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![Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Immunostaining of severe acute respiratory syndrome coronavirus 2 in pulmonary tissues from fatal coronavirus disease cases. A) P5 (Patient 5): scattered immunostaining of tracheal epithelial cells. B) P5: higher magnification shows immunostaining of ciliated cells. C) P8: immunostaining of desquamated type I pneumocyte in an alveolar lumen. D) P4: colocalization of SARS-CoV-2 viral antigen (red) with type II pneumocyte stained by surfactant (brown; arrow). E) P4: colocalization of SARS-CoV-2 viral antigen (red) with macrophages stained by CD163 (brown; arrows); virus immunostaining within type II pneumocytes is also seen (arrowheads). F) P4: extensive immunostaining of hyaline membranes in a region of exudative DAD. G) P3: scattered immunostaining within macrophage in hilar lymph node; anthracosis is also present. Emerg Infect Dis. 2020 May 21;26(9) 10.3201/eid2609.202095, PMID: 32437316](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NB100-56576-img0005.jpg "Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Immunostaining of severe acute respiratory syndrome coronavirus 2 in pulmonary tissues from fatal coronavirus disease cases. A) P5 (Patient 5): scattered immunostaining of tracheal epithelial cells. B) P5: higher magnification shows immunostaining of ciliated cells. C) P8: immunostaining of desquamated type I pneumocyte in an alveolar lumen. D) P4: colocalization of SARS-CoV-2 viral antigen (red) with type II pneumocyte stained by surfactant (brown; arrow). E) P4: colocalization of SARS-CoV-2 viral antigen (red) with macrophages stained by CD163 (brown; arrows); virus immunostaining within type II pneumocytes is also seen (arrowheads). F) P4: extensive immunostaining of hyaline membranes in a region of exudative DAD. G) P3: scattered immunostaining within macrophage in hilar lymph node; anthracosis is also present. Emerg Infect Dis. 2020 May 21;26(9) 10.3201/eid2609.202095, PMID: 32437316")
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Pathological changes in rhesus macaques infected with SARS-CoV-2. (g) SARSCoV-2 antigen is detected by immunohistochemistry in type I pneumocytes. Magnification 400x. (j) SARS-CoV-2 antigen is detected by immunohistochemistry in type I pneumocytes (asterisk) and type II pneumocytes (arrow) as well as alveolar macrophages (arrowheads). Magnification 400x. (k) SARS-CoV-2 antigen is detected by immunohistochemistry in mediastinal lymph node. Magnification 400x. (l) SARSCoV-2 antigen is detected by immunohistochemistry in macrophages and lymphocytes in the lamina propria of the cecum. Magnification 400x. bioRxiv March 21, 2020 //doi.org/10.1101/2020.03.21.001628](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NB100-56576-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: SARS Nucleocapsid Protein Antibody [NB100-56576] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue were probed for SARS-CoV-2 viral RNA (ACD anti-sense specific probe v-nCoV2019-S (848561); Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56576] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Dual-RNAscope-ISH-IHC-NB100-56576-img0006.jpg)
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Characteristics of 2B4 cells clonally derived from human bronchial epithelial Calu-3 cells. Finally, 2B4 cells (passage 6) were infected with SARS-CoV (MOI = 0.1) for 24, 48, and 72 hrs before being fixed with 4% paraformaldehyde for monitoring the morphological changes of infected cells, as visualized by the expression of SARS-CoV NP protein (red) by using the standard IHC (F). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0008729) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NB100-56576-img0003.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56576] - Western blot shows recombinant SARS-CoV-2 Nucleocapsid protein. PVDF membrane was probed with 1 ug/mL of Rabbit Anti-SARS-CoV-2 Nucleocapsid Polyclonal Antibody (Catalog # NB100-56576) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for SARS-CoV-2 Nucleocapsid at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56576-img0007.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56576] - analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56576-img0002.jpg)
![Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows recombinant SARS-CoV-2 Nucleocapsid Protein (Catalog # 10474-CV), loaded at 20 ng/mL. A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) using a serial dilution of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Simple-Western-NB100-56683-img0004.jpg "Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows recombinant SARS-CoV-2 Nucleocapsid Protein (Catalog # 10474-CV), loaded at 20 ng/mL. A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) using a serial dilution of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.")
