rabbit anti-RPA32 antibody NB100-332 at 1 ug/ml. RPA32 was immunoprecipitated using NB100-332 at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds for phospho RPA32 Ser4/Ser8 (A and B) and 1 second for total RPA32 (A and B).](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Western-Blot-NBP1-23017-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel shows Westen Blot for total RPA32 using affinity purified rabbit anti-RPA32 antibody A300-244A at 0.1 ug/ml with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Western-Blot-NBP1-23017-img0042.jpg)
![Immunohistochemistry-Paraffin: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Section of human breast carcinoma, MOCK treatment (left) and CIP treatment(right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) (used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunohistochemistry-Paraffin-NBP1-23017-img0049.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with EPE (+) and/or Lambda PP (+) or mock treated (-). Antibodies: RPA32 (S4/S8) was immunoprecipitated using affinity purified rabbit anti-RPA32 antibody NB100-332. For blotting immunoprecipitated Phospho RPA32 (S4/S8), rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 was used at 0.1 ug/ml. Lower Panel: Rabbit anti-RPA antibody NB100-332. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunoprecipitation-NBP1-23017-img0048.jpg)
![Western Blot: RPA2 [p Ser33] Antibody [NB100-544] - Whole cell lysate (50 ug) from HeLa cells treated with 100 uM etoposide for 16 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S33) antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower panel: Rabbit anti-RPS3 antibody.](http://images.novusbio.com/fullsize/RPA2-[p-Ser33]-Antibody-Western-Blot-NB100-544-img0005.jpg "Western Blot: RPA2 [p Ser33] Antibody [NB100-544] - Whole cell lysate (50 ug) from HeLa cells treated with 100 uM etoposide for 16 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S33) antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower panel: Rabbit anti-RPS3 antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RPA2 [p Ser33] Antibody [NB100-544] - Active DNA end processing in late micronuclei compartments. Representative images (Upper panel) and quantification of RPA signal in MN compartments (Lower left) or RPA foci in nuclei (lower right) of post-IR OVCAR-8 cells after indicated treatments. Pro-resection genes (MRE11, BRCA1 and WDR70) were knockdown by specific siRNA. Percentage of positive RPA2-pS33 MN population was calculated after 36 h of IR treatment Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4347-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPA2-[p-Ser33]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-544-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 [p Ser33] Antibody [NB100-544] - L.36 PDAC cells were treated two hours using 5 mM hydroxyurea to induce RPA32 Ser33 phosphorylation. The antibody produces clear nuclear staining of treated cells. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/RPA2-[p-Ser33]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-544-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 [p Ser33] Antibody [NB100-544] - Active DNA end processing in late micronuclei compartments. Detection and quantification of RPA2 phosphorylation (pSerine 33) and ssDNA by specific antibodies in MN compartments of OVCAR-8 cells 36 h after 2-Gy irradiation. Note the nuclear signals have reduced while those in MN were intense. n = 3 biological repeats. Error bars = s.d. **P < 0.01; ***P < 0.005 (t-test). Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4347-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPA2-[p-Ser33]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-544-img0003.jpg)
![Cellular localization of OS-9 and PHD2.(A) A lectin gel-shift assay was conducted to test for glycosylated proteins. Total cell lysates of U2OS cells were incubated in the presence or absence of the endoglycosidases EndoH and PNGaseF for 6 h at 37°C. Digest products were separated on a reducing 10% SDS-PAGE gel which contained concanavalin A co-polymerized in the top layer of the separating gel to retard mobility of glycosylated proteins [56]. Glycosylated OS-9 is indicated as ‘g’, deglycosylated OS-9 as ‘d’. (B) Detection of OS-9 and PHD2 in the nuclear fraction. HEK293 cells with and without transfection of the plasmid pcDNA3-OS-9 were separated into nuclear fraction (N) and postnuclear supernatant (PS), the latter containing cytoplasm and organelles. Western blot analysis included BiP, GAPDH and lamin A as typical marker proteins for the ER, the cytoplasm and the nucleus, respectively. (C) Detection of OS-9 and PHD2 in the cytoplasm. HEK293 cells were co-transfected with pcDNA3-OS-9 and pPHD2-V5. For hypoxia, cells were exposed to 3% O2 for 4 h. Cells were treated with 50 µg/ml digitonin and centrifuged to obtain a cytoplasmic (C) and an organelle fraction (O) and subjected to immunoblotting. (D) Isolation of cellular endomembranes. HEK293 cells were lysed mechanically by several passages through a 30½G needle. The postnuclear supernatant was processed further by ultra-centrifugation to separate the organelles (O) from the cytosol (C). High salt treatment (1 M KCl) of the organelle fraction produced a wash fraction (W) that contained dissociated peripheral membrane proteins. For immunoblot analysis of subcellular fractionations, cell aliquots were normalized for cell number prior to loading (B–D). Representative Western blots are shown for each subfigure. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-544_rabbit-polyclonal-rpa2-p-ser33-antibody-2322024884420.jpg)
![Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Ajuba associates with RPA in HTC75 and IMR90 cells. IP-Western probed with a total anti-RPA32 antibody after immunoprecipitation with a commercial anti-Ajuba antibody (com.) or anti-peptide serum from IMR90 or HTC75 extracts as indicated. Left panel: total lysates (input) for each cell line, and the pre-immune serum (PI) used as a control for the immunoprecipitation. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fgene.2013.00095/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Western-Blot-NBP2-02182-img0021.jpg "Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Ajuba associates with RPA in HTC75 and IMR90 cells. IP-Western probed with a total anti-RPA32 antibody after immunoprecipitation with a commercial anti-Ajuba antibody (com.) or anti-peptide serum from IMR90 or HTC75 extracts as indicated. Left panel: total lysates (input) for each cell line, and the pre-immune serum (PI) used as a control for the immunoprecipitation. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fgene.2013.00095/abstract), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunocytochemistry-Immunofluorescence-NBP2-02182-img0018.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human thyroid tissue using anti-RPA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP2-02182-img0017.jpg)
![Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of Jurkat cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Flow-Cytometry-NBP2-02182-img0002.jpg)
![Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Western-Blot-NBP2-02182-img0003.jpg)
![Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Western-Blot-NBP2-02182-img0019.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP2-02182-img0005.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human colon tissue using anti-RPA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP2-02182-img0011.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human prostate tissue using anti-RPA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP2-02182-img0016.jpg)
![Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of Hela cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Flow-Cytometry-NBP2-02182-img0001.jpg)
![Immunoprecipitation: RPA2 Antibody (OTI9A1) [NBP2-02182] - (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunoprecipitation-NBP2-02182-img0020.jpg)
![Western Blot: RPA2 Antibody [NB100-332] - Detection of human and mouse RPA32 by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA32 antibody NB100-332 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/RPA2-Antibody-Western-Blot-NB100-332-img0041.jpg "Western Blot: RPA2 Antibody [NB100-332] - Detection of human and mouse RPA32 by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA32 antibody NB100-332 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: RPA2 Antibody [NB100-332] - Human breast carcinoma. Antibody: Affinity purified rabbit anti-RPA32 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NB100-332-img0040.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RPA2 Antibody [NB100-332] - Detection of human RPA32 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-RPA32 antibody NB100-332 used for IP at 3 ug per reaction. RPA32 was also immunoprecipitated by rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal antibody [BL-165-5F1] (NB100-23017). For blotting immunoprecipitated Phospho RPA32 (S4/S8), NB100-23017 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunoprecipitation-NB100-332-img0042.jpg)
![Western Blot: RPA2 Antibody [NBP1-89524] - Analysis using Anti-RPA2 antibody NBP1-89524 (A) shows similar pattern to independent antibody NBP1-89523 (B).](http://images.novusbio.com/fullsize/RPA2-Antibody-Western-Blot-NBP1-89524-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: RPA2 Antibody [NBP1-89524] - Analysis using Anti-RPA2 antibody NBP1-89524 (A) shows similar pattern to independent antibody NBP1-89523 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: RPA2 Antibody [NBP1-89524] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89524-img0007.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0014.jpg)
![Western Blot: RPA2 Antibody [NBP1-89524] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/RPA2-Antibody-Western-Blot-NBP1-89524-img0006.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0011.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Liver shows very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0012.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Testis cancer, carcinoma shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0013.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Tonsil shows moderate nuclear positivity in non-germinal and germinal center cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89524-img0015.jpg)
![Western Blot: RPA2 Antibody (MA34) [NB100-74526] - Analysis of purified RPA protein](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Western-Blot-NB100-74526-img0005.jpg "Western Blot: RPA2 Antibody (MA34) [NB100-74526] - Analysis of purified RPA protein")
