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22 Results for "novus"
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NBP1-30052

Choline Acetyltransferase/ChAT Antibody

(22 Publications)
0.1 ml
NBP1-30052
Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - The decrease in protein expressions of c-kit, Choline Acetyltransferase/ChAT, nNOS and Cx43 in the colon of elderly humans. Expressions of c-kit, ChAT, nNOS and Cx43 proteins in colonic muscle layers detached from youth and older adults were examined by western blotting (n=4 per group), and similar reduction was observed. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30530917/) licensed under a CC-BY license.
Hu, Mu, Rt, Ch, Gp, Pm
Species Glossary
Reactivity: Hu, Mu, Rt, Ch, Gp, Pm
WB, ICC/IF, IHC, IHC
Applications: WB, ICC/IF, IHC, IHC
Goat Polyclonal
Host: Goat Polyclonal
Unconjugated  
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NBP2-46620

Choline Acetyltransferase/ChAT Antibody (CL3173)

(5 Publications)
0.1 ml
NBP2-46620
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining in human placenta and liver tissues. Corresponding CHAT RNA-seq data are presented for the same tissues.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
ICC/IF, IHC
Applications: ICC/IF, IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
         
Validated by:          
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NB110-89724

Choline Acetyltransferase/ChAT Antibody

(3 Publications)
0.1 ml
NB110-89724
Western Blot: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - Effect of denaturing temperature on Western blot on brain lysates using Rabbit antibody to ChAT.
Mu, Rt
Species Glossary
Reactivity: Mu, Rt
WB, ICC/IF, IHC, IHC
Applications: WB, ICC/IF, IHC, IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
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AF3447

Choline Acetyltransferase/ChAT Antibody [Unconjugated]

(4 Publications)
100 μg
AF3447
Choline Acetyltransferase/ChAT was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 15 µg/mL Goat Anti-Human Choline Acetyltransferase/ChAT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3447) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Hu
Species Glossary
Reactivity: Hu
WB, IHC
Applications: WB, IHC
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF3447
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF3447-SP
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MAB3447

Choline Acetyltransferase/ChAT Antibody (334008)

(4 Publications)
100 μg
MAB3447
Detection and characterization of extracellular ChAT protein in human plasma and CSF.(a) Dot-blot analysis of nine different pooled CSF samples using two different antibodies. (b) The relative amount of ChAT in plasma or CSF, compared to brain homogenates (BH) of AD or control. At 16-fold dilutions, the immunosignals are much stronger in plasma and CSF compared to BH. This demonstrates the relative abundance of the ChAT protein in extracellular fluids. (c) Western-blot characterization of molecular form of ChAT in plasma, CSF and BH. The major detected protein band (*) in the BHs corresponds to a ChAT protein with a Mw of ∼65 kDa. In addition, there are several detected heavier molecular forms of ChAT in the CSF. All samples were loaded on one gel. Note also that the amount of the ∼65 kDa ChAT was so high in plasma that it distorted the gel downward. (d–g) Combinatorial sandwich ELISA results for identification of extracellular ChAT using three different antibody pairs for recombinant human ChAT (rhChAT), and a pooled plasma sample (g) calibrated for ChAT protein concentration using the rhChAT and ELISA setup in (f). (h) Further characterization of the molecular forms of CSF ChAT in consecutive fractions of pooled AD CSF samples separated by sucrose-density gradient technique, and the subsequent quantification of ChAT by sandwich ELISA. The graph represents the average of nine different pooled CSF. This independent analysis provides an identical pattern of molecular forms detected by Western analysis. Due to lack of prior reports, we used analogous terminology, which is used for the counteracting cholinergic enzymes, AChE and BuChE, that is, Gn, where n denotes the number of globular subunits in each detected molecular form of ChAT in CSF. The molecular weights are calculated based on the known Mw of two internal standard proteins. In all dot-blot analyses, 2 µL of each sample (neat or diluted) was used. In the Western blot analysis, each lane was loaded with 15 µL of a mixture, containing 10 µL of sample (neat or diluted) and 5 µL of a 6x concentrated reducing Laemmli loading buffer. All ChAT protein quantifications were done with the ELISA antibody pair’s combination in (g). Anti-ChAT antibodies: RP = rabbit polyclonal antibody (Ab), BGP = biotin-labeled goat polyclonal Ab, MAB = mouse monoclonal Ab. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0065936), licensed under a CC-BY license. Not internally tested by R&D Systems.
Hu
Species Glossary
Reactivity: Hu
WB
Applications: WB
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB3447-SP
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NBP2-46650

Choline Acetyltransferase/ChAT Antibody (CL3169)

0.1 ml
NBP2-46650
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3169) [NBP2-46650] - Choline Acetyltransferase/ChAT Antibody (3169) [NBP2-46650] - Staining in human placenta and liver tissues. Corresponding CHAT RNA-seq data are presented for the same tissues.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
IHC
Applications: IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
         
Validated by:          
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NBP3-26659

Choline Acetyltransferase/ChAT Antibody (8E7)

100 ul
NBP3-26659
Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (8E7) [NBP3-26659] - Image of Choline Acetyltransferase/ChAT Antibody (8E7) diluted at 1:50 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.22% DAB.
Hu, Mu
Species Glossary
Reactivity: Hu, Mu
WB, ELISA, Flow, IHC, ICC/IF
Applications: WB, ELISA, Flow, IHC, ICC/IF
Rabbit Monoclonal
Host: Rabbit Monoclonal
Unconjugated  
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NBP3-15621

Choline Acetyltransferase/ChAT Antibody (1I4V0)

100 ul
NBP3-15621
Western Blot: Choline Acetyltransferase/ChAT Antibody (1I4V0) [NBP3-15621] - Western blot analysis of extracts of SH-SY5Y cells, using Choline Acetyltransferase/ChAT antibody (NBP3-15621) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, ICC/IF, IHC
Applications: WB, ICC/IF, IHC
Rabbit Monoclonal
Host: Rabbit Monoclonal
Unconjugated  
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NBP2-33530

Choline Acetyltransferase/ChAT Antibody

0.1 ml
NBP2-33530
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP2-33530] - Staining of human lymph node shows no positivity in lymphoid cells as expected.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
IHC
Applications: IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
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NB100-98693

Choline Acetyltransferase/ChAT Antibody - Azide and BSA Free

0.1 mg
Hu, Mu, Rt, Pm
Species Glossary
Reactivity: Hu, Mu, Rt, Pm
WB, IHC
Applications: WB, IHC
Sheep Polyclonal
Host: Sheep Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
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NB300-910

Choline Acetyltransferase/ChAT Antibody

(1 Publication)
0.1 mg
NB300-910
Western Blot: Choline Acetyltransferase Antibody [NB300-910] - Analysis of Choline Acetyltransferase in Human Placenta lysate using this antibody at 0.03 ug/ml.
Hu, Po, Bv, Ca
Species Glossary
Reactivity: Hu, Po, Bv, Ca
WB, ELISA
Applications: WB, ELISA
Goat Polyclonal
Host: Goat Polyclonal
Unconjugated  
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H00001103-Q01

Recombinant Human Choline Acetyltransferase/ChAT GST (N-Term) Protein

10 μg
H00001103-Q01
SDS-Page: Recombinant Human Choline Acetyltransferase/ChAT Protein [H00001103-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.
Hu
Species Glossary
Reactivity: Hu
WB, ELISA, MA, AP
Applications: WB, ELISA, MA, AP
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