Recombinant Human VPS28 Protein Summary
Description |
An un-tagged recombinant protein corresponding to the amino acids 1-221 of Human VPS28 Source: E.coli Amino Acid Sequence: MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
VPS28 |
Purity |
>95%, by SDS-PAGE |
Endotoxin Note |
< 1.0 EU per 1 microgram of protein (determined by LAL method) |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
25.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM Tris-HCl buffer (pH 7.5), 1 mM DTT, 10% glycerol |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
>95%, by SDS-PAGE |
Alternate Names for Recombinant Human VPS28 Protein
Background
VPS28 is involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. This protein is a subunit of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. VSP28 binding with the TSG101 may play important roles in budding of Retroviruses, such as HIV-1 and Ebola virus. Recombinant human VPS28 was expressed in E.coli and purified by using conventional chromatography.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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