Histone H2AX [p Ser139] Antibody (3F2)

Licensed to Novus Biologicals LLC under U.S. Patent Nos. 6,362,317 and 6,884,873.

Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). The H2AX variant represents between 2-25% of total H2A (1-3). Human H2AX protein is 143 amino acids in length and has a theoretical molecular weight of 15 kDa (5). As a result of DSBs, H2AX becomes phosphorylated at serine-139 and gamma H2AX is generated as a response to genomic instability (1-4). Gamma H2AX and its phosphorylation can be detected through western blotting and immunofluorescence, serving as a biomarker for DSB frequency (1-4). Ataxia telangiectasia mutated (ATM) is considered a key protein for H2AX phosphorylation (1-4). Upon the phosphorylation and formation of gamma H2AX, many repair proteins become associated including breast cancer 1 protein (BRCA1), NBS1 (Nijmegen breakage syndrome 1)/hMRE11 (human meiotic recombination 11 protein)/ hRAD50 (N/M/R) repair complex, and 53BP1 (p53 binding protein 1) (1-4). Gamma H2AX detection assays are currently utilized for a variety of purposes, from studying DNA repair to drug development and radiation biodosimetry (4).

References

1. Palla, V. V., Karaolanis, G., Katafigiotis, I., Anastasiou, I., Patapis, P., Dimitroulis, D., & Perrea, D. (2017). gamma-H2AX: Can it be established as a classical cancer prognostic factor?. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. https://doi.org/10.1177/1010428317695931

2. Kuo, L. J., & Yang, L. X. (2008). Gamma-H2AX - a novel biomarker for DNA double-strand breaks. In vivo (Athens, Greece).

3. Kinner, A., Wu, W., Staudt, C., & Iliakis, G. (2008). Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic acids research. https://doi.org/10.1093/nar/gkn550

4. Redon, C. E., Weyemi, U., Parekh, P. R., Huang, D., Burrell, A. S., & Bonner, W. M. (2012). gamma-H2AX and other histone post-translational modifications in the clinic. Biochimica et biophysica acta. https://doi.org/10.1016/j.bbagrm.2012.02.021

5. H2AX: Uniprot (P16104)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 6 applications: ICC/IF, IF/IHC, IHC-P, IM, IP, WB.

Showing Publications 1 - 10 of 19. Show All 19 Publications. Collapse Publications.

Publications using NB100-74435	Applications	Species
Admasu TD, Kim K, Rae M et al. Selective ablation of primary and paracrine senescent cells by targeting iron dyshomeostasis Cell reports 2023-02-06 [PMID: 36753419] (ICC/IF, Human)	ICC/IF	Human
Kim K, Admasu TD, Stolzing A, Sharma A Enhanced co-culture and enrichment of human natural killer cells for the selective clearance of senescent cells Aging 2022-03-04 [PMID: 35245208] (ICC/IF, Human)	ICC/IF	Human
PAramo M, SantamarIa E, Idoate MA et al. A new animal model of atrophy-hypertrophy complex and liver damage following Yttrium-90 lobar selective internal radiation therapy in rabbits Scientific reports 2022-02-02 [PMID: 35110610] (IHC-P, Rabbit)	IHC-P	Rabbit
Sugaya K Characterization of cells expressing MRI reporters for the analysis of epigenetics Analytical biochemistry 2021-09-30 [PMID: 34600867] (IM)	IM
Wang H, Muthu Karuppan M, Devadoss D et al. TFEB protein expression is reduced in aged brains and its overexpression mitigates senescence-associated biomarkers and memory deficits in mice Neurobiology of Aging 2021-10-01 [PMID: 34229273]
Bui TM, Butin-Israeli V, Wiesolek HL et al. Neutrophils Alter DNA Repair Landscape to Impact Survival and Shape Distinct Therapeutic Phenotypes of Colorectal Cancer Gastroenterology 2021-03-19 [PMID: 33753103] (Mouse)		Mouse
Kobayashi, Y, Tata, A Et al. Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis. Nat Cell Biol 2020-08-01 [PMID: 32661339] (IP, Human)	IP	Human
Pelz L, Purfurst B, et al. The cell adhesion molecule BT-IgSF is essential for a functional blood-testis barrier and male fertility in mice. J Biol Chem 2017-12-29 [PMID: 29123028] (IF/IHC, Mouse)	IF/IHC	Mouse
Hatanaka M, Anderson-Baucum E, Lakhter A et al. Chronic high fat feeding restricts islet mRNA translation initiation independently of ER stress via DNA damage and p53 activation Sci Rep. 2017-06-18 [PMID: 28630491] (IHC-P, Human)	IHC-P	Human
Singh M, Widmann T, Cortes J et al. The phylogenetically distinct early human embryo bioRxiv 2018-05-18 (IF/IHC, Human)	IF/IHC	Human
Singh M, Kondrashkina AM, Widmann TJ et al. A new human embryonic cell type associated with activity of young transposable elements allows definition of the inner cell mass PLoS biology 2023-06-01 [PMID: 37339119]
Trier I, Black EM, Joo YK, Kabeche L ATR protects centromere identity by promoting DAXX association with PML nuclear bodies Cell reports 2023-05-30 [PMID: 37163376] (ICC/IF, Human)	ICC/IF	Human
Vigasova K, Durdik M, Jakl L et al. Chemotherapy and cryopreservation affects DNA repair foci in lymphocytes of breast cancer patients International journal of radiation biology 2023-05-18 [PMID: 37145321]
Bhardwaj P, Iyengar NM, Zahid H et al. Obesity promotes breast epithelium DNA damage in women carrying a germline mutation in BRCA1 or BRCA2 Science translational medicine 2023-02-22 [PMID: 36812344]
L�pez-Valverde JA, Jim�nez-Ortega E, Leal A. Clinical Feasibility Study of Gold Nanoparticles as Theragnostic Agents for Precision Radiotherapy Biomedicines 2022-05-23 [PMID: 35625950] (ICC/IF)	ICC/IF
Elsaid MY, Shahi A, Wang AR et al. Enhanced Radiosensitivity in Solid Tumors using a Tumor-Selective Alkyl Phosphocholine Analog Mol. Cancer Ther. 2018-08-14 [PMID: 30108133] (IHC-P, Human)	IHC-P	Human
Hayashi M, Yoshida K, Kitada K et al. Low-dose irradiation of mouse embryos increases Smad-p21 pathway activity and preserves pluripotency J. Assist. Reprod. Genet. 2018-03-16 [PMID: 29546598] (WB, Mouse)	WB	Mouse
Szczepny A, Carey K, McKenzie L et al. The tumor suppressor Hic1 maintains chromosomal stability independent of Tp53 Oncogene 2018-01-25 [PMID: 29367758] (WB, Mouse)	WB	Mouse
Bui TM Dissecting the Diverse Phenotypes and Pathological Impacts of Neutrophils in Colitis-to-CRC Progression Thesis 2022-01-01
Show All 19 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	IF	Human	05/17/2022	View Summary Application Immunofluorescence Sample Tested MDA MB 231 cells Species Human Lot VL317613

Array NB100-74435

• Histone H2AX Antibodies
• Histone H2AX Lysate
• Histone H2AX Proteins
• Histone H2AX ELISA