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![Detection of Human and Mouse Histone H2AX [p Ser139] by Western Blot. Samples: Nuclear extract (50 ug) from human HEK293, human melanoma (G361), mouse wildtype embryonic fibroblasts (+/+) or mouse H2AX knockout embryonic fibroblasts (-/-). Antibody: Affinity purified rabbit Histone H2AX [p Ser139] antibody NB100-384 used at 0.1 ug/ml. Detection: Chemiluminescence with 30 second exposure. (NCS, neocarzinostatin - 200 ng/ml, 30 min). Bands appear at an observed molecular weight of ~15 kDa.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Western-Blot-NB100-384-img0012.jpg "<b>Genetic Strategies Validation. </b>Detection of Human and Mouse Histone H2AX [p Ser139] by Western Blot. Samples: Nuclear extract (50 ug) from human HEK293, human melanoma (G361), mouse wildtype embryonic fibroblasts (+/+) or mouse H2AX knockout embryonic fibroblasts (-/-). Antibody: Affinity purified rabbit Histone H2AX [p Ser139] antibody NB100-384 used at 0.1 ug/ml. Detection: Chemiluminescence with 30 second exposure. (NCS, neocarzinostatin - 200 ng/ml, 30 min). Bands appear at an observed molecular weight of ~15 kDa.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Samples: Neocarzinostatin treated asynchronous HeLa cells (left) and untreated asynchronous HeLa cells (right) . Antibody: Affinity purified rabbit Histone H2AX [p Ser139] used at a dilution of 1:5,000 (0.2ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight 594 used at a dilution of 1:100.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-384-img0009.jpg)
![Simple Western lane view shows a specific band for Histone H2AX [p Ser139] in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Simple-Western-NB100-384-img0014.jpg)
![Immunostaining of Histone H2AX [p Ser139] WT1 and 5mC in patients with IgA nephropathy and controls. Examples of PAS staining and immunostaining with Histone H2AX [p Ser139] (green) and WT1 (red), pATM and 5mC in glomeruli of IgA nephropathy and controls. A kidney sample of a 65-year-old male of IgA nephropathy without podocytopathic features. Arrows indicate Histone H2AX [p Ser139] and WT1 double-positive cells. Scale bars: 50um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-57140-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-384-img0022.jpg)
![<b>Biological Strategies Validation. </b>Samples: Nuclear extract from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-). Antibody: Affinity purified rabbit Histone H2AX [p Ser139] antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Band appears at an observed molecular weight of ~17 kDa.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Western-Blot-NB100-384-img0016.jpg)
![<b>Biological Strategies Validation. </b>Detection of human Histone H2AX [p Ser139] by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE for 4 hours (+) or mock treated (-). Antibody: Affinity purified rabbit Histone H2AX [p Ser139] antibody NB100-384 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds. Band appears at an observed molecular weight of ~18 kDa.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Western-Blot-NB100-384-img0019.jpg)
![FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbi Histone H2AX [p Ser139] antibody used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-Paraffin-NB100-384-img0026.jpg)
![Analysis of Histone H2AX [p Ser139] in EPE Treated Jurkat Cells. Cells were treated for 3 hrs in 5ug/ml etoposide, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 0.5 ug anti-KLH or anti-H2AX NB100-384 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- etosposide treated, anti-KLH; Red- untreated, anti-Histone H2AX [p Ser139]; Blue- etoposide treated , anti-Histone H2AX [p Ser139].](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Flow-Cytometry-NB100-384-img0008.jpg)
![FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit Histone H2AX [p Ser139] antibody used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-Paraffin-NB100-384-img0025.jpg)
![Electropherogram image(s) of corresponding Simple Western lane view. Histone H2AX [p Ser139] antibody was used at 5 ug/ml dilution on Jurkat lysate(s).](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Simple-Western-NB100-384-img0015.jpg)
![Immunohistochemistry: Rabbit Polyclonal Histone H2AX [p Ser139] Antibody - Histone H2AX Antibody on mouse cancer tissue. H2AX (Gray) and H2BGFP(Green). Primary antibody dilution: 1:1000 in a 10um slice.](http://images.novusbio.com/fullsize/antibody/nb100-384_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-immunohistochemistry-16102023102936..png)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Biological and molecular analysis of cutaneous wound healing in p73+/+ and p73-/- mice. Representative micrographs of immunohistochemistry (IHC) staining for Histone H2AX [p Ser139] in skin specimens from p73+/+ and p73-/- mice 10 days after wounding. All scale bars represent 50 um. Regions of the skin are labeled as: IFE, HF, epidermal wound edge (WE), and newly-formed epidermis of the wound (W); and the dotted line indicates the border between the WE and W. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0218458), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-2280-img0022.jpg "Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Biological and molecular analysis of cutaneous wound healing in p73+/+ and p73-/- mice. Representative micrographs of immunohistochemistry (IHC) staining for Histone H2AX [p Ser139] in skin specimens from p73+/+ and p73-/- mice 10 days after wounding. All scale bars represent 50 um. Regions of the skin are labeled as: IFE, HF, epidermal wound edge (WE), and newly-formed epidermis of the wound (W); and the dotted line indicates the border between the WE and W. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0218458), licensed under a CC-BY license.")
