| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33006-0.2mg | |||
| BSA Free | NBP2-33006-0.02mg | |||
| Azide and BSA Free | NBP2-34522-0.2mg | |||
| Azide and BSA Free | NBP2-34522-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22120SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34760-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47698-0.1mg | |||
| IHC-Prediluted | NBP2-48132 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47699-0.1mg | |||
| IHC-Prediluted | NBP2-48157 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37641-0.025ml | |||
![Western Blot: alpha-Smooth Muscle Actin Antibody [NB300-978] - (1ug/ml) staining of Mouse Duodenum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Western-Blot-NB300-978-img0007.jpg "Western Blot: alpha-Smooth Muscle Actin Antibody [NB300-978] - (1ug/ml) staining of Mouse Duodenum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody [NB300-978] - SMA antibody staining on mouse fibroblasts. Primary antibody at 1:1000, incubation at 4C overnight. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-978-img0006.jpg)
![Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody [NB300-978] - Infarcted mouse heart frozen tissue section immunostained with alpha-Smooth Muscle Actin Antibody. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Frozen-NB300-978-img0003.jpg)
![alpha-Smooth Muscle Actin Antibody [NB300-978] - (1ug/ml) staining of HeLa (A) and (2ug/ml) NIH3T3 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-NB300-978-img0008.jpg)
![Knockdown Validated: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody diluted at 1:3000. The bands indicate an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Western-Blot-NBP1-30894-img0021.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody diluted at 1:3000. The bands indicate an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.")
![<b>Genetic Strategies Validation. </b>Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin Antibody diluted at a dilution of 1:3000. The bands indicate an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Western-Blot-NBP1-30894-img0011.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Analysis of alpha Smooth Muscle Actin antibody was published by Chuang HM and colleagues](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Western-Blot-NBP1-30894-img0022.jpg)
![Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Smooth Muscle alpha staining on E11.5 mouse Pharyngeal Mesoderm. Fixed with 4% PFA overnight. Blocked with 1% BSA. Primary antibody at 1:100.Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Frozen-NBP1-30894-img0032.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody at a dilution of 1:1000. The band indicates an observed molecular weight of ~42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Western-Blot-NBP1-30894-img0012.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - 30 ug Rat 2 whole cell lysate/extract 10% SDS-PAGE gel with the dilution of alpha-Smooth Muscle Actin Antibody at 1:1000. The band indicates an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Western-Blot-NBP1-30894-img0017.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0023.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cervix. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0024.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0025.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0026.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Rat stomach. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0027.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Rat cervix. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0028.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Mouse duodenum. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0029.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Rat stomach. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0030.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Mouse duodenum. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0031.jpg)
![Immunohistochemistry: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Differential effects of the 20(R/S)-Rg3 stereoisomers on proliferation of VSMCs within plaques. A, Co-immunofluorescence staining of the aortic root for VSMCs (anti-a-SMA antibody, red) and proliferation marker PCNA (green) and DAPI (blue) Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/10.1111/jcmm.13601) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-NBP2-22120-img0016.jpg "Immunohistochemistry: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Differential effects of the 20(R/S)-Rg3 stereoisomers on proliferation of VSMCs within plaques. A, Co-immunofluorescence staining of the aortic root for VSMCs (anti-a-SMA antibody, red) and proliferation marker PCNA (green) and DAPI (blue) Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/10.1111/jcmm.13601) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis of HepG2 cells using (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunocytochemistry-Immunofluorescence-NBP2-22120-img0008.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis using alpha-Smooth Muscle Actin Antibody (4A4) against Hela (1), A431 (2), Jurkat (3), K562 (4), HEK293 (5), HepG2 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate. The theoretical molecular weight of the antibody is 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Western-Blot-NBP2-22120-img0011.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis of paraffin-embedded human duodenum tissues (left) and human esophagus tissues (right) using alpha-Smooth Muscle Actin Antibody (4A4) with DAB staining.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-Paraffin-NBP2-22120-img0009.jpg)
![Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Representative immunohistochemical alpha-Smooth Muscle Actin staining of mouse aortic sinus. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-Frozen-NBP2-22120-img0013.jpg)
