![Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0014.jpg "Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ZEB2. The immunogen is located within the last 50 amino acids of ZEB2. Amino Acid Squence: DDSSEDGKMETKSDHEED |
| Specificity | ZEB2 antibody is predicted to not cross-react with ZEB1. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ZEB2 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 135 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
![Immunohistochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for ZEB2 Antibody (NBP1-77179)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: ZEB2 Antibody [NBP1-77179] - Jurkat cells at 20 ug/mL.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77179-img0011.jpg "Immunocytochemistry/Immunofluorescence: ZEB2 Antibody [NBP1-77179] - Jurkat cells at 20 ug/mL.")
![Western Blot: ZEB2 Antibody [NBP1-77179] - Analysis in EL4 cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0009.jpg "Western Blot: ZEB2 Antibody [NBP1-77179] - Analysis in EL4 cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Western Blot: ZEB2 Antibody [NBP1-77179] - Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, and then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0012.jpg "Western Blot: ZEB2 Antibody [NBP1-77179] - Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, and then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.")
![Western Blot: ZEB2 Antibody [NBP1-77179] - The protein levels of EMT-TFs were compared between carcinoma cell lines and non- or less-malignant tumor cell lines by using Western-blot analysis. The soft agar colony transformation or migration assay was referred to distinguish malignant degree of the experimental cancer cell lines (data not shown). Malignant degree: prostate cancer cell lines, P69 < M12; breast cancer cell lines, MCF7 < MD-MBA-231; lung cancer cell lines, A549 < H1299; liver cancer cell lines, HepG2 < SMMC7721. 'kD' (kilo-dalton) means the molecular weight of protein. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0013.jpg "Western Blot: ZEB2 Antibody [NBP1-77179] - The protein levels of EMT-TFs were compared between carcinoma cell lines and non- or less-malignant tumor cell lines by using Western-blot analysis. The soft agar colony transformation or migration assay was referred to distinguish malignant degree of the experimental cancer cell lines (data not shown). Malignant degree: prostate cancer cell lines, P69 < M12; breast cancer cell lines, MCF7 < MD-MBA-231; lung cancer cell lines, A549 < H1299; liver cancer cell lines, HepG2 < SMMC7721. 'kD' (kilo-dalton) means the molecular weight of protein. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: ZEB2 Antibody [NBP1-77179] - Jurkat cells with ZEB2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77179-img0002.jpg "Immunocytochemistry/Immunofluorescence: ZEB2 Antibody [NBP1-77179] - Jurkat cells with ZEB2 antibody at 20 ug/mL.")
![Immunocytochemistry/Immunofluorescence: ZEB2 Antibody [NBP1-77179] - Analysis of ZEB2 in Jurkat cells with ZEB2 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77179-img0010.jpg "Immunocytochemistry/Immunofluorescence: ZEB2 Antibody [NBP1-77179] - Analysis of ZEB2 in Jurkat cells with ZEB2 antibody at 20 ug/ml.")