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (MA34) [NB100-74526] - Analysis of RPA34 in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunocytochemistry-Immunofluorescence-NB100-74526-img0013.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (MA34) [NB100-74526] - Normal biopsies of deparaffinized Human kidney tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunohistochemistry-Paraffin-NB100-74526-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (MA34) [NB100-74526] - Analysis of RPA34 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunocytochemistry-Immunofluorescence-NB100-74526-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (MA34) [NB100-74526] - Analysis of RPA34 in C6 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunocytochemistry-Immunofluorescence-NB100-74526-img0012.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (MA34) [NB100-74526] - Cancer biopsies of deparaffinized Human breast carcinoma tissue.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunohistochemistry-Paraffin-NB100-74526-img0010.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (MA34) [NB100-74526] - Normal biopsies of deparaffinized Human tonsil tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunohistochemistry-Paraffin-NB100-74526-img0014.jpg)
![Western Blot: RPA2 Antibody (12F3.3) [NB100-158] - HeLa (IR treated) whole cell extract. No radiation, 1hr, 2hr, and 4hr exposure corresponding to lanes 1 through 4 respectively.](http://images.novusbio.com/fullsize/RPA2-Antibody-12F3-3-Western-Blot-NB100-158-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: RPA2 Antibody (12F3.3) [NB100-158] - HeLa (IR treated) whole cell extract. No radiation, 1hr, 2hr, and 4hr exposure corresponding to lanes 1 through 4 respectively.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RPA2 Antibody (12F3.3) [NB100-158] - 2mM hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RPA32 protein stained by RPA32 antibody [12F3.3] Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/RPA2-Antibody-12F3-3-Immunocytochemistry-Immunofluorescence-NB100-158-img0003.jpg)
![Western Blot: RPA2 Antibody (12F3.3) [NB100-158] - Various whole cell extracts (30 ug) were separated by 12 % SDS-PAGE, and blotted with RPA32 antibody diluted by 1:500](http://images.novusbio.com/fullsize/RPA2-Antibody-12F3-3-Western-Blot-NB100-158-img0004.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (RPA2/4774) [NBP3-13764] - Formalin-fixed, paraffin-embedded human tonsil stained with RPA2 antibody (RPA2/4774).](http://images.novusbio.com/fullsize/RPA2-Antibody-RPA2-4774-Immunohistochemistry-Paraffin-NBP3-13764-img0003.jpg "Immunohistochemistry-Paraffin: RPA2 Antibody (RPA2/4774) [NBP3-13764] - Formalin-fixed, paraffin-embedded human tonsil stained with RPA2 antibody (RPA2/4774).")
![Immunohistochemistry-Paraffin: RPA2 Antibody (RPA2/4774) [NBP3-13764] - Formalin-fixed, paraffin-embedded human colon stained with RPA2 antibody (RPA2/4774).](http://images.novusbio.com/fullsize/RPA2-Antibody-RPA2-4774-Immunohistochemistry-Paraffin-NBP3-13764-img0001.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (RPA2/4774) [NBP3-13764] - Formalin-fixed, paraffin-embedded human colon stained with RPA2 antibody (RPA2/4774).](http://images.novusbio.com/fullsize/RPA2-Antibody-RPA2-4774-Immunohistochemistry-Paraffin-NBP3-13764-img0002.jpg)
![Protein Array: RPA2 Antibody (RPA2/4774) [NBP3-13764] - Analysis of Protein Array containing more than 19,000 full-length human proteins using RPA2 Antibody (RPA2/4774).](http://images.novusbio.com/fullsize/RPA2-Antibody-RPA2-4774-Protein-Array-NBP3-13764-img0005.jpg)
![Western Blot: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Detection of human Phospho RPA32 (S4/S8) by WB of HeLa lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal [BL-165-5F1] (NBP2-76396). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/RPA2-p-Ser8-p-Ser4-Antibody-BL-165-5F1-Western-Blot-NBP2-76396-img0001.jpg "Western Blot: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Detection of human Phospho RPA32 (S4/S8) by WB of HeLa lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal [BL-165-5F1] (NBP2-76396). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Immunocytochemistry/Immunofluorescence: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal antibody [BL-165-5F1] .Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/RPA2-p-Ser8-p-Ser4-Antibody-BL-165-5F1-Immunocytochemistry-Immunofluorescence-NBP2-76396-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Detection of Human Phospho RPA32 (S4/S8) in NBF-fixed HeLa cells treated with EPE (right) or mock treated (left) by ICC-IF. Antibody: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal [BL-165-5F1] (NBP2-76396). Detection: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/RPA2-p-Ser8-p-Ser4-Antibody-BL-165-5F1-Immunocytochemistry-Immunofluorescence-NBP2-76396-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RPA2 [p Ser8, p Ser4] Antibody (BL-165-5F1) [NBP2-76396] - Detection of human Phospho RPA32 (S4/S8) by WB of immunoprecipitates from HeLa lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal [BL-165-5F1] (NBP2-76396) and another rabbit anti-RPA32 antibody.](http://images.novusbio.com/fullsize/RPA2-p-Ser8-p-Ser4-Antibody-BL-165-5F1-Immunoprecipitation-NBP2-76396-img0003.jpg)
![Western Blot: RPA2 Antibody [NBP1-89523] - Analysis using Anti-RPA2 antibody NBP1-89523 (A) shows similar pattern to independent antibody NBP1-89524 (B).](http://images.novusbio.com/fullsize/RPA2-Antibody-Western-Blot-NBP1-89523-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: RPA2 Antibody [NBP1-89523] - Analysis using Anti-RPA2 antibody NBP1-89523 (A) shows similar pattern to independent antibody NBP1-89524 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: RPA2 Antibody [NBP1-89523] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89523-img0006.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89523] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89523-img0007.jpg)