![Simple Western: Histone H2AX [p Ser139] Antibody [NB100-2280] - Simple Western lane view shows a specific band for Histone H2AX [p Ser139] in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Simple-Western-NB100-2280-img0008.jpg)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Telomere dysfunctional induced foci (TIF) in HBV-related multistep hepatocarcinogenesis and the correlations thereof with stathmin and elongation factor 1alpha (EF1alpha) expression. A. Representative features of colocalization of Histone H2AX [p Ser139] and telomeric DNA in defined lesions of human multistep hepatocarcinogenesis. TIF are indicated by colored arrowheads: blue, DAPI; green, gamma H2AX; red, telomeres; yellow, TIF. Image collected and cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-12-154) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-2280-img0021.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody [NB100-2280] - Detection of human Histone H2AX [p Ser139] antibody by immunohistochemistry. Sample: FFPE section of human seminoma. Antibodies: Affinity purified rabbit Histone H2AX [p Ser139] antibody used at a dilution of 1:500. Detection: DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-Paraffin-NB100-2280-img0023.jpg)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Detection of Mouse Histone H2AX [p Ser139] by Immunohistochemistry. Sample: FFPE section of mouse colon carcinoma CT26. Antibodies: Affinity purified rabbit Histone H2AX [p Ser139] antibody. Detection: DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-2280-img0018.jpg)
![Simple Western: Histone H2AX [p Ser139] Antibody [NB100-2280] - Electropherogram image(s) of corresponding Simple Western lane view. Histone H2AX [p Ser139] antibody was used at 1:20 dilution on Jurkat lysate(s).](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Simple-Western-NB100-2280-img0009.jpg)
![Western Blot: beta-III Tubulin Antibody [NB100-1612] - The levels of glial fibrillary acidic protein (GFAP) and S100beta as astrocytic protein marker proteins in the hippocampal area. Western blot images using GFAP, tubulin beta-3, and beta-actin antibodies to total protein lysate in hippocampal area. The statistical results of internal standardized GFAP/beta-tubulin ratio by beta-actin levels relative to young adult wild-type C57BL/6J. White and black bars indicate the wild-type C57BL/6J and Tet-mev-1 mice, respectively. Data are expressed as mean +/- SD; *P 12 in each group. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12523), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-Western-Blot-NB100-1612-img0006.jpg "Western Blot: beta-III Tubulin Antibody [NB100-1612] - The levels of glial fibrillary acidic protein (GFAP) and S100beta as astrocytic protein marker proteins in the hippocampal area. Western blot images using GFAP, tubulin beta-3, and beta-actin antibodies to total protein lysate in hippocampal area. The statistical results of internal standardized GFAP/beta-tubulin ratio by beta-actin levels relative to young adult wild-type C57BL/6J. White and black bars indicate the wild-type C57BL/6J and Tet-mev-1 mice, respectively. Data are expressed as mean +/- SD; *P 12 in each group. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12523), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody [NB100-1612] - Beta-III Tubulin in rat primary motor neurons. Ab dilution 1:1000 in PBST (0.1% Triton X-100) + 10% GS O/N at 4C. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1612-img0011.jpg)
![Western Blot: beta-III Tubulin Antibody [NB100-1612] - The levels of glial fibrillary acidic protein (GFAP) and S100beta as astrocytic protein marker proteins in the hippocampal area. Western blot images using S100beta, tubulin beta-3, and beta-actin antibodies to total protein lysate in hippocampal area. The statistical results of internal standardized S100beta/beta-tubulin ratio by beta-actin levels relative to young adult wild-type C57BL/6J. White and black bars indicate the wild-type C57BL/6J and Tet-mev-1 mice, respectively. Data are expressed as mean +/- SD; *P 12 in each group. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12523), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-Western-Blot-NB100-1612-img0007.jpg)
![Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - MDA-MB-231 cells were treated with DMSO or Merbarone (100 uM) for 24 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. KAP1 [p Ser824] Antibody (NB100-2350) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Western-Blot-NB100-2350-img0019.jpg "Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - MDA-MB-231 cells were treated with DMSO or Merbarone (100 uM) for 24 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. KAP1 [p Ser824] Antibody (NB100-2350) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody [NB100-2350] - NBF-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with EPE (left) or untreated (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight (R) 594 conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2350-img0015.jpg)
![Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - Human Phospho Kap1 (S824) by Immunohistochemistry. Sample: FFPE serial section of human lung cancer used at a dilution of 1:200 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Immunohistochemistry-Paraffin-NB100-2350-img0014.jpg)
![Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (15 ug) from NIH 3T3 cellstreated with 100 uM etoposide (+) or mock treated (-).Antibodies: Affinity purified rabbit anti-Phospho KAP-1(S824) antibody used forWB at 0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 30 seconds. Lower panel shows westernblot for total KAP-1 using rabbit anti-KAP-1 recombinantmonoclonal antibody at 1:1000.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Western-Blot-NB100-2350-img0017.jpg)
![Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (50 ug) from HEK293Tcells treated with 100 uM etoposide (+) or mock treated(-). Antibodies: Affinity purified rabbit anti-Phospho KAP-1(S824) antibody used forWB at 0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 1 second. Lower panel shows westernblot for total KAP-1 using affinity purified rabbit anti KAP-1 antibody at 0.1 ug/ml.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Western-Blot-NB100-2350-img0018.jpg)
![Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - FFPE serial sections of asynchronous HeLa cells treated with EPE (left) anduntreated HeLa cells (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Immunohistochemistry-Paraffin-NB100-2350-img0013.jpg)
![Immunoprecipitation: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) and rabbit anti-KAP-1 recombinant monoclonal antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated Phospho KAP-1 (S824), Antibody was used at 0.04 ug/ml. To examine total KAP-1, the blot was stripped and then blotted with this antibody at 1:1000 (lower panel). Detection: Chemiluminescence with an exposure time of 1 second.Previous](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Immunoprecipitation-NB100-2350-img0016.jpg)
![Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 used at 0.1 ug/ml. Lower panel: Rabbit anti-RPA32 antibody NB100-332. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Western-Blot-NBP1-23017-img0047.jpg "Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 used at 0.1 ug/ml. Lower panel: Rabbit anti-RPA32 antibody NB100-332. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Immunocytochemistry/Immunofluorescence: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and EPE treated cells (right). Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) used at a dilution of 1:1,000 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100. Counterstain: Alexa Fluor (R) 488 phalloidin (green).](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-23017-img0046.jpg)
![Immunohistochemistry-Paraffin: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Section of mouse gut, MOCK treatment (left) and CIP treatment (right).Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunohistochemistry-Paraffin-NBP1-23017-img0050.jpg)
![Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Samples: Whole cell lysate (50 ug for WB in A; 1 mg for IP, 20% of IP loaded in B) from HeLa Cells that had been treated with EPE (+) or mock treated (-). In B, immunoprecipitated RPA32 was either treated with phosphatase (+) or mock treated (-). Antibodies: Phosphorylated RPA32 was detected using rabbit anti-phospho RPA32 Ser4/Ser8 antibody NBP1-23017 lot 2 at 1 ug/ml. Total RPA32 was detected using rabbit anti-RPA32 antibody NB100-332 at 1 ug/ml. RPA32 was immunoprecipitated using NB100-332 at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds for phospho RPA32 Ser4/Ser8 (A and B) and 1 second for total RPA32 (A and B).](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Western-Blot-NBP1-23017-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel shows Westen Blot for total RPA32 using affinity purified rabbit anti-RPA32 antibody A300-244A at 0.1 ug/ml with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Western-Blot-NBP1-23017-img0042.jpg)
![Immunohistochemistry-Paraffin: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Section of human breast carcinoma, MOCK treatment (left) and CIP treatment(right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) (used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunohistochemistry-Paraffin-NBP1-23017-img0049.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with EPE (+) and/or Lambda PP (+) or mock treated (-). Antibodies: RPA32 (S4/S8) was immunoprecipitated using affinity purified rabbit anti-RPA32 antibody NB100-332. For blotting immunoprecipitated Phospho RPA32 (S4/S8), rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 was used at 0.1 ug/ml. Lower Panel: Rabbit anti-RPA antibody NB100-332. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunoprecipitation-NBP1-23017-img0048.jpg)
![Immunocytochemistry/Immunofluorescence: PKC alpha Antibody (MC5) [NB600-201] - PKC alpha (MC5) antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-MC5-Immunocytochemistry-Immunofluorescence-NB600-201-img0001.jpg "Immunocytochemistry/Immunofluorescence: PKC alpha Antibody (MC5) [NB600-201] - PKC alpha (MC5) antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201] - PKC alpha detected in muse prostate tissue using PKC alpha Antibody (MC5). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-MC5-Immunohistochemistry-Paraffin-NB600-201-img0004.jpg)
![Flow Cytometry: PKC alpha Antibody (MC5) [NB600-201] - Detection of PKC alpha in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- PKC alpha Monoclonal Antibody (Catalog # NB600-201, filled histogram), or Mouse IgG2A isotype control (Catalog # MAB003, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-MC5-Flow-Cytometry-NB600-201-img0003.jpg)
![Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201] - IHC staining of PKC alpha in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-MC5-Immunohistochemistry-Paraffin-NB600-201-img0002.jpg)
![Western Blot: RPA2 [p Ser33] Antibody [NB100-544] - Whole cell lysate (50 ug) from HeLa cells treated with 100 uM etoposide for 16 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S33) antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower panel: Rabbit anti-RPS3 antibody.](http://images.novusbio.com/fullsize/RPA2-[p-Ser33]-Antibody-Western-Blot-NB100-544-img0005.jpg "Western Blot: RPA2 [p Ser33] Antibody [NB100-544] - Whole cell lysate (50 ug) from HeLa cells treated with 100 uM etoposide for 16 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S33) antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower panel: Rabbit anti-RPS3 antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RPA2 [p Ser33] Antibody [NB100-544] - Active DNA end processing in late micronuclei compartments. Representative images (Upper panel) and quantification of RPA signal in MN compartments (Lower left) or RPA foci in nuclei (lower right) of post-IR OVCAR-8 cells after indicated treatments. Pro-resection genes (MRE11, BRCA1 and WDR70) were knockdown by specific siRNA. Percentage of positive RPA2-pS33 MN population was calculated after 36 h of IR treatment Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4347-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPA2-[p-Ser33]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-544-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 [p Ser33] Antibody [NB100-544] - L.36 PDAC cells were treated two hours using 5 mM hydroxyurea to induce RPA32 Ser33 phosphorylation. The antibody produces clear nuclear staining of treated cells. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/RPA2-[p-Ser33]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-544-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 [p Ser33] Antibody [NB100-544] - Active DNA end processing in late micronuclei compartments. Detection and quantification of RPA2 phosphorylation (pSerine 33) and ssDNA by specific antibodies in MN compartments of OVCAR-8 cells 36 h after 2-Gy irradiation. Note the nuclear signals have reduced while those in MN were intense. n = 3 biological repeats. Error bars = s.d. **P < 0.01; ***P < 0.005 (t-test). Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4347-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPA2-[p-Ser33]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-544-img0003.jpg)
![Cellular localization of OS-9 and PHD2.(A) A lectin gel-shift assay was conducted to test for glycosylated proteins. Total cell lysates of U2OS cells were incubated in the presence or absence of the endoglycosidases EndoH and PNGaseF for 6 h at 37°C. Digest products were separated on a reducing 10% SDS-PAGE gel which contained concanavalin A co-polymerized in the top layer of the separating gel to retard mobility of glycosylated proteins [56]. Glycosylated OS-9 is indicated as ‘g’, deglycosylated OS-9 as ‘d’. (B) Detection of OS-9 and PHD2 in the nuclear fraction. HEK293 cells with and without transfection of the plasmid pcDNA3-OS-9 were separated into nuclear fraction (N) and postnuclear supernatant (PS), the latter containing cytoplasm and organelles. Western blot analysis included BiP, GAPDH and lamin A as typical marker proteins for the ER, the cytoplasm and the nucleus, respectively. (C) Detection of OS-9 and PHD2 in the cytoplasm. HEK293 cells were co-transfected with pcDNA3-OS-9 and pPHD2-V5. For hypoxia, cells were exposed to 3% O2 for 4 h. Cells were treated with 50 µg/ml digitonin and centrifuged to obtain a cytoplasmic (C) and an organelle fraction (O) and subjected to immunoblotting. (D) Isolation of cellular endomembranes. HEK293 cells were lysed mechanically by several passages through a 30½G needle. The postnuclear supernatant was processed further by ultra-centrifugation to separate the organelles (O) from the cytosol (C). High salt treatment (1 M KCl) of the organelle fraction produced a wash fraction (W) that contained dissociated peripheral membrane proteins. For immunoblot analysis of subcellular fractionations, cell aliquots were normalized for cell number prior to loading (B–D). Representative Western blots are shown for each subfigure. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-544_rabbit-polyclonal-rpa2-p-ser33-antibody-2322024884420.jpg)
![Simple Western: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Simple Western lane view shows a specific band for Histone H2AX [p Ser139] in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Simple-Western-NB100-74435-img0012.jpg "Simple Western: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Simple Western lane view shows a specific band for Histone H2AX [p Ser139] in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Staining of Phospho-H2AX in A549 cells. Cells were treated with 50 uM EPE for 1 hour (left panel) or with vehicle (right control: 0.1% DMSO in media).](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Staining using NB100-74435, treatment with paraquat and iron induces MnSOD and Phosphorylation of H2AX in RAW 264.7 macrophages. Cells were treated for 20 hours with paraquat (500 uM) and iron (200 ug/ml) and stained with anti-Phospho-H2AX antibody.