![Immunohistochemistry: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Effects of the 20(R/S)-Rg3 stereoisomers on early atherogenesis in diabetic mice. D, F, Representative immunohistochemical a-SMA staining and quantification of the plaque smooth muscle cell content (n = 3). Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/10.1111/jcmm.13601) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-NBP2-22120-img0017.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis of Hela cells using alpha-Smooth Muscle Actin Antibody (4A4) (green) and negative control (purple).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Flow-Cytometry-NBP2-22120-img0007.jpg)
![Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Representative immunohistochemical alpha-Smooth Muscle Actin staining of mouse aortic sinus. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-Frozen-NBP2-22120-img0015.jpg)
![ELISA: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-ELISA-NBP2-22120-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (SPM332) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Immunocytochemistry-Immunofluorescence-NBP2-32808-img0009.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (SPM332) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Staining of paraffin-embedded Leiomyosarcoma using the Azide and BSA-Free version of alpha-Smooth Muscle Actin (SPM332) Antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Immunohistochemistry-Paraffin-NBP2-32808-img0004.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Flow-Intracellular-NBP2-32808-img0008.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP. Image from the PerCP version of this antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Flow-Intracellular-NBP2-32808-img0006.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of alpha smooth muscle Actin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control:Lane 1: A431 cell lysate Lane 2: Hela cell lysate Lane 3: NIH/3T3 cell lysateLane 4: mouse heart tissue lysate Lane 5: mouse smooth muscle tissue lysate](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Western-Blot-NBP2-67440-img0010.jpg "Western Blot: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of alpha smooth muscle Actin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control:Lane 1: A431 cell lysate Lane 2: Hela cell lysate Lane 3: NIH/3T3 cell lysateLane 4: mouse heart tissue lysate Lane 5: mouse smooth muscle tissue lysate")
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in RH-35 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Immunofluorescence analysis of paraffin-embedded human stomach cancer tissue labeling alpha-smooth muscle Actin (washed with PBS. And then probed with the primary antibody alpha smooth muscle Actin (red) at 1/1,000 dilution and ALDH2 (green) at 1/100 dilution overnight at 4 , washed with PBS. iFluor(TM) 488 conjugate-Goat anti-Mouse IgG and iFluor(TM) 647 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0012.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of Jurkat cells with alpha-Smooth Muscle Actin Antibody (SY02-64) at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Flow-Cytometry-NBP2-67440-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in A431 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in A549 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in HepG2 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human gastric carcinoma tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0005.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human liver tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0006.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human tonsil tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0007.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Immunofluorescence analysis of paraffin-embedded human Kidney tissue labeling alpha smooth muscle Actin (washed with PBS. And then probed with the primary antibody alpha smooth muscle Actin (red) at 1/1,000 dilution overnight at 4 , washed with PBS. iFluor(TM) 647 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Immunofluorescence Analysis of HeLa cells labeled with alpha-Smooth Muscle Actin Antibody (ACTA/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunocytochemistry-Immunofluorescence-NBP2-44463-img0008.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Immunofluorescence Analysis of HeLa cells labeled with alpha-Smooth Muscle Actin Antibody (ACTA/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Rat Lung stained with alpha-Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44463-img0004.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (ACTA2/791) NBP2-47698PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Flow-Intracellular-NBP2-44463-img0007.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Human Angiosarcoma stained with alpha-Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44463-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Human Colon Carcinoma stained with alpha-Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44463-img0002.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Human Leiomyosarcoma stained with alpha-Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44463-img0003.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - An intracellular stain was performed on HepG2 cells with alpha-Smooth Muscle Actin Antibody (ACTA2/791) NBP2-47698PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Flow-Cytometry-NBP2-44463-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (1A4 + ACTA2/791) [NBP2-44464] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (1A4 + ACTA2/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-1A4-+-ACTA2-791-Immunocytochemistry-Immunofluorescence-NBP2-44464-img0011.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (1A4 + ACTA2/791) [NBP2-44464] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (1A4 + ACTA2/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4 + ACTA2/791) [NBP2-44464] - Paraffin-embedded Rat Stomach stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-1A4-+-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44464-img0010.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4 + ACTA2/791) [NBP2-44464] - Paraffin-embedded Human Angiosarcoma stained with alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-1A4-+-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44464-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4 + ACTA2/791) [NBP2-44464] - Paraffin-embedded Human Leiomyosarcoma stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-1A4-+-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44464-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4 + ACTA2/791) [NBP2-44464] - Paraffin-embedded Rat Lung stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-1A4-+-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44464-img0009.jpg)