![<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: SARS Nucleocapsid Protein Antibody [NB100-56683] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue were probed for SARS-CoV-2 viral RNA (ACD anti-sense specific probe v-nCoV2019-S [848561]); Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56683] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Dual-RNAscope-ISH-IHC-NB100-56683-img0005.jpg)
![Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows lysates of SARS-CoV-2 (1:50), MERS (1:100), OC43 (1:100), and 229E (1:100). A specific band was detected for SARS-CoV-2 Nucleocapsid Protein at approximately 60 kDa (as indicated) only in the SARS-CoV-2 lysate using 25 ug/mL of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (Catalog # NB100-56683) followed by incubation with HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Note: some reactivity observed with FL Std 230. SARS-CoV-2 lysate courtesy of University of Maryland.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Simple-Western-NB100-56683-img0003.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56683] - Analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody at a 1:2000 dilution.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56683-img0002.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Western blot using Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46 kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h followed by detection using IRDy(TM) 800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:10,000. The fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NBP2-44205-img0004.jpg "Western Blot: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Western blot using Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46 kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h followed by detection using IRDy(TM) 800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:10,000. The fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
![Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein synthesis. VeroE6 cells were infected with rSARS-CoV-2. Mock: non-infected cells. At 48 h.p.i, cells were fixed and prepared for immunofluorescence staining with primary antibodies directed against double-stranded RNA (dsRNA) [green] and SARS-CoV Nucleocapsid (N) [red]. Nuclear Counterstain DAPI [blue].](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44205-img0003.jpg)
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Immunohistochemistry results showing positive staining in placenta tissue using Anti-SARS-CoV Nucleocapsid (N) Antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NBP2-44205-img0005.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Western Blot of Rabbit Anti-SARS CoV Nucleocapsid (N) Protein Antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: SARS CoV Nucleocapsid (N) Protein [20 ng]. Primary Antibody: Anti-SARS CoV Nucleocapsid (N) Protein Antibody at 1.0 ug/mL overnight at 2-8 degrees C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40000 for 30 mins at RT. Predicted MW: ~46 kDa. Observed MW: ~48 kDa.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NBP2-44205-img0001.jpg)
![ELISA: SARS Nucleocapsid Protein Antibody [NBP2-44205] - ELISA results of Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein. Each well was coated in duplicate with 0.5ug/mL of SARS CoV-1 [Red Line], SARS CoV-2 Nc [Green Line], MERS CoV N [Blue Line]. The starting dilution of antibody was 2 ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. SARS CoV-1N IC50: 11ng/mL, SARS CoV-2N IC50: 15 ng/mL. Assay performed using Goat Anti-Rabbit IgG HRP conjugated (p/n 611-1302) and TMB substrate (p/n TMBE-1000).](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-ELISA-NBP2-44205-img0006.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56050] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56050-img0002.jpg "Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56050] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.")
![Western Blot: SARS Nucleocapsid Protein Antibody (05) [NBP3-06413] - Anti-SARS-NP mouse monoclonal antibody at 1:1000 dilution.Lane A: SARS-CoV NP Protein (30ng)Lane B: SARS-CoV NP Protein (5ng)Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (30ng)Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (5ng)Secondary: Goat Anti-Mouse IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-05-Western-Blot-NBP3-06413-img0001.jpg "Western Blot: SARS Nucleocapsid Protein Antibody (05) [NBP3-06413] - Anti-SARS-NP mouse monoclonal antibody at 1:1000 dilution.Lane A: SARS-CoV NP Protein (30ng)Lane B: SARS-CoV NP Protein (5ng)Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (30ng)Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (5ng)Secondary: Goat Anti-Mouse IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.")
![Western Blot: SARS Nucleocapsid Protein Antibody (14B3D) [NBP3-07063] - Western Blot Validation of SARS Nucleocapsid Antibody. Lane 1: SARS-CoV-2 Nucleocapsid protein, Lane 2: recombinant SARS Nucleocapsid protein, Lane 3: MERS Nucleocapsid protein probed with SARS Nucleocapsid Antibody [14B3D], NBP3-07063, at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37C.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-14B3D-Western-Blot-NBP3-07063-img0001.jpg "Western Blot: SARS Nucleocapsid Protein Antibody (14B3D) [NBP3-07063] - Western Blot Validation of SARS Nucleocapsid Antibody. Lane 1: SARS-CoV-2 Nucleocapsid protein, Lane 2: recombinant SARS Nucleocapsid protein, Lane 3: MERS Nucleocapsid protein probed with SARS Nucleocapsid Antibody [14B3D], NBP3-07063, at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37C.")
![Western Blot: SARS Nucleocapsid Protein Antibody (8G8A) [NBP3-07064] - Western Blot Validation of SARS Nucleocapsid Antibody. Lane 1: SARS-CoV-2 Nucleocapsid protein, Lane 2: recombinant SARS Nucleocapsid Protein and Lane 3: MERS Nucleocapsid protein probed with SARS Nucleocapsid Antibody [8G8A], NBP3-07064, at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37C.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-8G8A-Western-Blot-NBP3-07064-img0001.jpg "Western Blot: SARS Nucleocapsid Protein Antibody (8G8A) [NBP3-07064] - Western Blot Validation of SARS Nucleocapsid Antibody. Lane 1: SARS-CoV-2 Nucleocapsid protein, Lane 2: recombinant SARS Nucleocapsid Protein and Lane 3: MERS Nucleocapsid protein probed with SARS Nucleocapsid Antibody [8G8A], NBP3-07064, at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37C.")