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WDR77 Antibody (3F10)

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Western Blot: WDR77 Antibody (3F10) [H00079084-M01] - WDR77 monoclonal antibody (M01), clone 3F10 Analysis of WDR77 expression in HeLa.
Western Blot: WDR77 Antibody (3F10) [H00079084-M01] - WDR77 monoclonal antibody (M01), clone 3F10 Analysis of WDR77 expression in HeLa.
Immunohistochemistry-Paraffin: WDR77 Antibody (3F10) [H00079084-M01] - Analysis of monoclonal antibody to WDR77 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Western Blot: WDR77 Antibody (3F10) [H00079084-M01] - Analysis of WDR77 expression in transfected 293T cell line by WDR77 monoclonal antibody (M01), clone 3F10.Lane 1: WDR77 transfected lysate(36.7 KDa).Lane 2: ...read more
Sandwich ELISA: WDR77 Antibody (3F10) [H00079084-M01] - Detection limit for recombinant GST tagged WDR77 is approximately 3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
3F10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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WDR77 Antibody (3F10) Summary

Immunogen
WDR77 (AAH01679, 1 a.a. ~ 342 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRKETPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGALLLGASSLSGRCWAGSLWLFKDPCAAPNEGFCSAGVQTEAGVADLTWVGERGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVSVLSSGTQAVSGSKDICIKVWDLAQQVVLSSYRAHAAQVTCVAASPHKDSVFLSCSEDNRILLWDTRCPKPASQIGCSAPGYLPTSLAWHPQQSEVFVFGDENGTVSLVDTKSTSCVLSSAVHSQCVTGLVFSPHSVPFLASLSEDCSLAVLDSSLSELFRSQAHRDFVRDATWSPLNHSLLTTVGWDHQVVHHVVPTEPLPAPGPASVTE
Specificity
WDR77 - WD repeat domain 77
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
WDR77
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Publications
Read Publications using H00079084-M01.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for WDR77 Antibody (3F10)

  • Androgen receptor cofactor p44
  • MEP-50
  • MEP50Nbla10071
  • methylosome protein 50
  • MGC2722
  • p44
  • p44/Mep50
  • RP11-552M11.3
  • WD repeat domain 77
  • WD repeat-containing protein 77

Background

WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for WDR77 Antibody (H00079084-M01)(2)


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Bioinformatics

Gene Symbol WDR77
Entrez
Uniprot