![Bmal1 loss-of-function increases oxidative stress and Hif-1 alpha protein accumulation.(A) Immunoblots of Hif-1 alpha and Bmal1 protein levels in WT and M-BKO macrophages during a 24-hr time course of M1 activation. (B) Relative expression of Hif-1 alpha target genes in WT, M-BKO and M-HKO macrophages determined by qPCR. N = 3 biological replicates, statistical analysis was performed using two-way ANOVA for WT vs M-BKO or WT vs M-HKO across the time course. (C) Measurement of mROS using MitoSox Red (mean fluorescence intensity, MFI) in mitochondria isolated from control or M1-activated macrophages. Succinate (Suc, 10 mM) was included during MitoSox Red staining where indicated. N = 3 biological replicates, statistical analysis was performed using two-way ANOVA for WT vs M-BKO across the time course. (D) Hif-1 alpha protein levels in control or 8-hr M1-activated macrophages co-treated with or without N-acetylcysteine (NAC) or 10 mM dimethylmalonate (DMM). Data are presented as mean ± S.E.M. *, p<0.05 for WT vs M-BKO and p<0.05 for WT vs M-HKO. Experiments were repeated at least twice.Increased oxidative stress and Hif-1 alpha activity in M1 activated M-BKO macrophages.(A) Hif-1 alpha protein levels in M1-activated (primed with 10 ng/mL Ifn-gamma and stimulated with 50 ng/mL LPS) control and Bmal1-OE RAW264.7 stable lines. (B, C) Uptake of [3H]−2-deoxyglucose and lactate secretion in control or M1-activated macrophages. N = 3 biological replicates, statistical analysis was performed using Student’s T test. M-BHdko: myeloid-specific Bmal1 and Hif1a double knockout. (D) Glycolytic stress test in Ifn-gamma -primed macrophages measuring ECAR following glucose (25 mM) injection without or with LPS (100 ng/mL). Maximal glycolytic rate was determined by injection of oligomycin (OM, 2 μM), and glycolysis-dependent ECAR was determined by injection of 2-deoxyglucose (2-DG, 50 mM). The assay medium contained minimal DMEM with 2% dialyzed FBS, pH 7.4. N = 5 biological replicates. The difference between LPS-treated WT and M-BHdKO macrophages was determined by two-way ANOVA across the time course. (E) Expression of Nrf2 target genes determined by qPCR throughout a 24-hr time course following M1 stimulation. N = 3 biological replicates, statistical analysis was performed using two-way ANOVA for WT vs M-BKO across the time course. Data are presented as mean ± S.E.M. *, p<0.05. Experiments were repeated at least twice. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32396064), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-23220248215523.jpg "Bmal1 loss-of-function increases oxidative stress and Hif-1 alpha protein accumulation.(A) Immunoblots of Hif-1 alpha and Bmal1 protein levels in WT and M-BKO macrophages during a 24-hr time course of M1 activation. (B) Relative expression of Hif-1 alpha target genes in WT, M-BKO and M-HKO macrophages determined by qPCR. N = 3 biological replicates, statistical analysis was performed using two-way ANOVA for WT vs M-BKO or WT vs M-HKO across the time course. (C) Measurement of mROS using MitoSox Red (mean fluorescence intensity, MFI) in mitochondria isolated from control or M1-activated macrophages. Succinate (Suc, 10 mM) was included during MitoSox Red staining where indicated. N = 3 biological replicates, statistical analysis was performed using two-way ANOVA for WT vs M-BKO across the time course. (D) Hif-1 alpha protein levels in control or 8-hr M1-activated macrophages co-treated with or without N-acetylcysteine (NAC) or 10 mM dimethylmalonate (DMM). Data are presented as mean ± S.E.M. *, p<0.05 for WT vs M-BKO and p<0.05 for WT vs M-HKO. Experiments were repeated at least twice.Increased oxidative stress and Hif-1 alpha activity in M1 activated M-BKO macrophages.(A) Hif-1 alpha protein levels in M1-activated (primed with 10 ng/mL Ifn-gamma and stimulated with 50 ng/mL LPS) control and Bmal1-OE RAW264.7 stable lines. (B, C) Uptake of [3H]−2-deoxyglucose and lactate secretion in control or M1-activated macrophages. N = 3 biological replicates, statistical analysis was performed using Student’s T test. M-BHdko: myeloid-specific Bmal1 and Hif1a double knockout. (D) Glycolytic stress test in Ifn-gamma -primed macrophages measuring ECAR following glucose (25 mM) injection without or with LPS (100 ng/mL). Maximal glycolytic rate was determined by injection of oligomycin (OM, 2 μM), and glycolysis-dependent ECAR was determined by injection of 2-deoxyglucose (2-DG, 50 mM). The assay medium contained minimal DMEM with 2% dialyzed FBS, pH 7.4. N = 5 biological replicates. The difference between LPS-treated WT and M-BHdKO macrophages was determined by two-way ANOVA across the time course. (E) Expression of Nrf2 target genes determined by qPCR throughout a 24-hr time course following M1 stimulation. N = 3 biological replicates, statistical analysis was performed using two-way ANOVA for WT vs M-BKO across the time course. Data are presented as mean ± S.E.M. *, p<0.05. Experiments were repeated at least twice. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32396064), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")