![Western Blot: XRN2 Antibody [NB100-57541] - Detection of human XRN2 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN2 antibody NB100-57541 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/XRN2-Antibody-Western-Blot-NB100-57541-img0010.jpg "Western Blot: XRN2 Antibody [NB100-57541] - Detection of human XRN2 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN2 antibody NB100-57541 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 900 and 950 human 5'-3' exoribonuclease 2 using the numbering given in entry NP_036387.2 (GeneID 22803). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | XRN2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-57541 | Applications | Species |
|---|---|---|
| Barnhart MD, Moon SL, Emch AW et al. Changes in Cellular mRNA Stability, Splicing, and Polyadenylation through HuR Protein Sequestration by a Cytoplasmic RNA Virus. Cell Rep. 2013-11-06 [PMID: 24210824] (WB, Human) | WB | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||
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reviewed by:
Stephanie Moon |
WB | Human | 01/07/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: XRN2 Antibody [NB100-57541] - Sample: FFPE sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-XRN2 (NB100-57541). Detection: DAB](http://images.novusbio.com/fullsize/XRN2-Antibody-Immunohistochemistry-NB100-57541-img0006.jpg "Immunohistochemistry-Paraffin: XRN2 Antibody [NB100-57541] - Sample: FFPE sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-XRN2 (NB100-57541). Detection: DAB")
![Western Blot: XRN2 Antibody [NB100-57541] - Detection of mouse XRN2 by western blot. Samples: Whole cell lysate (15 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN2 antibody NB100-57541 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/XRN2-Antibody-Western-Blot-NB100-57541-img0009.jpg "Western Blot: XRN2 Antibody [NB100-57541] - Detection of mouse XRN2 by western blot. Samples: Whole cell lysate (15 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN2 antibody NB100-57541 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: XRN2 Antibody [NB100-57541] - Sample: FFPE sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-XRN2 (NB100-57541). Detection: DAB](http://images.novusbio.com/fullsize/XRN2-Antibody-Immunohistochemistry-NB100-57541-img0007.jpg "Immunohistochemistry-Paraffin: XRN2 Antibody [NB100-57541] - Sample: FFPE sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-XRN2 (NB100-57541). Detection: DAB")
![Immunoprecipitation: XRN2 Antibody [NB100-57541] - Detection of human XRN2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XRN2 antibody NB100-57541 used for IP at 3 ug per reaction. XRN2 was also immunoprecipitated by another rabbit anti-XRN2 antibody. For blotting immunoprecipitated XRN2, NB100-57541 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/XRN2-Antibody-Immunoprecipitation-NB100-57541-img0008.jpg "Immunoprecipitation: XRN2 Antibody [NB100-57541] - Detection of human XRN2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XRN2 antibody NB100-57541 used for IP at 3 ug per reaction. XRN2 was also immunoprecipitated by another rabbit anti-XRN2 antibody. For blotting immunoprecipitated XRN2, NB100-57541 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunocytochemistry/ Immunofluorescence SALL1 Antibody (K9814) [Unconjugated]](https://images.novusbio.com/images/pp-k9814-00_human-sall1-mab-clone-k9814-41202412514889.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")