![Western Blot: ZHX2 Antibody (JE63-11) [NBP3-33112] - Western blot analysis of ZHX2 on different lysates with Rabbit anti-ZHX2 antibody (NBP3-33112) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: COS-1 cell lysate Lane 3: HeLa cell lysate Lane 4: C6 cell lysate Lane 5: PC-12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: AGS cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 92 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33112) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33112_rabbit-zhx2-mab-je63-11-28820241419151.jpg "Western Blot: ZHX2 Antibody (JE63-11) [NBP3-33112] - Western blot analysis of ZHX2 on different lysates with Rabbit anti-ZHX2 antibody (NBP3-33112) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: COS-1 cell lysate Lane 3: HeLa cell lysate Lane 4: C6 cell lysate Lane 5: PC-12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: AGS cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 92 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33112) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | JE63-11 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within Human ZHX2 aa 251-500 / 837. (Uniprot: Q9Y6X8) |
| Localization | Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | ZHX2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 92 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for ZHX2 Antibody (NBP3-33112)Find related products by research area.
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| Gene Symbol | ZHX2 |
![Immunocytochemistry/ Immunofluorescence: ZHX2 Antibody (JE63-11) [NBP3-33112] - Immunocytochemistry analysis of MCF7 (positive) and AGS (negative) labeling ZHX2 with Rabbit anti-ZHX2 antibody (NBP3-33112) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ZHX2 antibody (NBP3-33112) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33112_rabbit-zhx2-mab-je63-11-29202410334077.jpg "Immunocytochemistry/ Immunofluorescence: ZHX2 Antibody (JE63-11) [NBP3-33112] - Immunocytochemistry analysis of MCF7 (positive) and AGS (negative) labeling ZHX2 with Rabbit anti-ZHX2 antibody (NBP3-33112) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ZHX2 antibody (NBP3-33112) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.")
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