![Western Blot: SARS Nucleocapsid Protein Antibody (05) [NBP3-06413] - Anti-SARS-NP mouse monoclonal antibody at 1:1000 dilution.Lane A: SARS-CoV NP Protein (30ng)Lane B: SARS-CoV NP Protein (5ng)Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (30ng)Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (5ng)Secondary: Goat Anti-Mouse IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-05-Western-Blot-NBP3-06413-img0001.jpg "Western Blot: SARS Nucleocapsid Protein Antibody (05) [NBP3-06413] - Anti-SARS-NP mouse monoclonal antibody at 1:1000 dilution.Lane A: SARS-CoV NP Protein (30ng)Lane B: SARS-CoV NP Protein (5ng)Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (30ng)Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (5ng)Secondary: Goat Anti-Mouse IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.")
| Reactivity | VSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC |
| Clone | 05 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant SARS Nucleocapsid Protein (Accession#: NP_828858.1; Met1-Ala422). |
| Specificity | Has cross-reactivity in ELISA and WB with: SARS-CoV-2 Nucleocapsid Protein Has cross-reactivity in ELISA with: SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid Protein SARS-CoV-2 Nucleocapsid Protein SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(R203K, G204R) Protein SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(I292T) Protein SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, R203K, G204R, S235F) Protein SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, S235F) Protein SARS-CoV-2 Alpha(B.1.1.7/ B.1.237) Nucleocapsid(S194L) Protein SARS-CoV-2 Alpha/beta(B.1.1.7/ B.1.351/ A.2.2) Nucleocapsid(P13L) Protein SARS-CoV-2 Beta(B.1.351/B.1.351.2/b.1.351.3/B.1.427/B.1.429) Nucleocapsid (T205I) Protein SARS-CoV-2 Gamma(P.1/ P.1.1/ P.1.2) Nucleocapsid (P80R) Protein SARS-CoV-2 Eta(B.1.525) Nucleocapsid (A12G, T205I) Protein SARS-CoV-2 (B.1.617) Nucleocapsid (D377Y) Protein SARS-CoV-2 (B.1.617) Nucleocapsid (R203M, D377Y) Protein SARS-CoV-2 Delta(B.1.617.2) Nucleocapsid (D63G, R203M, D377Y) Protein SARS-CoV-2 (B.1.617.3) Nucleocapsid (P67S, R203M, D377Y) Protein No cross-reactivity in ELISA with: MERS-CoV Nucleocapsid protein HCoV-229E Nucleocapsid protein HCoV-NL63 Nucleocapsid protein HCoV-HKU1(isolate N5) Nucleocapsid protein HCoV-OC43 Nucleocapsid protein |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | N |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | N |
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")