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29 Results for "novus"
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930-ADB

Recombinant Human TACE/ADAM17 Protein, CF

(29 Publications)
10 μg
Hu
Species Glossary
Reactivity: Hu
Enzyme Activity
Applications: Enzyme Activity
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MAB9301

TACE/ADAM17 Antibody (111633) [Unconjugated]

(1 Review)
(17 Publications)
100 μg
MAB9301
HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Ectodomain Monoclonal Antibody (Catalog # MAB9301, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Hu
Species Glossary
Reactivity: Hu
WB, Flow, IP, CyTOF-ready, KO
Applications: WB, Flow, IP, CyTOF-ready, KO
23038
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
         
Validated by:          
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB9301U-100UG
Alexa Fluor 405 FAB9301V-100UG
Alexa Fluor 488 FAB9301G-100UG
Alexa Fluor 532 FAB9301X
Alexa Fluor 594 FAB9301T-100UG
Alexa Fluor 647 FAB9301R-100UG
Alexa Fluor 700 FAB9301N-100UG
Alexa Fluor 750 FAB9301S-100UG
Allophycocyanin FAB9301A
Allophycocyanin/Cy7 FAB9301APCCY7
Biotin FAB9301B
DyLight 350 FAB9301D
DyLight 405 FAB9301E
DyLight 488 FAB9301K
DyLight 550 FAB9301L
DyLight 594 FAB9301M
DyLight 650 FAB9301W
DyLight 680 FAB9301Y
DyLight 755 FAB9301Z
HRP FAB9301H
Janelia Fluor 549 FAB9301I
Janelia Fluor 646 FAB9301J
PE/Atto594 FAB9301PEATT594
PE/Cy5.5 FAB9301PECY55
PE/Cy7 FAB9301PECY7
PerCP FAB9301C
Phycoerythrin FAB9301P
mFluor Violet 450 SE FAB9301MFV450
mFluor Violet 500 SE FAB9301MFV500
mFluor Violet 610 SE FAB9301MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free FAB9301P
Carrier Free MAB9301-SP
Carrier Free MAB9301-500
Carrier Free FAB9301V-100UG
Carrier Free FAB9301U-100UG
Carrier Free FAB9301T-100UG
Carrier Free FAB9301S-100UG
Carrier Free FAB9301R-100UG
Carrier Free FAB9301N-100UG
Carrier Free FAB9301G-100UG
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DY930

Human TACE/ADAM17 DuoSet ELISA, 15 Plate

(9 Publications)
1 Kit (for 15 Plates)
DY930
N/A TACE/ADAM17 [Biotin]
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
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NBP1-77044

TACE/ADAM17 Antibody - BSA Free

(1 Review)
(2 Publications)
0.1 mg
NBP1-77044
Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysates or TACE KO celllysates. Antibodies: TACE/ADAM17 NBP1-77044 (0.25 ug/mL) and beta-actin NBP1-76692 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Hu, Rt, Mu
Species Glossary
Reactivity: Hu, Rt, Mu
WB, ELISA, ICC/IF
Applications: WB, ELISA, ICC/IF
Rabbit Polyclonal
Host: Rabbit Polyclonal
IMG-176
Alt Catalog# IMG-176
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
         
Validated by:          
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 NBP1-77044AF350
Alexa Fluor 405 NBP1-77044AF405
Alexa Fluor 488 NBP1-77044AF488
Alexa Fluor 532 NBP1-77044AF532
Alexa Fluor 594 NBP1-77044AF594
Alexa Fluor 647 NBP1-77044AF647
Alexa Fluor 700 NBP1-77044AF700
Alexa Fluor 750 NBP1-77044AF750
Biotin NBP1-77044B
DyLight 350 NBP1-77044UV
DyLight 405 NBP1-77044V
DyLight 488 NBP1-77044G
DyLight 550 NBP1-77044R
DyLight 594 NBP1-77044DL594
DyLight 650 NBP1-77044C
DyLight 680 NBP1-77044FR
DyLight 755 NBP1-77044IR
FITC NBP1-77044F
HRP NBP1-77044H
Janelia Fluor 549 NBP1-77044JF549
Janelia Fluor 646 NBP1-77044JF646
PerCP NBP1-77044PCP
mFluor Violet 450 SE NBP1-77044MFV450
mFluor Violet 500 SE NBP1-77044MFV500
mFluor Violet 610 SE NBP1-77044MFV610
Available Formulations
Formulation Catalog # Availability Price  
BSA Free NBP1-77044-0.025mg
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AF2129

TACE/ADAM17 Antibody [Unconjugated]

(1 Publication)
100 μg
AF2129
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TACE/ADAM17 Cytosolic Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2129) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TACE/ADAM17 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western, IHC, IP, KO
Applications: WB, Simple Western, IHC, IP, KO
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
         
Validated by:          
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF2129-SP
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AF9301

TACE/ADAM17 Antibody

(4 Publications)
100 μg
AF9301
Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TACE/ADAM17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9301) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TACE/ADAM17 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, IHC
Applications: WB, IHC
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF9301-SP
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MAB21291