![Immunocytochemistry: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] - Detection of human alpha-Smooth Muscle Actin in FFPE IMR-90 cells by ICC. Antibody: Rabbit anti-alpha Smooth Muscle Actin recombinant monoclonal [BLR082G] (NBP2-78836). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-BLR082G-Immunocytochemistry-NBP2-78836-img0002.jpg "Immunocytochemistry: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] - Detection of human alpha-Smooth Muscle Actin in FFPE IMR-90 cells by ICC. Antibody: Rabbit anti-alpha Smooth Muscle Actin recombinant monoclonal [BLR082G] (NBP2-78836). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] - Detection of human alpha-Smooth Muscle Actin in FFPE breast by IHC. Antibody: Rabbit anti-alpha Smooth Muscle Actin recombinant monoclonal [BLR082G] (NBP2-78836). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-BLR082G-Immunohistochemistry-NBP2-78836-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] - Detection of mouse alpha-Smooth Muscle Actin in FFPE colon carcinoma CT26 by IHC. Antibody: Rabbit anti-alpha Smooth Muscle Actin recombinant monoclonal [BLR082G] (NBP2-78836). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-BLR082G-Immunohistochemistry-NBP2-78836-img0003.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] -: Whole cell lysate (50 ug) fromJurkat, HeLa, IMR-90, HEK293T, and Hep-G2 cells preparedusing NETN lysis buffer. Antibody: Rabbit anti-alphaSmooth Muscle Actin recombinant monoclonal antibody[BLR082G] used at 1:1000. Secondary:HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp2-78836_rabbit-monoclonal-alpha-smooth-muscle-actin-antibody-blr082g-western-blot-213202412531..jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] -Whole cell lysate (10 ug) from CT26, NIH 3T3, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti alpha Smooth Muscle Actin recombinant monoclonal antibody [BLR082G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].](http://images.novusbio.com/fullsize/nbp2-78836_rabbit-monoclonal-alpha-smooth-muscle-actin-antibody-blr082g-western-blot-2132024125440..jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] -: Rabbit anti-alpha SmoothMuscle Actin recombinant monoclonal antibody [BLR082G]. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/nbp2-78836_rabbit-monoclonal-alpha-smooth-muscle-actin-antibody-blr082g-immunohistochemistry-paraffin-213202412568..jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (4A8-2H3) [H00000059-M02] - Western blot analysis of alpha-Smooth Muscle Actin expression in human omentum, serous carcinoma, blotted with alpha-Smooth Muscle Actin Antibody (4A8-2H3). The band indicates an observed molecular weight of 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A8-2H3-Western-Blot-H00000059-M02-img0010.jpg "Western Blot: alpha-Smooth Muscle Actin Antibody (4A8-2H3) [H00000059-M02] - Western blot analysis of alpha-Smooth Muscle Actin expression in human omentum, serous carcinoma, blotted with alpha-Smooth Muscle Actin Antibody (4A8-2H3). The band indicates an observed molecular weight of 42 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (4A8-2H3) [H00000059-M02] - Immunofluorescence analysis of alpha-Smooth Muscle Actin on HeLa cell. Antibody concentration 1 - 10 ug/ml.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A8-2H3-Immunocytochemistry-Immunofluorescence-H00000059-M02-img0008.jpg)
![ELISA: alpha-Smooth Muscle Actin Antibody (4A8-2H3) [H00000059-M02] - Detection limit for recombinant GST tagged alpha-Smooth Muscle Actin Antibody is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A8-2H3-ELISA-H00000059-M02-img0011.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Analysis using alpha-Smooth Muscle Actin Antibody (1H8) against Hela (1), Jurkat (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 (9) cell lysate. Bands indicate an observed molecular weight of ~42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-1H8-Western-Blot-NBP2-37641-img0002.jpg "Western Blot: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Analysis using alpha-Smooth Muscle Actin Antibody (1H8) against Hela (1), Jurkat (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 (9) cell lysate. Bands indicate an observed molecular weight of ~42 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Analysis of NIH/3T3 cells using ACTA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-1H8-Immunocytochemistry-Immunofluorescence-NBP2-37641-img0003.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Analysis of Hela cells using alpha-Smooth Muscle Actin Antibody (1H8) (green) and negative control (purple).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-1H8-Flow-Cytometry-NBP2-37641-img0004.jpg)
![ELISA: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-1H8-ELISA-NBP2-37641-img0001.jpg)
![ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Standard Curve. Image from verified customer review.](http://images.novusbio.com/fullsize/Mouse-alpha-Smooth-Muscle-Actin-ELISA-Kit-Colorimetric-ELISA-NBP2-66429-img0002.jpg "ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Standard Curve. Image from verified customer review.")
![ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - DRC image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-66429_mouse-alpha-smooth-muscle-actin-elisa-kit-colorimetric-elisa-166202313361..jpg)
![ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Standard Curve. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-66429_mouse-alpha-smooth-muscle-actin-elisa-kit-colorimetric-elisa-137202316216.jpg)
![ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Samples were spiked with high concentrations of Mouse alpha-Smooth Muscle Actin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66429_mouse-alpha-smooth-muscle-actin-elisa-kit-colorimetric-132202416225244.jpg)
![ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66429_mouse-alpha-smooth-muscle-actin-elisa-kit-colorimetric-4320249133249.jpg)