SARS Nucleocapsid Protein Antibody (05) [Alexa Fluor® 647] Summary
| Immunogen |
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant SARS Nucleocapsid Protein (Accession#: NP_828858.1; Met1-Ala422). |
| Specificity |
Has cross-reactivity in ELISA and WB with:
SARS-CoV-2 Nucleocapsid Protein
Has cross-reactivity in ELISA with:
SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid Protein
SARS-CoV-2 Nucleocapsid Protein
SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(R203K, G204R) Protein
SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(I292T) Protein
SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, R203K, G204R, S235F) Protein
SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, S235F) Protein
SARS-CoV-2 Alpha(B.1.1.7/ B.1.237) Nucleocapsid(S194L) Protein
SARS-CoV-2 Alpha/beta(B.1.1.7/ B.1.351/ A.2.2) Nucleocapsid(P13L) Protein
SARS-CoV-2 Beta(B.1.351/B.1.351.2/b.1.351.3/B.1.427/B.1.429) Nucleocapsid (T205I) Protein
SARS-CoV-2 Gamma(P.1/ P.1.1/ P.1.2) Nucleocapsid (P80R) Protein
SARS-CoV-2 Eta(B.1.525) Nucleocapsid (A12G, T205I) Protein
SARS-CoV-2 (B.1.617) Nucleocapsid (D377Y) Protein
SARS-CoV-2 (B.1.617) Nucleocapsid (R203M, D377Y) Protein
SARS-CoV-2 Delta(B.1.617.2) Nucleocapsid (D63G, R203M, D377Y) Protein
SARS-CoV-2 (B.1.617.3) Nucleocapsid (P67S, R203M, D377Y) Protein
No cross-reactivity in ELISA with:
MERS-CoV Nucleocapsid protein
HCoV-229E Nucleocapsid protein
HCoV-NL63 Nucleocapsid protein
HCoV-HKU1(isolate N5) Nucleocapsid protein
HCoV-OC43 Nucleocapsid protein |
| Isotype |
IgG1 |
| Clonality |
Monoclonal |
| Host |
Mouse |
| Gene |
N |
| Purity |
Protein A purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- ELISA
- Flow Cytometry
- Immunohistochemistry-Paraffin
- Western Blot
|
| Application Notes |
Optimal dilution of this antibody should be experimentally determined. |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
| Buffer |
50mM Sodium Borate |
| Preservative |
0.05% Sodium Azide |
| Purity |
Protein A purified |
Notes
Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for SARS Nucleocapsid Protein Antibody (05) [Alexa Fluor® 647]
Background
It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Secondary Antibodies
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Isotype Controls
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Additional SARS Nucleocapsid Protein Products
Blogs on SARS Nucleocapsid Protein
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")