TACE/ADAM17 Antibody (136133) [Unconjugated]

(1 Publication)
500 μg
MAB21291
Western blot shows lysates of A549 human lung carcinoma cell line and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TACE/ADAM17 Cytosolic Domain Monoclonal Antibody (Catalog # MAB21291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TACE/ADAM17 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, IP, CyTOF-ready, Flow, KO
Applications: WB, IP, CyTOF-ready, Flow, KO
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
         
Validated by:          
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB21291U-100UG
Alexa Fluor 405 FAB21291V-100UG
Alexa Fluor 488 FAB21291G-100UG
Alexa Fluor 532 FAB21291X
Alexa Fluor 594 FAB21291T-100UG
Alexa Fluor 647 FAB21291R-100UG
Alexa Fluor 700 FAB21291N-100UG
Alexa Fluor 750 FAB21291S-100UG
Allophycocyanin FAB21291A
Biotin FAB21291B
DyLight 350 FAB21291D
DyLight 405 FAB21291E
DyLight 488 FAB21291K
DyLight 550 FAB21291L
DyLight 594 FAB21291M
DyLight 650 FAB21291W
DyLight 680 FAB21291Y
DyLight 755 FAB21291Z
FITC FAB21291F
HRP FAB21291H
Janelia Fluor 549 FAB21291I
Janelia Fluor 646 FAB21291J
PE FAB21291P
PerCP FAB21291C
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB21291-SP
Carrier Free FAB21291V-100UG
Carrier Free FAB21291U-100UG
Carrier Free FAB21291T-100UG
Carrier Free FAB21291S-100UG
Carrier Free FAB21291R-100UG
Carrier Free FAB21291N-100UG
Carrier Free FAB21291G-100UG
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NBP2-67179

TACE/ADAM17 Antibody (JM10-35)

(2 Publications)
100 ul
NBP2-67179
Western Blot: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Analysis of ADAM17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW480 cell lysateLane 2: HepG2 cell lysate
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Flow, ICC/IF, IHC, IP
Applications: WB, Flow, ICC/IF, IHC, IP
Rabbit Monoclonal
Host: Rabbit Monoclonal
Unconjugated  
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NBP2-15281

TACE/ADAM17 Antibody

(2 Publications)
0.1 ml
NBP2-15281
Knockout Validated: TACE/ADAM17 Antibody [NBP2-15281] - Wild-type (WT) and ADAM17 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Hu, Mu, Rt, Po, Ca
Species Glossary
Reactivity: Hu, Mu, Rt, Po, Ca
WB, IHC, KO
Applications: WB, IHC, KO
Rabbit Polyclonal
Host: Rabbit Polyclonal
Unconjugated  
         
Validated by:          
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AF930

TACE/ADAM17 Antibody [Unconjugated]

(4 Publications)
100 μg
Hu
Species Glossary
Reactivity: Hu
WB, IHC
Applications: WB, IHC
Chicken Polyclonal
Host: Chicken Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF930
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF930-SP
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MAB9302

TACE/ADAM17 Antibody (111623)

(3 Publications)
500 μg
MAB9302
Effects of the engineered S197P mutation on CD16a shedding in NK cells.NK92 cells transduced with empty vector (vector only), CD16a, or CD16a/S197P were treated without (Unstim.) or with PMA (100ng/ml) for 30 minutes at 37°C (A), with IL-12 and IL-18 (100ng/ml and 400ng/ml, respectively) for 24 hours at 37°C (B), or with Raji cells and rituximab for 60 min at 37°C (C). Cell surface levels of CD16a were determined by flow cytometry. Isotype-matched negative control antibody staining is indicated by a dotted line. (D) Parent NK92 cells and transduced cells expressing CD16a or CD16a/S197P were treated with Raji cells and rituximab in the presence or absence of the ADAM17 inhibitor BMS566394 (5μM) for 60 min at 37°C. Soluble CD16a levels were determined by ELISA. Each treatment condition was repeated 3 times and the data are representative of 3 independent experiments. Bar graphs show mean ± SD. Statistical significance is indicated as ***P<0.001. (E) NK92 cells expressing CD16a or CD16a/S197P were stained with the anti-ADAM17 mAbs M220, 623, 633, or an isotype-matched negative control antibody, as indicated. (F) CD56+CD45+ NK cells derived from mock-transduced iPSCs (left panel) or iPSCs expressing recombinant CD16a or CD16a/S197P (right panels) were incubated with or without K562 target cells for 4 hours at 37°C. For all histogram plots, the x-axis = Log 10 fluorescence, the y-axis = cell number, and the data are representative of at least 3 independent experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0121788), licensed under a CC-BY license. Not internally tested by R&D Systems.
Hu
Species Glossary
Reactivity: Hu
WB, IP
Applications: WB, IP
23038
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB9302-SP
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2978-AD

Recombinant Mouse TACE/ADAM17 Protein, CF

(5 Publications)
10 μg
Mu
Species Glossary
Reactivity: Mu
Enzyme Activity
Applications: Enzyme Activity
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