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4 Results for "novus"

AF360

CCL20/MIP-3 alpha Antibody [Unconjugated]

(23 Publications)

100 μg

AF360

CCL20/MIP-3a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stumulated with LPS using Human CCL20/MIP-3a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CCL20/MIP‑3a was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human CCL20/MIP‑3a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Recombinant Human CCL20/MIP‑3 alpha (Catalog # 360-MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL20/MIP‑3 alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL20/MIP‑3 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360). The ND50 is typically 0.2-1.0 µg/mL.

CCL20/MIP‑3 alpha was detected in immersion fixed paraffin-embedded sections of Human Testis using Goat Anti-Human CCL20/MIP‑3 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to myoid cells, spermatogonia and spermatids.. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF360

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF360-SP

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AF760

CCL20/MIP-3 alpha Antibody [Unconjugated]

(1 Review)

(7 Publications)

100 μg

AF760

Recombinant Mouse CCL20/MIP‑3 alpha (Catalog #760-M3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL20/MIP‑3 alpha (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL20/MIP‑3 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF760). The ND50 is typically 0.5-2.0 µg/mL.

Fatty acid induces Ccl20 expression from dermal blood endothelial cells (BECs) and epidermal keratinocytes. (a) Fold induction of Ccl2, Cxcl16, Ccl20 mRNA in the dermis of ND- or HFD-fed mice in the steady state, as analyzed by qRT-PCR. Results are presented relative to those of ND. The average mRNA expression levels in ND-fed mice are set as 1. (b) Immunohistochemical staining for CCL20 (green) and CD31 (red) in the ear skin of steady state ND and HFD-fed mice. Arrowheads in the panels indicate CCL20+CD31+ cells. Scale bars = 50 μm. (c) Fold induction of Ccl20 mRNA in the steady state epidermis, as analyzed by qRT-PCR. Results are presented relative to those of ND. The average mRNA expression level in ND-fed mice is set as 1. (d) Fold induction of CCL20 mRNA expression in human dermal BECs and epidermal keratinocytes cultured with or without fatty acids for 24 h. Results are presented relative to those of the vehicle-treated group. The average mRNA expression levels in ND-fed mice are set as 1. Pal: palmitic acid, Ste: stearic acid, Ole: oleic acid, Lin: linoleic acid. Results are expressed as the mean ± SEM. p-values were obtained by (a,c) Mann-Whitney-U-test and (d) one way ANOVA. *p ≤ 0.05. Data are pooled from two experiments with four mice (a), two experiments with three mice (c). Data are from one experiment, representative of three independent experiments with three to four mice (b), three experiments performed in triplicate (d). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29074858), licensed under a CC-BY license. Not internally tested by R&D Systems.

High-fat diet-induced obesity exacerbates imiquimod-induced psoriatic dermatitis. (a) Comparison of body weight between normal diet (ND)- and high fat diet (HFD)-fed mice 10 weeks after feeding. (b–d) Imiquimod (IMQ)-induced psoriatic dermatitis in wild-type (WT) mice fed with either a ND- or HFD-diet. Mice were treated with IMQ for five consecutive days. (b) Representative pictures of psoriatic dermatitis in ear skin. (c) Time course of ear swelling response. (d) Epidermal thickness change with or without IMQ treatment for five days. (e) Representative pictures of H&E-stained sections of the ear skin in ND- and HFD-fed mice with or without IMQ treatment for five days. Samples were collected at 24 h after the last IMQ treatment. Scale bars = 50 μm. Results are expressed as the mean ± SD (c) and SEM (a,d). p-values were obtained by Mann-Whitney-U-test. *p ≤ 0.05. Data are from one experiment, representative of six independent experiments with three to four mice (a), three experiments with three to four mice (b–e). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29074858), licensed under a CC-BY license. Not internally tested by R&D Systems.

High-fat diet-induced obesity exacerbates imiquimod-induced psoriatic dermatitis. (a) Comparison of body weight between normal diet (ND)- and high fat diet (HFD)-fed mice 10 weeks after feeding. (b–d) Imiquimod (IMQ)-induced psoriatic dermatitis in wild-type (WT) mice fed with either a ND- or HFD-diet. Mice were treated with IMQ for five consecutive days. (b) Representative pictures of psoriatic dermatitis in ear skin. (c) Time course of ear swelling response. (d) Epidermal thickness change with or without IMQ treatment for five days. (e) Representative pictures of H&E-stained sections of the ear skin in ND- and HFD-fed mice with or without IMQ treatment for five days. Samples were collected at 24 h after the last IMQ treatment. Scale bars = 50 μm. Results are expressed as the mean ± SD (c) and SEM (a,d). p-values were obtained by Mann-Whitney-U-test. *p ≤ 0.05. Data are from one experiment, representative of six independent experiments with three to four mice (a), three experiments with three to four mice (b–e). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29074858), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF760

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF760-SP

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AF540

CCL20/MIP-3 alpha Antibody

(1 Publication)

100 μg

AF540

Recombinant Rat CCL20/MIP‑3 alpha (Catalog # 540-RM) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CCL20/MIP‑3 alpha (5 µg/mL) is neutralized (green line) by increasing concentrations of Rat CCL20/MIP‑3 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF540). The ND50 is typically 30-75 µg/mL.

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF540

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF540-SP

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NBP1-45246

CCL20/MIP-3 alpha Antibody

(1 Publication)

0.1 mg

NBP1-45246

Immunohistochemistry-Paraffin: CCL20/MIP-3 alpha Antibody [NBP1-45246] - (6ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Reactivity:

Hu, Po, Bv

Species Glossary

Reactivity: Hu, Po, Bv

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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