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Western blot analysis of Jurkat cells (Lane 1) and Jurkat cells treated with staurosporine (Lane 2) blotted with Histone H2AX [p Ser139] antibody (3F2). Bands indicate an observed molecular weight of ~16 kDa.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Western-Blot-NB100-74435-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - STED image of MDA-MB-231 cells after 2 Gy irradiation. Blue channel: DAPI, window level [0 255]. Green channel: secondary antibody Alexa Fluor 488, window level [140 255]. Scale bar: 20 um. Image from verified customer review.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Immunofluorescence analysis using Histone H2AX [p Ser139] antibody (3F2).](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - IF image of phosphorylated H2AX in gamma irradiated Hela cells.](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Immunocytochemistry-Immunofluorescence-NB100-74435-img0015.jpg)
![Simple Western: Histone H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Electropherogram image(s) of corresponding Simple Western lane view. Histone H2AX [p Ser139] antibody (3F2) was used at 10 ug/ml dilution on Jurkat lysate(s).](http://images.novusbio.com/fullsize/gamma-H2AX-p-Ser139-Antibody-3F2-Simple-Western-NB100-74435-img0013.jpg)
![Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis using TGF beta 1 mAb against human LAP (TGF-beta 1) recombinant protein. (Expected MW is 41 kDa)](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Western-Blot-NBP2-22114-img0002.jpg "Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis using TGF beta 1 mAb against human LAP (TGF-beta 1) recombinant protein. (Expected MW is 41 kDa)")
![Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Immunohistochemical analysis of paraffin-embedded lymphoid tissue tissues using LAP (TGF-beta 1) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Immunohistochemistry-Paraffin-NBP2-22114-img0005.jpg)
![Flow Cytometry: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Flow cytometric analysis of A549 cells using LAP (TGF-beta 1) mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Flow-Cytometry-NBP2-22114-img0003.jpg)
![Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis of human stomach tissue (A) and human small intestine tissue (B) using TGF beta 1 antibody (NBP2-22114) at 2 ug/ml.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Western-Blot-NBP2-22114-img0007.jpg)
![Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Human tonsil. Image from verified customer review.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Immunohistochemistry-Paraffin-NBP2-22114-img0006.jpg)
![Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using LAP (TGF-beta 1) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Immunohistochemistry-Paraffin-NBP2-22114-img0004.jpg)
![ELISA: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-ELISA-NBP2-22114-img0001.jpg)
![Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Western blot analysis of MDA-MB-231 cells transfected with/without an IL-8 silencer RNA (siIL-8) to evaluate the expression of MUC-1, VCAM-1 (NBP1-28404), and ICAM-1 (NBP2-22541). GAPDH load control was reused for illustrative purposes. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01767/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Western-Blot-NBP2-22541-img0015.jpg "<b>Biological Strategies Validation. </b>Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Western blot analysis of MDA-MB-231 cells transfected with/without an IL-8 silencer RNA (siIL-8) to evaluate the expression of MUC-1, VCAM-1 (NBP1-28404), and ICAM-1 (NBP2-22541). GAPDH load control was reused for illustrative purposes. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01767/full), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Frozen: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Staining of frozen rat spleen with Mouse anti Rat CD54.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Immunohistochemistry-Frozen-NBP2-22541-img0011.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in C6 cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Flow-Cytometry-NBP2-22541-img0014.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse kidney tissue.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Immunohistochemistry-Paraffin-NBP2-22541-img0001.jpg)
![Immunohistochemistry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse lung tissue.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Immunohistochemistry-NBP2-22541-img0002.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse spleen tissue.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Immunohistochemistry-Paraffin-NBP2-22541-img0003.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Staining of stimulated rat spleen cells with mouse anti-rat CD54 (Alexa488).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Flow-Cytometry-NBP2-22541-img0010.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in Ramos cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Flow-Cytometry-NBP2-22541-img0012.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in Raji cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Flow-Cytometry-NBP2-22541-img0013.jpg)
![Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-R1JHL-Western-Blot-NB300-118-img0001.jpg "Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor.")
![Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - The blots show the examples of immunobands against NMDAR1 and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. Image collected and cropped by CiteAb from the following publication (//www.jbiomedsci.com/content/18/1/64) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-R1JHL-Western-Blot-NB300-118-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - NR1 surface staining of mouse corical neruons. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-R1JHL-Immunocytochemistry-Immunofluorescence-NB300-118-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mouse neuron stained with anti-NMDAR1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-R1JHL-Immunocytochemistry-Immunofluorescence-NB300-118-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mixed rat cortical cultures (21 DIV) were labelled with anti-NMDAR1 (1:200) and anti-mouse CF568 (1:250). The antibody labels small punctae along the neuronal processes. No distinct features such as spines are visible. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-R1JHL-Immunocytochemistry-Immunofluorescence-NB300-118-img0004.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Human Phospho RNA Polymerase II (S2) by Western Blot. Samples: Whole cell lysate (50 and 15 ug) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody NB100-1805 was used for WB at 0.04 ug/ml (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody was used at 1 ug/ml (lower panel). Detection: Chemiluminescence with exposure time of 3 seconds.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Western-Blot-NB100-1805-img0027.jpg "<b>Biological Strategies Validation. </b>Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Human Phospho RNA Polymerase II (S2) by Western Blot. Samples: Whole cell lysate (50 and 15 ug) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody NB100-1805 was used for WB at 0.04 ug/ml (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody was used at 1 ug/ml (lower panel). Detection: Chemiluminescence with exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti-PhosphoRNA Polymerase II (S2) used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Immunohistochemistry-Paraffin-NB100-1805-img0029.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Hela whole cell lysate (50 ug each lane) loaded and probed by anti-RNA polymerase II Antibody. NB100-1805 was pre-incubated without peptide (-), with non-phosphorylated peptide (NP), with phosphorylated S2 peptide, or with phosphorylated S5 peptide.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Western-Blot-NB100-1805-img0021.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Whole cell lysate (15 and 50 ug) from NIH3T3 cells. Antibody used at 0.08 ug/ml.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Western-Blot-NB100-1805-img0022.jpg)
![Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Analysis of POLR2A in MCF-7 xenograft using anti-POLR2A [p Ser2] antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Immunohistochemistry-Paraffin-NB100-1805-img0023.jpg)
![Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Human ovarian carcinoma. Antibody: Affinity purified rabbit antiPhospho-RNA Polymerase II (S2) used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Immunohistochemistry-Paraffin-NB100-1805-img0028.jpg)
![RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Phospho RNA Polymerase II (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1 mg per IP reaction, 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. immunoprecipitates were either phosphatase treated (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody NB100-1805 used for IP at 5 ug per reaction and another Affinity purified rabbit anti-RNA Polymerase II antibody used for IP at 5 ug per reaction. For blotting immunoprecipitated Phospho RNA Polymerase II (S2) and total RNA Polymerase II, NB100-1805 was used at 0.1 ug/ml (upper panel) and the other antibody was used at 1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 10 seconds (upper) and 75 seconds (lower).](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-NB100-1805-img0030.jpg)