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SARS Nucleocapsid Protein Antibody (05) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity VSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clone
05
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

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SARS Nucleocapsid Protein Antibody (05) [Janelia Fluor® 669] Summary

Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant SARS Nucleocapsid Protein (Accession#: NP_828858.1; Met1-Ala422).
Specificity
Has cross-reactivity in ELISA and WB with:
SARS-CoV-2 Nucleocapsid Protein

Has cross-reactivity in ELISA with:
SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid Protein
SARS-CoV-2 Nucleocapsid Protein
SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(R203K, G204R) Protein
SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(I292T) Protein
SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, R203K, G204R, S235F) Protein
SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, S235F) Protein
SARS-CoV-2 Alpha(B.1.1.7/ B.1.237) Nucleocapsid(S194L) Protein
SARS-CoV-2 Alpha/beta(B.1.1.7/ B.1.351/ A.2.2) Nucleocapsid(P13L) Protein
SARS-CoV-2 Beta(B.1.351/B.1.351.2/b.1.351.3/B.1.427/B.1.429) Nucleocapsid (T205I) Protein
SARS-CoV-2 Gamma(P.1/ P.1.1/ P.1.2) Nucleocapsid (P80R) Protein
SARS-CoV-2 Eta(B.1.525) Nucleocapsid (A12G, T205I) Protein
SARS-CoV-2 (B.1.617) Nucleocapsid (D377Y) Protein
SARS-CoV-2 (B.1.617) Nucleocapsid (R203M, D377Y) Protein
SARS-CoV-2 Delta(B.1.617.2) Nucleocapsid (D63G, R203M, D377Y) Protein
SARS-CoV-2 (B.1.617.3) Nucleocapsid (P67S, R203M, D377Y) Protein

No cross-reactivity in ELISA with:
MERS-CoV Nucleocapsid protein
HCoV-229E Nucleocapsid protein
HCoV-NL63 Nucleocapsid protein
HCoV-HKU1(isolate N5) Nucleocapsid protein
HCoV-OC43 Nucleocapsid protein
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
N
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for SARS Nucleocapsid Protein Antibody (05) [Janelia Fluor® 669]

  • N protein
  • N structural protein
  • N
  • NC
  • Nucleocapsid protein
  • Nucleoprotein
  • Protein N
  • SARS coronavirus N protein
  • SARS coronavirus nucleocapsid protein
  • SARS CoV N protein
  • SARS CoV nucleocapsid protein
  • SARS CoV
  • SARS N protein
  • SARS Nucleoprotein
  • SARSCoV N protein
  • SARSCoV nucleocapsid protein
  • SARSCoV
  • Severe acute respiratory syndrome

Background

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol N